38results about How to "Avoid freeze damage" patented technology

The invention relates to the field of biological technology, and particularly relates to a mononuclear cell frozen stock solution of cord blood, an application and a preparation method. The mononuclear cell frozen stock solution of cord blood provided by the invention comprises dimethyl sulfoxide (DMSO) united plasmalyte force A (compound electrolyte injection) and hydroxyethyl starch (HES). Compared with the traditional formula, the scheme can be used for effectively preventing the pollution of animal derived pathogens and avoiding using of a cell culture medium to serve as a component of the frozen stock solution and guaranteeing a good cell frozen stock effect; after cell frozen for 2 months, the average cell viability of resurgent CBMC cell frozen by the cell frozen solution is 94.73%, which is remarkably (P is smaller than 0.05) higher than the cell frozen effect of the traditional cell frozen solution (10%DMSO+90%FBS). The resurgent cell can be directly applied to clinical transplantation, so the mononuclear cell frozen stock solution of the cord blood has a large practical value in clinical application of cell therapy.

The invention provides an anti-freezing system and method for a zero-power operation air cooling island of a low-pressure cylinder direct of an air cooling unit. The system comprises a steam turbine,an exhaust device and an air cooling island which are connected sequentially, one branch of a steam output pipeline of the steam turbine is connected with the gas inlet end of a low-pressure cylinderof the steam turbine through a second valve group; and the other branch of the output steam pipeline of the steam turbine is connected to the air cooling island through a third valve group and a temperature and pressure reducer which are connected in series, and the temperature and pressure reducer controls the steam quantity transmitted to the air cooling island according to the set steam quantity required by the air cooling island and the environment temperature. According to the invention, the temperature and pressure reducer is arranged to introduce steam in a heat supply output pipeline of the steam turbine into the air cooling island; the amount of steam input into the air cooling island is controlled according to the required steam amount, a closed vacuumizing and condensation watersystem prevents a small amount of steam from leaking into the shutdown air cooling island cooling unit, and freezing damage of the air cooling island during zero-power operation of the low-pressure cylinder can be avoided.

The invention relates to a serum-free freezing medium for an MDBK cell. The serum-free freezing medium comprises the components by volume percentag: 9-11% of dimethyl sulfoxide, 1-3% of a dextran 40 solution and the balance of DMEM high-glucose medium, wherein the dextran 40 solution is a DMEM high-glucose medium solution of 10% dextran 40; the 1-3% of dextran 40 solution can be replaced with 6-8% of a trehalose solution; and the trehalose solution is a DMEM high-glucose medium solution of 1mol / L trehalose. According to the freezing medium, serum with an animal source is not added, so that virus pollution of the animal source is avoided, and the cell viability after recovery is basically equal to that of the medium to which bovine serum is added.

The present invention aims to provide an intelligent system for zoning and precisely controlling temperature, humidity and light intensity in a photovoltaic greenhouse. The system comprises a wet curtain installed on one side wall in the photovoltaic greenhouse, water inlets are arranged above the wet curtain, and a water storage tank and a circulating water pump are arranged outside the greenhouse. A plurality of fans is arranged and installed on two side walls in the photovoltaic greenhouse. The system also includes several sunshades distributed and installed above the photovoltaic greenhouse. A large database is established for crop growth in the photovoltaic greenhouse. The database comprises optimal temperature, humidity, and light intensity data needed in the plant and crop growth, the data is transmitted to a data converter, a data processor and a central processor through sensors, the data is compared, thus, the data is transmitted to a controller to control the water flow of the wet curtain, the air volume of the fans and the lifting and lowering of the sunshades, and the intelligent control of environmental factors in the greenhouse is achieved. According to the system, the growth of crops in the greenhouse is facilitated, the three-dimensional production of agriculture is achieved, and the system is a high-tech modern agricultural ecological construction project withthe integration of low carbon, energy saving and environmental protection.

The invention discloses a cold and warm box system for an LNG fuel vehicle. The cold and warm box system comprises a cold and warm box shell, a vehicle main gas supply flow path, an LNG cold energy recycling flow path, a refrigerant circulation flow channel, an engine cooling liquid flow path and a cold energy flow path for other purposes. According to the cold and warm box system for the LNG fuel vehicle, under the situation that the engine cooling liquid flow path is not affected, and the additional energy consumption is not needed, the cooling capacity in the LNG gasification process can be recycled to conduct refrigeration, and the quantity of heat of an engine cooling liquid circulation system can be utilized to conduct heating; meanwhile, cold and heat switching and optimal temperature control under two kinds of modes are achieved at the same time, and thus the requirements for refrigeration in summer and heating in summer of a driver are met.

