96 results about "Riboflavinum" patented technology

The invention discloses a bacillus subtilis-coded PRPP (Phosphoribosyl Pyrophosphate) transamidase mutant gene pruF and an application thereof. The bacillus subtilis-coded PRPP transamidase mutant gene pruF sequence is shown as SEQ ID No.1. An engineering bacterium which is established and contains the bacillus subtilis-coded PRPP transamidase mutant gene pruF is biologically safe and clear in genetic background, so that the capacity of the bacillus subtilis which synthesizes riboflavin can be greatly improved, and the accumulation level of riboflavin can be improved by over 20%.

The present invention relates to a novel class of water compatible molecularly imprinted polymers (AquaMIPs) capable of selectively binding target molecules such as riboflavin, or analogues thereof, in water or aqueous media, their synthesis and use thereof in food processing and extraction or separation processes.

The invention discloses a method for inactivating blood component pathogens by a riboflavin photochemical method. The method for inactivating blood component pathogens by the riboflavin photochemicalmethod comprises the steps as follows: S1, adding a riboflavin solution with concentration of 50 [mu]mol / L to a blood product to obtain new blood; S2, transferring the blood product under illumination, removing dissolved oxygen in blood components by a nitrogen circulating system, and reducing production of oxygen free radicals; and S3, exposing the obtained new blood under narrow-spectrum ultraviolet light with the wave peak of 309 nm-313 nm, exciting riboflavin to have electron transfer, and damaging DNA and RNA in blood components to achieve the purposes of inactivating pathogens and lymphocytes. Equipment for inactivating blood component pathogens by the riboflavin photochemical method comprises illumination pretreatment equipment, illumination equipment and product storage equipment.The problems of pathogen spreading and leukocyte contamination risks in current blood transfusion are solved.

The invention belongs to the field of microbial fermentation, and discloses riboflavin-producing bacillus subtilis and a construction method and application thereof. According to the riboflavin-producing bacillus subtilis, at least one gene locus in purR, ribC or pyrE in a bacillus subtilis strain is mutated. The accumulated riboflavin of the bacillus subtilis strain obtained by mutating at leastone gene locus of purR, ribC or pyrE under the fermentation condition is significantly increased. The riboflavin-producing bacillus subtilis is a riboflavin high-yield strain, the produced riboflavinis significantly increased, and a foundation is laid for industrial production of the riboflavin.

The invention provides a yellow cucumber and a planting method thereof and a special light-scattering, cooling and heat radiation control film. The yellow cucumber is yellow, the riboflavin content is 0.079 mg / 100 g, and the pantothenic acid content is 0.38 mg / 100 g. The planting method comprises a step of covering a light shielding bag, wherein in the step of covering the light shielding bag, the light shielding bag made of the light-scattering, cooling and heat radiation control film is adopted, and the bottom end of the bag is provided with an air vent; a breather pipe is arranged at the air vent of the light shielding bag, and the breather pipe is made of a light-scattering material. The yellow cucumber disclosed by the invention is high in nutritive value, good in taste, and high in ornamental value.

The invention relates to the field of drug lead and discloses an application of riboflavin and riboflavin derivatives to specific triggering of DNA single-strand breaking for the first time. Riboflavin and the riboflavin derivatives can specifically recognize G-T mismatch in double-stranded DNA and trigger specific breaking of single-strand loci of DNA under illumination. The invention further discloses riboflavin and the riboflavin derivatives as cancer therapy targeted drugs and an application of the cancer therapy targeted drugs. Riboflavin and the riboflavin derivatives can result in breaking of genomic DNA in cancer cells without a high-level mismatch repair system for G-T mismatch, and finally, death of the cancer cells is caused. Riboflavin and the riboflavin derivatives are economical, safe, low-toxic and free of side effects when taken as cancer therapy drugs and applied to cancer targeted therapy.

