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Staphylococcus aureus fluorescent chromogenic culture medium

A technology of chromogenic culture medium and staphylococcus, which is applied in the field of culture medium for microbial detection, can solve problems such as false positives, and achieve the effect of simplifying the process of bacterial chromogenic culture

Inactive Publication Date: 2018-02-23
黄增才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have found that some non-Staphylococcus aureus such as Staphylococcus saprophyticus, Staphylococcus lentus and Staphylococcus seri also exhibit the above characteristics, resulting in false positives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A fluorescent chromogenic medium for detecting Staphylococcus aureus, each 1000mL medium contains 3g of beef extract, 10g of peptone, 15g of agar, 10g of sodium chloride, 1.5g of bovine bile salt, 0.04mg of cefixime, 4- Methylumbelliferone-β-D-glucoside 2g / L, p-nitro β-D-glucoside 3 g / L, nitrophenol-α-D-galactopyranose content 3.5g / L, Nitrophenol β-D-glucuronide is 1g / L, and sodium pyruvate is 10g.

Embodiment 2

[0042] A fluorescent chromogenic medium for detecting Staphylococcus aureus, each 1000mL medium contains 3g of beef extract, 10g of peptone, 15g of agar, 10g of sodium chloride, 1.5g of bovine bile salt, 0.04mg of cefixime, 4- Methylumbelliferone-β-D-glucoside 3g / L, p-nitro β-D-glucoside 2 g / L, nitrophenol-α-D-galactopyranose content 2g / L, nitro Base phenol β-D-glucuronide is 1.5g / L, and sodium pyruvate is 8g.

Embodiment 3

[0044] A fluorescent chromogenic medium for detecting Staphylococcus aureus, each 1000mL medium contains 3g of beef extract, 10g of peptone, 15g of agar, 15g of sodium chloride, 1.5g of bovine bile salt, and 0.04mg of cefixime, 4 -Methylumbelliferone-β-D-glucoside 5g / L, p-nitroβ-D-glucoside 6 g / L, the content of nitrophenol-α-D-galactopyranose is 5g / L, Nitrophenol β-D-glucuronide is 0.6g / L, and sodium pyruvate is 5g.

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PUM

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Abstract

The invention discloses a fluorescent chromogenic culture medium for detecting staphylococcus aureus. The fluorescent chromogenic culture medium is prepared from a nitrogen source, a carbon source, sodium chloride and an infectious microbe growth inhibitor. The fluorescent chromogenic culture medium is characterized by further being prepared from staphylococcus aureus chromogenic substrate 4-methyl umbelliferone-p-nitro beta-D-glucoside and beta-D-glucoside, non staphylococcus aureus chromogenic substrate paranitrophenol-alpha-D-galactopyranose and non staphylococcus aureus chromogenic substrate p-nitrophenol beta-D-glucuronide. The fluorescent chromogenic culture medium disclosed by the invention can provide a more convenient chromogenic scheme for distinguishing the staphylococcus aureusfrom other staphylococcus under the situation without color developing aid existence, can effectively distinguish the staphylococcus aureus from a part of non staphylococcus aureus and other infectious microbes and has a better selectivity to the staphylococcus aureus.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a fluorescence color development culture medium for detecting Staphylococcus aureus. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) is an opportunistic pathogenic bacterium, the most common pathogenic bacterium in human suppurative infection, and also an important pathogenic bacterium of human beings. It is a representative of Gram-positive bacteria, which can cause many local suppurative infections, pneumonia, pseudomembranous enteritis, pericarditis, etc., and even systemic infections such as sepsis and sepsis. When Staphylococcus aureus contaminates starchy and water-rich foods, such as milk boxes, milk products, meat, eggs, etc., a considerable amount of enterotoxin can be produced after 8-10 hours under appropriate temperature conditions, which can cause various diseases. Symptoms of acute gastroenteritis. [0003] Staphylococ...

Claims

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Application Information

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IPC IPC(8): C12Q1/14C12R1/445
CPCC12Q1/14C12Q1/045C12Q2334/10C12Q2334/22
Inventor 黄增才
Owner 黄增才
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