The invention discloses a strength inducer for a low-temperature cement-based material. The strength inducer comprises the following components in percentage by mass: 60-70% of a strength rapid development component; 7%-18% of a rheological component; 15%-25% of a micro-expansion component; 0.5%-1% of a coagulation adjusting component; and 1%-10% of an anti-freezing component. The inducer provided by the invention can shorten the setting time of a cement-based material under a low-temperature condition, rapidly develop the strength in a short time, have high early strength, and can realize early demolding and shorten the construction period. The conductive agent promotes the rapid development of the strength of the cement-based material, can avoid the freezing injury phenomenon, and can enhance the waterproof effect and the anti-corrosion effect at the same time. The components are wide in source and low in price, and the use cost is low. The additive is added when a cement-based material is mixed, can play a role after being uniformly stirred, and is convenient to use.

The invention discloses a peripheral blood mononuclear cell serum-free freezing medium. The peripheral blood mononuclear cell serum-free freezing medium is prepared from the following components in percentage by volume: 5 to 20% of dimethyl sulfoxide, 0.5 to 10% of dextran 40, and the balance of plasmalyte A. The invention further discloses a method for freezing peripheral blood mononuclear cells through the peripheral blood mononuclear cell serum-free freezing medium. Compared with the prior art, the freezing medium is free of animal serum, human serum and a cell culture medium, and has a good freezing effect; the cells can be directly applied to the clinic after resuscitation and can be also induced in vitro into immune cells NKT and CIK and have high application value in the clinic.

The invention provides an efficient energy tower solution heat pump unit system. The system comprises an energy tower, an evaporator, a solution transfer tank, a spraying freezing box and an ice water storage tank; a low-noise draught fan, a spraying device, a heat exchange layer and a liquid storage pool are arranged in the energy source tower, and the spraying freezing box comprises a freezing chamber and an ice water separation chamber; the system is simple in structure and reasonable in design, the regeneration technology that the solution is continuously purified from a dilute solution to become a high-concentration solution is achieved, the phenomenon that due to the fact that the heat exchange solution is dilute, and the ice point is reduced, the energy tower is damaged by freezing is avoided, efficient operating of the system is ensured, meanwhile, losses of the het exchange solution and the maintaining cost of equipment are saved, meanwhile, the system efficiently and stably runs, extra energy is not needed, the system is economical and practical, heating and refrigerating are completed through one step, and the system is simple, easy to implement and suitable for large-range popularization.

The invention relates to the field of refrigerating technology and fruit and vegetable fresh keeping, in particular to a special refrigerated cabinet for near-freezing-point refrigeration of fruits and vegetables and a temperature control method for the refrigerated cabinet. The special refrigerated cabinet for near-freezing-point refrigeration of the fruits and vegetables comprises a cabinet door (3), a cabinet body (16) and a refrigeration device. The cabinet body (16) is a closed rectangular container with an opening end. The refrigeration device comprises an insulating layer (1), a refrigerating plate (2), a first heat isolation layer (5) and a second heat isolation layer (6). A first channel (21) is formed between the second heat isolation layer (6) and the first heat isolation layer (5), and a second channel (22) is formed between the first heat isolation layer (5) and the refrigerating plate (2). The special refrigerated cabinet for near-freezing-point refrigeration of the fruits and vegetables is high in temperature control precision, uniform in storage chamber temperature and high in reliability and has an overtemperature early warning mechanism. Two sets of temperature control measures are designed, and the special refrigerated cabinet for near-freezing-point refrigeration of the fruits and vegetables and the temperature control method for the refrigerated cabinet have the advantages that the temperature control precision is high, the temperature in a storage room is uniform, overtemperature early warning can be achieved, and reliability is high.

