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Immune cell cryopreservation method

A technology of immune cells and cryopreservation method, which is applied in the field of immune cell cryopreservation, can solve the problems of immune cells being susceptible to freezing injury, the survival rate of immune cell recovery is low, and the cell damage is large, so as to improve the survival rate of recovery and protect the freezing injury. , the effect of prolonging survival

Inactive Publication Date: 2019-03-29
深圳光彩生命工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for cryopreservation of immune cells. The technical problems to be solved are: immune cells are susceptible to freezing damage, low safety, large damage to cells, and low recovery survival rate of immune cells

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0017] A method for cryopreserving immune cells, comprising the following steps:

[0018] Prepare the immune cell cryopreservation solution, which consists of the following raw materials:

[0019] Dimethyl sulfoxide 3mL, dextran 2mL, cell culture medium 15mL, human serum albumin 2mL, raffinose 2mL, glucose 2mL, propylene glycol 1mL and human autologous plasma 80mL;

[0020] Immune cells are cryopreserved using immune cell freezing solution.

[0021] In the above embodiment, the preparation of the human autologous plasma includes the following steps: placing human peripheral blood in a sterile centrifuge tube for centrifugation, taking the upper layer of plasma for inactivation, transferring the extinguished plasma to a new centrifuge tube, Then centrifuge to absorb the supernatant, which is human autologous plasma.

[0022] In the above examples, the cell culture medium is DMEM / F12 medium or RPMI-1640 medium.

[0023] In the above-mentioned embodiments, the glucose is purif...

Embodiment 2

[0025] A method for cryopreserving immune cells, comprising the following steps:

[0026] Prepare the immune cell cryopreservation solution, which consists of the following raw materials:

[0027] Dimethyl sulfoxide 7mL, dextran 6mL, cell culture medium 25mL, human serum albumin 4mL, raffinose 4mL, glucose 4mL, propylene glycol 3mL and human autologous plasma 60mL;

[0028] Immune cells are cryopreserved using immune cell freezing solution.

[0029] In the above embodiment, the preparation of the human autologous plasma includes the following steps: placing human peripheral blood in a sterile centrifuge tube for centrifugation, taking the upper layer of plasma for inactivation, transferring the extinguished plasma to a new centrifuge tube, Then centrifuge to absorb the supernatant, which is human autologous plasma.

[0030] In the above examples, the cell culture medium is DMEM / F12 medium or RPMI-1640 medium.

[0031] In the above-mentioned embodiments, the glucose is purif...

Embodiment 3

[0033] A method for cryopreserving immune cells, comprising the following steps:

[0034] Prepare the immune cell cryopreservation solution, which consists of the following raw materials:

[0035] Dimethyl sulfoxide 5mL, dextran 4mL, cell culture medium 20mL, human serum albumin 3mL, raffinose 3mL, glucose 3mL, propylene glycol 2mL and human autologous plasma 70mL;

[0036] Immune cells are cryopreserved using immune cell freezing solution.

[0037] In the above embodiment, the preparation of the human autologous plasma includes the following steps: placing human peripheral blood in a sterile centrifuge tube for centrifugation, taking the upper layer of plasma for inactivation, transferring the extinguished plasma to a new centrifuge tube, Then centrifuge to absorb the supernatant, which is human autologous plasma.

[0038] In the above examples, the cell culture medium is DMEM / F12 medium or RPMI-1640 medium.

[0039] In the above-mentioned embodiments, the glucose is purif...

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PUM

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Abstract

The invention relates to an immune cell cryopreservation method. The immune cell cryopreservation method comprises the following steps: immune cell cryopreservation liquid is prepared, wherein the immune cell cryopreservation liquid is prepared from the following raw materials in percentage by weight: 3-7 mL of dimethyl sulfoxide, 2-6 mL of dextran, 15-25 mL of a cell culture medium, 2-4 mL of human serum albumin, 2-4 mL of raffinose, 2-4 mL of glucose, 1-3 mL of propylene glycol and 60-80 mL of human autologous plasma; and immune cells are cryopreserved through the immune cell cryopreservation liquid. Compared with the prior art, the immune cells are effectively protected against freezing damage, safety is high, damage to the cells is small, and the recovery survival rate of the immune cells can be increased and can reach 96% or above.

Description

technical field [0001] The invention relates to the technical field of cryopreservation of cells, in particular to a method for cryopreservation of immune cells. Background technique [0002] The existing cell cryopreservation solution mostly uses DMSO combined with animal serum, most of which are 10% DMSO and 90% fetal bovine serum. However, due to the high content of DMSO, it has considerable toxicity, which is not good for the patient's body, and it is immune Cells are vulnerable to freezing damage, low safety, great damage to cells, and low recovery and survival rate of immune cells. Contents of the invention [0003] The object of the present invention is to provide a cryopreservation method for immune cells, and the technical problems to be solved are: immune cells are easily damaged by freezing, have low safety, great damage to cells, and low recovery and survival rate of immune cells. [0004] The technical solution of the present invention to solve the above-ment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 陈阳李杰
Owner 深圳光彩生命工程技术有限公司
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