92 results about "Tuberculosis diagnostics" patented technology

The invention belongs to the fields of immunology and cytobiology, relates to a tuberculosis immunodiagnosis molecular marker and vaccine use thereof, and in particular relates to use of any one or more of proteins, selected from amino acid sequences shown as SEQ ID NO: 1-4, in preparing a tuberculosis diagnostic agent; preferably, the tuberculosis is phthisis. The protein shown by any sequence in SEQ ID NO: 1-4 can serve as a tuberculosis (such as phthisis) immunodiagnosis molecular marker, and has good coincidence rate and reaction intensity; besides, the tuberculosis immunodiagnosis molecular marker has the potential for being applied to preparation of a tuberculosis vaccine.

The invention discloses mycobacterium tuberculosis ESAT6 serial recombinant fusion protein and application thereof. The invention provides a mycobacterium tuberculosis ESAT6 codon optimization method, a serial recombinant fusion method and a method for preparing the fusion protein, and also provides a method for manufacturing and applying the recombinant fusion protein in a whole-blood IFN-gamma tuberculosis diagnosis kit and a TCELL-SPOT tuberculosis infection diagnosis kit. The fusion protein has the advantages of high specificity, high sensitivity and the like, and the requirements of the tuberculosis (TB) infection clinical diagnosis are well met.

The invention relates to a tuberculosis diagnostic composition and application of the tuberculosis diagnostic composition, in particular provides the tuberculosis diagnostic composition which consists of optional two or three of antigen ESAT-6 derived polypeptide, antigen CFP-10 derived polypeptide and antigen Rv3615c derived polypeptide. The invention also provides application of the tuberculosis diagnostic composition in a specific T cellular immune reaction caused by the detection of mycobacterium tuberculosis infection in vitro and application of the preparation of a tuberculosis diagnostic agent. Compared with the existing diagnostic technology, the diagnostic cost is reduced, the detection design and the operation process are simplified, and the detection sensitivity is obviously improved.

The present invention relates to a DNA aptamer binding specifically to tuberculosis specific antigen 7.7 kDa (TB7.7), a biosensor for diagnosis of tuberculosis, comprising the same, and a method for providing information for diagnosis of tuberculosis. The present inventors found that not only does a DNA aptamer according to the present invention have specific binding potential to TB7.7 protein, but also the binding affinity is excellent. When used, the DNA aptamer of the present invention can be thus expected to exhibit greater stability than a conventional ELISA method using an antibody. Hence, the aptamer is expected to find useful applications in the development of compositions for tuberculosis diagnosis, biosensors for tuberculosis diagnosis, and information providing methods for tuberculosis diagnosis.

The invention relates to a carbohydrate chip for quickly diagnosing tuberculosis, which comprises a solid-phase carrier. The carbohydrate chip is characterized by further comprising a mycobacterium tuberculosis lipopolysaccharides antigen probe which is fixed on the solid-phase carrier through oximation reaction. The invention also relates to a preparation method of the carbohydrate chip and a tuberculosis diagnostic kit adopting the carbohydrate chip. The preparation method comprises the following steps: separating, extracting and purifying lipopolysaccharides in a mycobacterium tuberculosis cell wall; fixing LAM (lipoarabinomannan) and LM (lipomannan) probes in the solid-phase carrier, such as a nitrocellulose membrane or porous plate, and the like, by utilizing a method of oximation reaction, thereby obtaining the carbohydrate chip; and judging if the mycobacterium tuberculosis is infected, by bonding the carbohydrate chip with a corresponding antibody in blood serum and performing enzyme linked color-developing reaction. A cell wall lipopolysaccharides antigen with species specificity adopted by the carbohydrate chip only can be combined with a specific tuberculosis antibody, so that the detecting specificity and sensitivity of the tuberculosis are greatly increased.

The present invention relates to MHC-peptide complexes and uses thereof in the diagnosis of, treatment of or vaccination against a disease in an individual. More specifically the invention discloses MHC complexes comprising Mycobacterium tuberculosis antigenic peptides and uses there of.

The invention relates to tuberculosis diagnostic methods and devices that measure particular biomarker profiles in subjects and identify a subject having tuberculosis based on such profiles.