The invention discloses a deep purification device and method of electrolytic aluminum exhaust gas. An electrolytic aluminum purification tower is provided with a gypsum slurry circulating region, aninlet flue, a spray layer of the purification tower, a first demister of the purification tower, a liquid accumulation layer of the purification tower, a condensation spray layer of the purification tower, a second demister of the purification tower and a purification tower outlet from bottom to top; the gypsum slurry circulating region is communicated with the spray layer of the purification tower through a purification tower circulating pump; the outlet end of the liquid accumulation layer of the purification tower is communicated with the gypsum slurry circulating region and a limestone slurry circulating tank; the limestone slurry circulating tank is communicated with the condensation spray layer of the purification tower through a circulating cooling tower; the limestone slurry circulating tank and the gypsum slurry circulating region are communicated with a limestone slurry source; and the gypsum slurry circulating region is communicated with a gypsum slurry treatment system. Byfull use of a limestone slurry preparation system in a gas desulfurization system of an original power plant and an auxiliary system of a byproduct treatment system, the deep purification device adopts two-stage wet washing process to perform low-cost deep purification on the electrolytic aluminum exhaust gas purified by a dry method.

The invention belongs to a technique for qualitatively detecting plant-derived components in foods and beverages, in particular to a kit and a method for detecting carotene components in the foods and the beverages by using a real-time fluorescent PCR (Polymerase Chain Reaction) technique. The kit comprises a PCR amplification reactive solution A, UNG (unguentum) enzyme B and Taq enzyme C, wherein the PCR amplification reactive solution A contains 10*PCR reaction buffers, magnesium sulfate, dNTP (deoxy-Nucleotide Triphosphate) containing dUTP (deoxy-Uridine Triphosphate), an upstream primer of 5'-CGACAAGCAAGCTTTACTCCAA-3', a downstream primer of 5'-CGTCTGACACCCATGAGTCTGT-3' and a TaqMan probe 5'-FAM-TCAAAACAGCCTTGAAAAACCCCACCA-TRAMA-3'. The method for rapidly detecting carotene components comprises the steps of extraction of DNA (Deoxyribonucleic Acid) in the foods and beverages, real-time PCR amplification of the carotene components and result judgement. The method has the advantages of rapidity, strong specificity, high sensitivity, convenient operation and good repeatability.

The utility model discloses a fuel gas hot water device and an anti-freezing valve. The fuel gas hot water device comprises a heat exchanger, a water inlet pipe, a water outlet pipe and an anti-freezing mechanism, wherein the water inlet pipe and the water outlet pipe are connected with the heat exchanger; the anti-freezing mechanism is arranged on the water inlet pipe; the anti-freezing mechanismhas a normal state allowing water in the water inlet pipe to flow through and a turn-off state blocking the water inlet pipe from conveying water to the heat exchanger; and the anti-freezing mechanism is driven by internal pressure to be switched from the normal state to the turn-off state when an environment temperature is lower than a first preset temperature. By using the fuel gas hot water device and the anti-freezing valve, the heat exchanger can be prevented from being frozen and damaged under a condition that a water heater is powered off, and meanwhile a situation that a user home issoaked is avoided.

The invention discloses a device for culturing porphyra tenera in a deep water area. The device comprises a plurality of pairs of brackets, cylindrical foamed buoys, and a cultivation curtain for culturing porphyra tenera; two cylindrical foamed buoys are bound to a pair of brackets through vinylon ropes; holes are drilled in two ends of each bracket; a tied rope passes through the through holes in two ends of each pair of brackets to fix the brackets; two ends of the cultivation curtain are tied to brackets which are adjacent to each other and in different pairs; the plurality of brackets, the foamed buoys bound on the bracket, and the cultivation curtain tied on the brackets form a row of porphyra tenera culturing float raft. According to the device, the full-floating raft type culturing technology is adopted, and thus the interference of float sludge and wild alga can be obviously reduced, and the disease incidence can be greatly lowered down; the device is suitable for cultivation in the deep water area; the net can be sunned actively to avoid freezing injury; bamboo brackets for fixing the foamed buoys are wrapped with a layer of fiberglass materials, and therefore, the strength of the traditional bamboo poles can be improved, the corrosion of seawater to the bamboo poles can be reduced, and as a result, the service life of the bamboo poles can be obviously prolonged, and as a result, the cost can be decreased.

