30 results about "Staphylococcus simulans" patented technology

A portion of the lysostaphin gene of Staphylococcus simulans has been cloned and overexpressed in the cytoplasm of E. coli to yield lysostaphin, in the absence of preprolysostaphin and prolysostaphin, under the transcriptional control of an IPTG-inducible promoter and a ribosome binding site. IPTG induction of the transformed host cells produces intracellular, soluble, mature lysostaphin (27 kDa), in the complete absence of preprolysostaphin and prolysostaphin. The mature lysostaphin so formed dose not require post-translational modification. The mature lysostaphin so formed can be used treat and prevent staphylococcal infections.

The invention discloses a fermentation agent for a Chinese air-dried sausage. The fermentation agent is characterized by consisting of lactic acid bacteria and staphylococcus, wherein the lactic acid bacteria are the combination of Lactobacillus delbrueckii subsp. delbrueckii, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus sake, Lactobacillus paralimentarius, Lactobacillus pentosus, Pediococcus acidilactici, Pediococcus dextrinicus and Pediococcus pentosaceus; and the staphylococcus is the combination of Staphylococcus xylosus, Staphylococcus simulans and Staphylococcus sciuri. The fermentation of the air-drying process of sausages is promoted by adding the fermentation agent, so that the sausages can be produced throughout the year regardless of regions, and the quality and stability of the product are improved at the same time.

The invention provides a kit for detecting staphylococcus hominis. The kit comprises a guide RNA specifically targeting a staphylococcus hominis rpoB gene, amplification primer pairs, hydrated TwistAmp basic kit reaction drying balls, an LbCas12a protein, a single-stranded DNA probe (ssDNA), a Ribonuclease Inhibitor and a buffer solution. The kit is used for detecting the staphylococcus hominis, the detection specificity is high, and the staphylococcus hominis can be distinguished from other staphylococcocci including staphylococcus aureus, staphylococcus epidermidis, staphylococcus warneri, staphylococcus capitis and staphylococcus haemolyticus. The RNA sequence is used for detection, the consumed time is about 1 hour, no PCR instrument is needed, the requirements for equipment are low and significantly lower than those of a traditional bacterial culture method or PCR sequencing method, and the clinical practical value is high.

This invention discloses a lysin that can kill many species of Streptococci. A new lysin, ClyR, was constructed by the gene splicing method. The ClyR can effectively kill different species of Streptococci, including Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus suis, Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus mutans, Streptococcus equi, and various Enterococci and Staphylococcus aureus. ClyR shows good stability and is not sensitive to EDTA and high concentration of NaCl. Moreover, the ClyR is active in a wide range of pH and maintains high activity in pH 5-11. Recombinant protein ClyR is well expressed in E. coli stain BL21 (DE3). High doses of ClyR showed no apparent toxicity in mice. Furthermore, administration of 0.8 mg per mouse once is able to completely protect the mouse infected with lethal doses of Group B Streptococci. The ClyR can be used alone or in combination with different forms of reagents and solutions, for the control of a variety of Streptococci and for the treatment of infections caused by these bacteria. It has a broad application prospect.

The Staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and a SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis-causing pathogens, S. aureus and coagulase negative staphylococci (S. chronogenes, S. epidermis, S. hyicus, S. simulans, S. warneri, and S. xylocus), making it a strong antimicrobial protein and an effective candidate for treating multidrug-resistant staphylococci. Lytic activity is maintained at the pH (6.7) and the ‘free’ calcium concentration (3 mM) of milk. Truncated endolysin-derived proteins, containing just the endopeptidase domain, also lyse staphylococci, in the absence of the SH3b-binding domain.

The invention relates to a staphylococcus simulans BP10. The staphylococcus simulans BP10 is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 16592. The invention also relates to a staphylococcus simulans BP10 suspension and a preparation method thereof. The staphylococcus simulansBP10 is prepared by inoculating the staphylococcus simulans BP10 into a LB (lysogeny broth) culture medium for cultivation and then performing centrifugal collection, washing and sterile water resuspension precipitation. The invention also relates application of the staphylococcus simulans BP10 to sausage fermentation and discloses a sausage fermenting agent. The sausage fermenting agent is composed of a lactobacillus plantarum b-2 suspension and the staphylococcus simulans BP10 suspension as a volume ratio of 3:1. When applied to sausage fermentation, the sausage fermenting agent can improvethe quality of fermented sausage products, increase the flavor of the products, improves the structural texture of the products, shorten the fermentation cycle and improve safety of the products.

The invention discloses staphylococcus warneri classified and named as staphylococcus warneri KR809427. The staphylococcus warneri KR809427 is preserved in the China Center for Type Culture Collection on October 31, 2016, and a preservation number is CCTCC NO:M 2016599. The invention further discloses application of staphylococcus warneri to increase of soil pH value and passivation of soil copper activity and discloses an application bacterial agent. The staphylococcus warneri CCTCC NO:M 2016599 grows fast and is high in resistance to copper, acids, alkalis and salts and capable of remarkably increasing culture medium pH value and reducing effective copper content of soil, thereby being high in potential for remediation of different types of copper contaminated soil.

The invention discloses a preparation technology of cantonese style sausage, belonging to field of food processing. The preparation technology of the cantonese style sausage comprises the steps of a. preparation of zymophyte, wherein the zymophyte comprises lactobacillus pentosus, pediococcus acidilactici, staphylococcus xylosus, staphylococcus simulans, micrococcus uarians and staphylococcus epidermidis; respectively culturing strains of the zymophyte for later use; b. preparation of raw materials; c. preparation of ingredients; d. pickling, namely, evenly mixing the raw materials and the ingredients, and then inoculating the strains of the zymophyte in sequence; and e. filling the sausage, and drying to obtain the finished product. After the preparation technology of the cantonese style sausage is adopted, the production time of the cantonese style sausage is greatly shortened, and the yield of the cantonese style sausage is increased; and the specially-made ingredients are adopted, so that the cantonese style sausage prepared by the method is better in flavor compared with the cantonese style sausage prepared by the traditional method.

The invention discloses a fluorescent chromogenic culture medium for detecting staphylococcus aureus. The fluorescent chromogenic culture medium is prepared from a nitrogen source, a carbon source, sodium chloride and an infectious microbe growth inhibitor. The fluorescent chromogenic culture medium is characterized by further being prepared from staphylococcus aureus chromogenic substrate 4-methyl umbelliferone-p-nitro beta-D-glucoside and beta-D-glucoside, non staphylococcus aureus chromogenic substrate paranitrophenol-alpha-D-galactopyranose and non staphylococcus aureus chromogenic substrate p-nitrophenol beta-D-glucuronide. The fluorescent chromogenic culture medium disclosed by the invention can provide a more convenient chromogenic scheme for distinguishing the staphylococcus aureusfrom other staphylococcus under the situation without color developing aid existence, can effectively distinguish the staphylococcus aureus from a part of non staphylococcus aureus and other infectious microbes and has a better selectivity to the staphylococcus aureus.

Isolation of an iron regulated, nme-gene operon (designated sbn) from Staphylococcus aureus (RN6390), responsible for the biosynthesis of staphylobactm, a novel S. aureus siderophore Methods for treating or preventing a disease or condition caused by S. aureus infection, as well as methods for identifying agents that inhibit the biosynthesis of staphylobactm or inhibit the expression of genes in said sbn operon are further disclosed.