The invention discloses photo-curable silk fibroin hydrogel and a preparation method thereof. The photo-curable silk fibroin hydrogel is prepared by: adding riboflavin and horse radish peroxidase intosilk fibroin solution that acts as a raw material, introducing oxygen continuously under ultraviolet irradiation and room temperature, inducing groups in silk fibroin macromolecules to chemically crosslink, and allowing gelling. The silk fibroin in the photo-curable hydrogel provided herein is mainly of random coil; the photo-curable hydrogel has high light transmittance, high compressive strength and high elasticity and is applicable to tissue repair materials.

The present invention provides a composition for three-dimensional printing, containing: a decellularized extracellular matrix; and riboflavin as a cross-linking agent. A three-dimensional structure having high mechanical strength can be manufactured by performing a layer-by-layer process through printing using the composition for three-dimensional printing according to the present invention and cross-linking under UVA to prepare a three-dimensional structure shape and then subjecting the thus obtained three-dimensional structure shape to thermal gelation. Furthermore, the present invention provides a method for preparing the composition for three-dimensional printing, and a method for manufacturing a three-dimensional structure using the composition for three-dimensional printing.

The invention discloses a two-component regulatory system mutant gene yvrH (G665A) of bacillus subtilis and application thereof. The two-component regulatory system mutant gene yvrH (G665A) of the bacillus subtilis is shown in SEQ ID No.1. The engineering bacteria containing the two-component regulatory system mutant gene yvrH (G665A) of bacillus subtilis built by the invention have biosafety and clear genetic backgrounds, the ability of the bacillus subtilis to synthesize riboflavin can be greatly improved by the contained mutant gene yvrH (G665A), and the riboflavin accumulation level can be improved by over 15% under a shake flask fermentation condition.

Ocular iontophoresis device and method for delivering any ionized drug solution to the cornea includes: a reservoir containing a solution suitable to be positioned on the eye; an active electrode disposed in or on the reservoir; and a passive electrode suitable to be placed on the skin of the subject, elsewhere on the body; elements for irradiating the cornea surface with suitable light for obtaining corneal cross-linking after the drug delivery; wherein the reservoir and the active electrode are transparent to UV light and / or visible light and / or IR light. The method includes: positioning the iontophoretic device on the eye to be treated; driving the solution by a cathodic current applied for 0.5 to 5 min, at an intensity not higher than 2 mA; and thereafter irradiating, with UV light for 5 to 30 min at a power of 3 to 30 mW / cm2; thereby obtaining the corneal cross-linking of the solution.

The invention discloses a photochemical inactivating system for circulating tumor cells and / or tumor stem cells in blood or blood products and application of riboflavin in preparation of an agent for inactivating the circulating tumor cells and / or the tumor stem cells in the blood or the blood products in a photochemical way. According to the system and application, the circulating tumor cells and / or the tumor stem cells in the peripheral blood are completely induced to be subjected to apoptosis to lose the proliferation ability, so that blood cells, coagulation function and immune function of a patient are maintained to the utmost extent.

The invention discloses a method for rapidly removing hexavalent chromium pollutants by using an electron carrier, and belongs to the technical field of environment. The physiological property that the Shewanella oneidensis MR-1 can be used for removing hexavalent chromium pollutants is used as a basis; a strain is cultured to a stable growth period; thalli are collected and transferred to a reactor to carry out re-suspending; the hexavalent chromium pollutant removal capability of the electron carriers namely riboflavin and anthraquinone-2-sodium sulfonate is measured; under the condition that the concentration of riboflavin is 10-50 [mu]M, the Cr<6+> removal rate of riboflavin is increased to 1.40-2.26 times, the removal rate is increased to 42.0-67.7% from 30.0%, and the Cr<6+> removalrate is higher under the condition that the pH value is equal to 8; anthraquinone-2-sodium sulfonate can also improve the removal speed and removal rate of Cr<6+>, and under a concentration of 100 [mu]M, the maximum removal rate reaches 98.1% and is close to 100%. Hexavalent chromium pollutants can be quickly removed by utilizing the electron carrier, so that the problem of low removal efficiencyof hexavalent chromium pollutants at present is solved, and the method has potential environmental remediation and application values.

