The invention provides a blood glucose testing instrument and the producing method thereof, which belongs to the field of bio-sensor technology. The substrate of the testing paper is printed with working electrode, reference electrode and electrical circuit which are formed by carbon-coating, one end of the substrate is printed with wire connected with readout instrument, the working electrode and reference electrode are respectively printed on carbon-coating above substrate conductive silver layer, the working electrode is also printed with enzyme, medium and adhesive. According to bio-sensor and electrochemical principle, the testing paper is made through silk-screen process, not only conductive circuit is printed, but also sensitive bio-element is directly printed on electrode supporter, the print amount of the print material and print area are totally and exactly controlled by computer. Once the testing paper contacts human body fluid, the testing result can be easily and directly read out on corresponding matching instrument, the producing method is easy, and has high precision, low cost, which is convenient for later process and encapsulation, and is good for large-scale production.