The invention discloses a bisexual flower breeding method of
manihot esculenta grant, and belongs to the technical field of agricultural cultivation. The breeding method comprises steps as follows: planting, top application, secondary top application,
hormone spraying, nutritional regulation before flowering, pinching,
pollen viability determination, crossbreeding,
pollination,
selfing isogeneity and final-period management. With the adoption of the breeding method, on the one hand, the problem of the small quantity of pollinating female flowers during crossbreeding of
manihot esculenta grant is solved, more female flowers with different genotypes are provided, and the crossbreeding efficiency is improved; on the other hand, the bisexual flower of
manihot esculenta grant is discovered for the first time, and an effective isogenic method adopting a
manihot esculenta grant inbred line is provided; and further, the bisexual flower is formed through induction for the first time in the
manihot esculenta grant breeding technology, the bisexual flower is used for crossbreeding, a novel crossbreeding method is created, and a new field is created for
manihot esculenta grant hybrids.