The invention discloses a preparation method of soluble polymers prepared by compound
enzyme modified
whey protein and belongs to the technical field of
protein preparations. The invention aims to solve the technical problems that
viscosity changes of existing
enzyme modification induced gel are not obvious or slow in
growth speed with the prolonging of the refrigerating time and gelling ability is poor, so that the gel is not suitable for application. The method comprises the following steps:
whey protein solution is stirred and pre-heated,
protease is added for hydrolyzing, after being hydrolyzed, the hydrolyzed
whey protein is sealed with a preservation film, the sealed hydrolyzed whey
protein is heated instantly to enable the enzymes to be inactivated, the heated hydrolyzed whey
protein is cooled with water to 50 DEG C, 5.0 U / g of mTGase is added, the mixture is reacted for 1 h, the mixture is heated to 85 DEG C for 5 min to enable the
enzyme to be inactivated, and the obtained sample is cooled to a
room temperature to obtain the soluble polymers. The whey protein soluble polymers produced by the method are transparent in appearance, suitable in
viscosity, easy to apply, stable and high in gel properties.