The invention belongs to a qualitative detection technology of plant derived components in the foods and the beverages, in particular to a primer / a probe group for detecting a carrot component in the foods and the beverages. In the invention, a group of specific primer and probe are designed aiming at antifreeze protein of the carrot and according to the conserved sequence of a gene thereof. The carrot component in the foods and the beverages can be rapidly, sensitively and specifically detected by using the primer and the probe and adopting a real-time fluorescence PCR technology. The primer and the probe can be provided with other reagents together in the form of a kit and are used for amplification reaction of nucleic acid. The method has simple operation and good repeatability.

The invention relates to a hydrogen fuel battery low-temperature storage device capable of reducing the influence of the environment on a battery pack. The hydrogen fuel battery low-temperature storage device comprises a protection case, one side of the protection case is provided with a sealing box door, the interior of the protection case is fixedly connected with a fixed screen plate, one side of the fixed screen plate is provided with a battery body, the other side of the fixed screen plate is fixedly connected with a gas collecting hood, one side of the gas collecting hood communicates with an exhaust pipe, a temperature sensor is arranged in the gas collecting hood, and the front end of the protection case is fixedly connected with a digital temperature controller. Moisture in the case can be adsorbed through a silica gel desiccant, so that dryness in the case is guaranteed, it is avoided that the battery is frozen and damaged in the low-temperature environment due to the fact that the moisture in the case is too large, the economic loss condition is effectively avoided, the humidity of the desiccant can be monitored in real time through a humidity sensor, and when the humidity is too high, the desiccant is dried timely by a dryer and then is utilized again, so that use is convenient, and waste of resources can be avoided at the same time.

The invention discloses a household water pump with a freeze protection function. The household water pump comprises a reducer and a water tank, the reducer is provided with a mounting seat, the water tank is arranged at the top of the reducer, a water inlet and a water outlet are formed in the water tank, a branched connection pipe is arranged on the water tank, a manual water valve is arranged on the branched connection pipe which is connected with a water storage bottle through a pipeline, and the water storage bottle is arranged on the base. By the arrangement, a pump shell of the household water pump is prevented from being frozen to cause frost damage; the household water pump is novel in design, simple in structure, simple to manufacture, economical and environment friendly, energy saving and convenient, simple to regulate and control, good in practicability, high in market value and convenient to promote and apply.

The invention relates to a circulating cooling water device applied to a petroleum driller braking system and an application method. The system comprises a water storing tank, a flow guide plate, a water supplementing loop, a water draining loop and a water returning loop, wherein a heat insulation board is arranged on the inner wall of the water storing tank, and the flow guide plate is arranged in the water storing tank; in case of pouring water into the water storing tank, a water source pipeline can be connected, and a seventh valve can be opened; if a pump A is used for supplying water, a third valve and a fourth valve are opened; if a pump B is used for supplying water, a fifth valve and a sixth valve are opened, a water source valve is opened, and the rest valves are closed; after a water pump is subjected to air exhausting, the pump A or the pump B is started, and a twelfth valve is gradually opened; when the water pouring is finished, the twelfth valve is closed, then the pump A or the pump B is stopped, and the rest opened valves are closed. The circulating cooling water device is low in manufacturing cost; a water storing part is manufactured from common steel and heat insulation material; the circulating water storing tank is free of extra heating in winter, so that the energy consumption can be saved; the water pump and a cooler are reasonably assembled and used, so that the cooling efficiency of the circulating cooling water can be greatly improved.

The invention relates to a circulating cooling water device applied to a petroleum driller braking system and an application method. The system comprises a water storing tank, a flow guide plate, a water supplementing loop, a water draining loop and a water returning loop, wherein a heat insulation board is arranged on the inner wall of the water storing tank, and the flow guide plate is arranged in the water storing tank; in case of pouring water into the water storing tank, a water source pipeline can be connected, and a seventh valve can be opened; if a pump A is used for supplying water, a third valve and a fourth valve are opened; if a pump B is used for supplying water, a fifth valve and a sixth valve are opened, a water source valve is opened, and the rest valves are closed; after a water pump is subjected to air exhausting, the pump A or the pump B is started, and a twelfth valve is gradually opened; when the water pouring is finished, the twelfth valve is closed, then the pump A or the pump B is stopped, and the rest opened valves are closed. The circulating cooling water device is low in manufacturing cost; a water storing part is manufactured from common steel and heat insulation material; the circulating water storing tank is free of extra heating in winter, so that the energy consumption can be saved; the water pump and a cooler are reasonably assembled and used, so that the cooling efficiency of the circulating cooling water can be greatly improved.