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Serum-free freezing medium for MDBK cell

A culture medium and cell technology, applied in the field of cell and bioengineering, can solve problems such as the easy introduction of bovine virus, and achieve the effects of avoiding freezing damage, reducing damage, and avoiding virus contamination

Inactive Publication Date: 2017-07-14
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a serum-free freezing medium for MDBK cells, which is used to solve the problem that fetal bovine serum or calf serum needs to be added when freezing MDBK cells, and bovine-derived viruses are easily introduced

Method used

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  • Serum-free freezing medium for MDBK cell

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Embodiment 1

[0021] A serum-free cryopreservation medium for MDBK cells, comprising components and volume percentage concentrations of: 11% dimethyl sulfoxide, 2% dextran 40 solution, and the balance being DMEM high-glucose medium. Wherein, the dextran 40 solution is a DMEM high-sugar medium solution of dextran 40 with a concentration of 10% by mass percentage, which means that the DMEM high-sugar medium is used as a solvent to dilute the dextran 40 into a dextran 40 with a concentration of 10% by mass. solution.

Embodiment 2

[0023] A serum-free cryopreservation medium for MDBK cells, comprising components and volume percentage concentrations of: 10% dimethyl sulfoxide, 1% dextran 40 solution, and the balance being DMEM high-glucose medium. Wherein, the dextran 40 solution is a DMEM high-sugar medium solution of dextran 40 with a concentration of 10% by mass percentage, which means that the DMEM high-sugar medium is used as a solvent to dilute the dextran 40 into a dextran 40 with a concentration of 10% by mass. solution.

Embodiment 3

[0025] A serum-free cryopreservation medium for MDBK cells, comprising components and volume percent concentrations of: 9% dimethyl sulfoxide, 3% dextran 40 solution, and the balance being DMEM high-glucose medium. Wherein, the dextran 40 solution is a DMEM high-sugar medium solution of dextran 40 with a concentration of 10% by mass percentage, which means that the DMEM high-sugar medium is used as a solvent to dilute the dextran 40 into a dextran 40 with a concentration of 10% by mass. solution.

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Abstract

The invention relates to a serum-free freezing medium for an MDBK cell. The serum-free freezing medium comprises the components by volume percentag: 9-11% of dimethyl sulfoxide, 1-3% of a dextran 40 solution and the balance of DMEM high-glucose medium, wherein the dextran 40 solution is a DMEM high-glucose medium solution of 10% dextran 40; the 1-3% of dextran 40 solution can be replaced with 6-8% of a trehalose solution; and the trehalose solution is a DMEM high-glucose medium solution of 1mol / L trehalose. According to the freezing medium, serum with an animal source is not added, so that virus pollution of the animal source is avoided, and the cell viability after recovery is basically equal to that of the medium to which bovine serum is added.

Description

technical field [0001] The invention belongs to the technical field of cells and bioengineering, and relates to a serum-free freezing culture medium for MDBK cells. Background technique [0002] Cell culture technology is widely used in the research of life science research. In the experiment, it is necessary to freeze the cell line to preserve the seed and facilitate the subsequent experiments at different times. Cryopreservation of continuous cell lines can reduce genetic variation and morphological changes caused by subculture and avoid senescence or malignant transformation of limited cell lines. Cells are generally cryopreserved by adding glycerol or dimethyl sulfoxide (DMSO) and serum to the basal medium. These additives can protect cells against freezing damage caused by intracellular ice crystals and osmotic effects. Cryoprotectants refer to substances that can protect cells from freezing damage, and can generally be divided into two types: permeable and non-permeab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 朱战波刘通陈楠楠刘宇周金玲岳山王华欣赵静虎刘珊珊何泊宁张世勋王天刘增禄
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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