The invention relates to the fields of
traditional Chinese medicine and
biotechnology.
Isinglass bioactive peptide is prepared from fresh or dried
isinglass as raw materials, and by crushing, pulp homogenization, enzymolysis, centrifuging, sterilization and
ultrafiltration. A preparation method comprises adjusting the pH of
isinglass after the crushing and homogenization to 6-8; adding
flavor protease,
neutral protease and
papain (1:1:1) to the
isinglass and performing enzymolysis, wherein 5000-10000
enzyme activity units (u) of enzymes are added to each
gram of the isinglass, and a temperature is controlled to be 50-60 DEG C, and enzymolysis time is 3-6 hours; conducting
enzyme deactivation, the centrifuging, the sterilization, and the
ultrafiltration so as to obtain an isinglass
bioactive peptide liquid with a molecular weight of 5000 daltons or lower; and preparing the isinglass
bioactive peptide liquid into freeze-dried
powder products, oral liquid products or
capsule products. Theisinglass bioactive
peptide has the pharmacological activity of invigorating the
kidney, replenishing essence, nourishing yin, beautifying skins, nourishing tendons and veins, strengthening body functions, relieving fatigue quickly, and promoting
wound healing. The method is scientific and precise in working procedures, high in
operability, and beneficial for industrial production.