The invention provides an automatic adjustment mechanism for needle-shuttle cooperation. The automatic adjustment mechanism comprises a main motor, an upper shaft, a cloth needling and thread take-upcomponent provided with a needle bar, a lower shaft, a rotating shuttle, a needle bar height adjustment drive source, a rotating shuttle angle adjustment drive source, a rotating shuttle position adjustment drive source, a needle bar height adjustment mechanism, a rotating shuttle angle adjustment mechanism and a rotating shuttle position adjustment mechanism, wherein the rotating shuttle angle adjustment mechanism comprises a rotary arm, an angle adjustment output shaft and a first transmission component connected between the rotary arm and the angle adjustment output shaft; the needle bar height adjustment drive source drives the needle bar to move in a vertical direction through the needle bar height adjustment mechanism; the rotating shuttle angle adjustment drive source sequentially drives the lower shaft to rotate around the own axis through the rotary arm, the first transmission component, the angle adjustment output shaft and a second transmission component; and the rotating shuttle position adjustment drive source drives the lower shaft to move in the own axial direction through the rotating shuttle position adjustment mechanism. According to the automatic adjustment mechanism, the height of the needle bar, the angle of the rotating shuttle and the left and right positions of the rotating shuttle can be automatically adjusted, and the automatic adjustment of the needle-shuttle cooperation is realized.