The invention provides an inactivation device and inactivation method for a riboflavin photochemical blood product. The inactivation device can be arranged on an object placing surface (60). The inactivation device comprises a shell (10), a first light-emitting plate (13), a second light-emitting plate (16) and a movable supporting plate (19). The shell (10) is provided with an inactivation compartment (11) and a hatch (12). The inactivation chamber (11) has an extension direction that can be perpendicular to the object placing surface (60). And the hatch (12) is communicated with the inactivation compartment (11) and has a hatch orientation perpendicular to the extension direction. The invention also provides an inactivation method of the riboflavin photochemical blood product. With a mode of combination of riboflavin with light source irradiation, parasite, bacteria, viruses and white blood cells in the blood product are inactivated, so that the inactivation efficiency is improved and thus the safety of the blood product is improved. The structure is simple and the inactivation device is suitable for being used in various occasions.

The invention discloses recombinant shewanella algae capable of producing riboflavin and an application of the recombinant shewanella algae in electricity production, wherein the recombinant shewanella algae is constructed by the following method: (1) connecting five target genes ribA, ribC, ribD, ribE and ribH in a flavin synthetic route to a vector pyydt to obtain a recombinant plasmid R5; and (2) transferring the recombinant plasmid R5 into a shewanella algae scs-1 CGMCC No.18696 strain, so as to obtain the recombinant shewanella algae scs-R5 for producing riboflavin. According to the recombinant bacterium disclosed by the invention, the yield of riboflavin serving as an electron transfer body is high, so that the electricity generation capacity is improved.

The invention discloses a method and a fermentation device for increasing yield of riboflavin by using an oxygen carrier. The method comprises the steps as follows: S1, seed culture: inoculating a seed culture medium with a riboflavin producing bacillus subtilis strain for culture to obtain a seed culture solution; and S2, fermentation culture: inoculating a fermentation culture medium with the obtained seed culture solution, then, adding the sterile oxygen carrier, and performing fermentation to obtain the riboflavin. The proper oxygen carrier is added to the culture medium, culture is performed in the fermentation device, the dissolved oxygen concentration in the culture medium is increased by chemical and physical methods, thallus metabolism of the riboflavin producing bacillus subtilisis promoted, the substrate conversion rate is increased, the synthetic amount of riboflavin is increased from 11424 [mu]g / mL to 15820 [mu]g / mL, besides, the fermentation cycle is shortened, and the production cost is reduced.

The invention provides an engineering bacterium of Geobacillusthermoglucosidasius and a construction method and application of the engineering bacterium. The construction method includes: through genetic engineering of Geobacillusthermoglucosidasius, introducing ribC (G199D) point mutation, sequentially knocking out purR, purA, ccpN and ldh genes, and then introducing an expression vector carryinga riboflavin synthesis gene cluster into the transformed engineering bacteria to obtain a series of high-temperature riboflavin-producing geobacillus engineering bacteria. The results show that yieldof riboflavin after fermentation of geobacillus after genetic engineering for 24 h is 520 mg / L; and through mixed fermentation with glucose and xylose for the strain, the yield can reach more than 1g / L. The engineering bacterium has advantages of high growth temperature and short generation time, can utilize an inexpensive carbon source, and can be used for large-scale production of riboflavin at low cost.

