The invention relates to an antibacterial low-melting-point hot melt adhesive. The antibacterial low-melting-point hot melt adhesive is processed from the following raw materials in parts by weight: 40-80 parts of a polyester hot melt adhesive, 20-30 parts of polyamide resin, 3-8 parts of paraffin, 0.5-1 part of an antioxidant 164 and 0.5-1 part of an inorganic antibacterial agent. A preparation method of the antibacterial low-melting-point hot melt adhesive comprises the following steps: uniformly mixing the polyester hot melt adhesive, the polyamide resin, the paraffin, the antioxidant and the inorganic antibacterial agent according to the formula ratio, adding the mixture into a screw extruder granulator, carrying out melt blending extrusion, cooling and granulating the extruded mixture, and carrying out vacuum drying on the aggregates to remove moisture, so as to obtain the antibacterial low-melting-point hot melt adhesive. By mixing the low-melting-point polyester hot melt adhesive with the polyamide resin, the paraffin, the antioxidant and the inorganic antibacterial agent, the prepared hot melt adhesive is stable in binding power, high in curing speed and convenient to use and does not easily mildew, the environmental influence is low, and particularly, the curing rate is prior to that of the hot melt adhesive in the prior art in a low-temperature environment; the production efficiency is greatly improved, the cooling curing time is substantially shortened after the coating of the hot melt adhesive, and the coating efficiency is remarkably improved.