38results about How to "Avoid freeze damage" patented technology

The invention discloses a peripheral blood mononuclear cell serum-free freezing medium. The peripheral blood mononuclear cell serum-free freezing medium is prepared from the following components in percentage by volume: 5 to 20% of dimethyl sulfoxide, 0.5 to 10% of dextran 40, and the balance of plasmalyte A. The invention further discloses a method for freezing peripheral blood mononuclear cells through the peripheral blood mononuclear cell serum-free freezing medium. Compared with the prior art, the freezing medium is free of animal serum, human serum and a cell culture medium, and has a good freezing effect; the cells can be directly applied to the clinic after resuscitation and can be also induced in vitro into immune cells NKT and CIK and have high application value in the clinic.

The invention relates to the field of refrigerating technology and fruit and vegetable fresh keeping, in particular to a special refrigerated cabinet for near-freezing-point refrigeration of fruits and vegetables and a temperature control method for the refrigerated cabinet. The special refrigerated cabinet for near-freezing-point refrigeration of the fruits and vegetables comprises a cabinet door (3), a cabinet body (16) and a refrigeration device. The cabinet body (16) is a closed rectangular container with an opening end. The refrigeration device comprises an insulating layer (1), a refrigerating plate (2), a first heat isolation layer (5) and a second heat isolation layer (6). A first channel (21) is formed between the second heat isolation layer (6) and the first heat isolation layer (5), and a second channel (22) is formed between the first heat isolation layer (5) and the refrigerating plate (2). The special refrigerated cabinet for near-freezing-point refrigeration of the fruits and vegetables is high in temperature control precision, uniform in storage chamber temperature and high in reliability and has an overtemperature early warning mechanism. Two sets of temperature control measures are designed, and the special refrigerated cabinet for near-freezing-point refrigeration of the fruits and vegetables and the temperature control method for the refrigerated cabinet have the advantages that the temperature control precision is high, the temperature in a storage room is uniform, overtemperature early warning can be achieved, and reliability is high.

The invention discloses a device for culturing porphyra tenera in a deep water area. The device comprises a plurality of pairs of brackets, cylindrical foamed buoys, and a cultivation curtain for culturing porphyra tenera; two cylindrical foamed buoys are bound to a pair of brackets through vinylon ropes; holes are drilled in two ends of each bracket; a tied rope passes through the through holes in two ends of each pair of brackets to fix the brackets; two ends of the cultivation curtain are tied to brackets which are adjacent to each other and in different pairs; the plurality of brackets, the foamed buoys bound on the bracket, and the cultivation curtain tied on the brackets form a row of porphyra tenera culturing float raft. According to the device, the full-floating raft type culturing technology is adopted, and thus the interference of float sludge and wild alga can be obviously reduced, and the disease incidence can be greatly lowered down; the device is suitable for cultivation in the deep water area; the net can be sunned actively to avoid freezing injury; bamboo brackets for fixing the foamed buoys are wrapped with a layer of fiberglass materials, and therefore, the strength of the traditional bamboo poles can be improved, the corrosion of seawater to the bamboo poles can be reduced, and as a result, the service life of the bamboo poles can be obviously prolonged, and as a result, the cost can be decreased.

The invention belongs to a qualitative detection technology of plant derived components in the foods and the beverages, in particular to a primer / a probe group for detecting a carrot component in the foods and the beverages. In the invention, a group of specific primer and probe are designed aiming at antifreeze protein of the carrot and according to the conserved sequence of a gene thereof. The carrot component in the foods and the beverages can be rapidly, sensitively and specifically detected by using the primer and the probe and adopting a real-time fluorescence PCR technology. The primer and the probe can be provided with other reagents together in the form of a kit and are used for amplification reaction of nucleic acid. The method has simple operation and good repeatability.

The invention relates to a circulating cooling water device applied to a petroleum driller braking system and an application method. The system comprises a water storing tank, a flow guide plate, a water supplementing loop, a water draining loop and a water returning loop, wherein a heat insulation board is arranged on the inner wall of the water storing tank, and the flow guide plate is arranged in the water storing tank; in case of pouring water into the water storing tank, a water source pipeline can be connected, and a seventh valve can be opened; if a pump A is used for supplying water, a third valve and a fourth valve are opened; if a pump B is used for supplying water, a fifth valve and a sixth valve are opened, a water source valve is opened, and the rest valves are closed; after a water pump is subjected to air exhausting, the pump A or the pump B is started, and a twelfth valve is gradually opened; when the water pouring is finished, the twelfth valve is closed, then the pump A or the pump B is stopped, and the rest opened valves are closed. The circulating cooling water device is low in manufacturing cost; a water storing part is manufactured from common steel and heat insulation material; the circulating water storing tank is free of extra heating in winter, so that the energy consumption can be saved; the water pump and a cooler are reasonably assembled and used, so that the cooling efficiency of the circulating cooling water can be greatly improved.