The application discloses a preparation method of a composite cross-linked bioprosthetic valve. The preparation method comprises the following steps: 1, treating a decellularized bioprosthetic valve with tannic acid; 2, treating the tannic acid treated bioprosthetic valve with neomycin; 3, treating the neomycin treated bioprosthetic valve with 5-riboflavin monophosphate under the condition of ultraviolet irradiation; and 4, rinsing to obtain the composite cross-linked bioprosthetic valve. The application further provides a composite cross-linked bioprosthetic valve. According to the preparation method of the composite cross-linked bioprosthetic valve and the prepared composite cross-linked bioprosthetic valve, which are provided by the application, photooxidation of riboflavin is adopted and synergic with tannic acid and neomycin to carry out cross-linking on decellularized bovine pericardium, so that the aim of cross-linking three main components of an extracellular matrix is fulfilled, and the bioprosthetic valve which is of a good extracellular matrix structure, has an excellent mechanical effect and heat shrinkage temperature, is thin in thickness, can have a wider application range and has good collagen enzymolysis resistance and elastic enzymolysis resistance can be obtained.

The invention belongs to the technical field of biology, and particularly relates to a bacillus subtilis strain B13 and application thereof. According to the bacillus subtilis strain B13, the preservation number of the strain is CCTCC M 2020854, and the strain is preserved in the China Center for Type Culture Collection (CCTCC) on December 4, 2020. According to the bacillus subtilis strain B13 disclosed by the invention, after the strain is subjected to heavy ion mutagenesis, the shake flask fermentation yield of riboflavin is remarkably improved, and the bacillus subtilis strain B13 can be used as a strain for industrial production of enterprises and meets the requirements of the market on riboflavin.

The present invention relates to a substance selected from the group consisting of riboflavin, its esters, salts and hydrates thereof, for use in an ophthalmic diagnostic method. In preferred aspects of the invention, the riboflavin is applied into the eye as part of a solution having a concentration from about 0.01 and 0.5% riboflavin or acceptable salt, ester, hydrate thereof.

The present invention relates to a method of producing riboflavin in a genetically modified organism of the genus Eremothecium, wherein said genetic modification is linked to the fatty acid uptake and / or beta-oxidation pathway of said organism, comprising growing said organisms in a culture medium and isolating riboflavin from the culture medium. The invention further relates to a method of providing a riboflavin accumulating organism belonging to the genus Eremothecium by genetically modifying said organism, to organisms obtained by such a method, as well as the use of such genetically modified organisms for increasing the accumulation of riboflavin.

The invention relates to a method for increasing the yield of riboflavin produced by fermentation of bacillus subtilis, which belongs to the technical field of gene engineering. The method comprises the steps of carrying out genetic modification on a riboflavin production strain; removing the activation mechanism of intracellular high-level GTP on pyr operon; and stabilizing the expression of pyroperon at a low level to reduce the metabolic flux of pyrimidine nucleotide from the first synthesis route and increase the proportion of PRPP entering the purine nucleotide synthesis route. The yieldand yield coefficient of riboflavin are improved, and the fermentation cost of riboflavin is reduced.

The present invention is the method of utilizing the dark reaction of riboflavin and its derivative and its application. Riboflavin and its derivative is dispersed in the surfactant micelle solution and mixed with reductant with lower redox potential to obtain free radical generating dark reaction composition. The composition has the mass composition of riboflavin and its derivative to surfactant micelle solution to reductant with lower redox potential of 1 to 10-3000 to 0.1-20. Under dark condition, the composition can generate great amount of free radicals to suppress the growth of fungi effectively with less resistance. The present invention may be used as medicine for preventing and treating microbe caused diseases and diseases of crops, and may be also used in preparing bactericide and preparation for degrading dark environment pollutant. The present invention lays foundation of the wide application of riboflavin and its derivative.

The invention provides a method for improving riboflavin production capacity of escherichia coli engineering bacteria by using DNA shuffling, and relates to the field of genetic engineering, and the method comprises the following steps: constructing riboflavin-producing escherichia coli engineering bacteria containing 26 genes; carrying out DNA rearrangement and directional screening on the T7RNA polymerase gene expression unit, and extracting to obtain a first batch of positive plasmids; carrying out DNA rearrangement and directional screening on a riboflavin biosynthesis and transport system, and extracting to obtain a second batch of positive plasmids; carrying out DNA rearrangement and directional screening on the Escherichia coli sigma factor gene, and extracting to obtain a third batch of positive plasmids; introducing the third batch of plasmids into different chassis cells to obtain recombinant strains; and selecting the positive strain with the highest riboflavin yield. The toxicity problem of T7RNAP to an escherichia coli host in the construction process of the riboflavin-producing escherichia coli engineering bacteria, the feedback inhibition problem of a product and the moderate and coordinated expression problem of a constructed riboflavin biosynthetic gene are effectively eliminated. The efficiency and the yield of biosynthesis of riboflavin by the escherichia coli engineering strain are further improved.

