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Primer and prober for detecting carrot component in foods and beverages

A carrot and probe technology, applied in the field of rapid detection of plant-derived components, can solve problems such as unidentification, discrepancies between product real attributes and labels, and reduction of raw material consumption

Inactive Publication Date: 2014-07-30
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After various raw materials are processed into finished food and beverage products, it is no longer possible to identify their composition from their appearance
Producers are motivated by profit, change the composition of the product without authorization, or mix in cheap substitutes, or directly reduce the amount of raw materials, resulting in the true attributes of the product not matching the label

Method used

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  • Primer and prober for detecting carrot component in foods and beverages
  • Primer and prober for detecting carrot component in foods and beverages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Follow the procedure below for testing:

[0034] (1) Extraction of DNA from carrot noodle samples to be tested

[0035]A. Weigh 0.1g of carrot noodles, cut into pieces, and transfer to a 1.5mL centrifuge tube. Add 600 μL of preheated lysis buffer, mix gently, and keep warm in a water bath at 65°C for 10 minutes;

[0036] B. Add an equal volume of 600 μL of phenol / chloroform into the tube, mix well by inverting up and down, and extract for 2 minutes;

[0037] C. Centrifuge at 12000g for 5min, draw the supernatant into a new centrifuge tube, add the same volume of sedimentation solution as the supernatant, mix well, leave at room temperature for 10min, centrifuge at 12000g for 5min, remove the supernatant, and keep the precipitate;

[0038] D. Add 60 μL of RNase to the precipitate, place it at 37°C for 2 minutes, mix it well with a pipette tip, dissolve the precipitate at 37°C, add 300 μL of buffer solution after 5 minutes, and mix up and down 10 times;

[0039] E. Tak...

Embodiment 2

[0054] Follow the procedure below for testing:

[0055] (1) Extraction of the DNA of the tomato rice flour sample to be tested

[0056] A. Weigh 0.1g of tomato rice flour and transfer it to a 1.5mL centrifuge tube. Add 600 μL of preheated lysis buffer, mix gently, and keep warm in a water bath at 65°C for 10 minutes;

[0057] B. Add an equal volume of 600 μL of phenol / chloroform into the tube, mix well by inverting up and down, and extract for 2 minutes;

[0058] C. Centrifuge at 12000g for 5min, draw the supernatant into a new centrifuge tube, add the same volume of sedimentation solution as the supernatant, mix well, leave at room temperature for 10min, centrifuge at 12000g for 5min, remove the supernatant, and keep the precipitate;

[0059] D. Add 60 μL of RNase to the precipitate, place it at 37°C for 2 minutes, mix it well with a pipette tip, dissolve the precipitate at 37°C, add 300 μL of buffer solution after 5 minutes, and mix up and down 10 times;

[0060] E. Take ...

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PUM

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Abstract

The invention belongs to a qualitative detection technology of plant derived components in the foods and the beverages, in particular to a primer / a probe group for detecting a carrot component in the foods and the beverages. In the invention, a group of specific primer and probe are designed aiming at antifreeze protein of the carrot and according to the conserved sequence of a gene thereof. The carrot component in the foods and the beverages can be rapidly, sensitively and specifically detected by using the primer and the probe and adopting a real-time fluorescence PCR technology. The primer and the probe can be provided with other reagents together in the form of a kit and are used for amplification reaction of nucleic acid. The method has simple operation and good repeatability.

Description

technical field [0001] The invention relates to a method for rapid detection of plant-derived components using nucleic acid amplification technology, in particular to a primer and probe sequence for real-time fluorescent PCR detection of carrot components. Background technique [0002] The continuous expansion of global economic integration and trade internationalization is both an opportunity and a challenge for developing China. At present, my country has become the fifth largest exporter of agricultural products in the world, and the import and export trade of agricultural products continues to grow. In order to protect their own economic interests and compete for the international market, developed countries and regions such as the United States, the European Union, and Japan have tried their best to pass various inspection items and strict or even harsh technical standards, and set up trade barriers for imported agricultural products, which has caused my country's agric...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11G01N21/64
Inventor 孙敏徐彪高宏伟林超刘彩霞
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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