The invention belongs to the field of microbial fermentation, and discloses a method for producing riboflavin. The method for producing riboflavin comprises a construction method of a riboflavin producing strain and a method for producing riboflavin by fermenting the constructed strain. The bacillus subtilis is characterized in that at least one gene locus of purR, ribR, ribC or pyrE in a bacillussubtilis strain is mutated. The accumulation of riboflavin in the bacillus subtilis strain obtained by mutation of at least one gene locus of purR, ribR, ribC or pyrE under the fermentation conditionis remarkably increased. The bacillus subtilis is a riboflavin high-yield strain, the produced riboflavin is remarkably increased, and a foundation is laid for industrial production of the riboflavin.

The invention provides a preparation method of fluorescent copper nanoparticles. Anhydrous copper sulfate serves as a copper source, ascorbic acid is added, and a low-temperature rotary evaporation closed system liquid-phase chemical reduction method is utilized to prepare the fluorescent copper nanoparticles. The invention also provides the fluorescent copper nanoparticles prepared through the method and application of the fluorescent copper nanoparticles in detecting the content of VB2 and sulfur ions. A copper nano-solution with blue fluorescence is synthesized first, copper nanoparticles have strong fluorescence at about 440 nm, riboflavin has strong ultraviolet absorption at about 450 nm, and the copper nanoparticles and riboflavin are almost completely overlapped, so that it is supposed that the resonance energy transfer (FERT) process occurs between the copper nanoparticles and riboflavin, and therefore a fluorescence resonance energy transfer system between the copper nanoparticles and riboflavin is established and can be used for detecting riboflavin. In the copper nanoparticle and riboflavin system, S2- reacts with VB2, so that fluorescence quenching of the copper nanoparticle and riboflavin system happens, and therefore the sulfur ions can be detected.

The invention discloses a photo-crosslinked chitosan-riboflavin edible film and a preparation method thereof. The preparation method comprises the following steps of weighing a certain amount of chitosan powder, and adding the chitosan powder into an acetic acid solution to prepare a chitosan solution, adding a certain amount of glycerol into the chitosan solution as a plasticizer, then adding a proper amount of riboflavin powder under a dark condition, and uniformly stirring to obtain a mixed solution, removing bubbles from the mixed solution, casting the mixed solution on a flat plate, and irradiating the flat plate under ultraviolet light to form a film, and finally, drying the film on the flat plate, and stripping from the flat plate to obtain the film. Chitosan and riboflavin are used as raw materials, the edible film which is green, non-toxic, environmentally friendly and good in stability is prepared, the molecular property of chitosan is improved through a photo-crosslinked method, and under excitation of illumination, the chitosan edible film which is good in physical and chemical performance and remarkably improved in antibacterial effect is prepared.

A frangible container to be attached to the tip of an arrow, the container containing riboflavin so that when the container is attached to the tip of an arrow and the arrow is fired at and hits a game animal to wound the game animal, the frangible container breaks so that the riboflavin is distributed into the environment where the animal was hit and into the blood flowing from the wound so that the environment where the animal was hit and the blood trail from the blood flowing from the wound can be more readily detected by directing light having a wavelength in the range of from about 300 to about 500 nanometers to observe the resulting florescence of the riboflavin in the environment where the animal was hit and in the blood trail. In addition, a combination of an arrowhead and a frangible container wherein the frangible container is attached to or contained within the arrowhead.