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Inactivating system for circulating tumor cells in blood and application thereof

A technology of tumor cells and tumor stem cells, which is applied in the system field of riboflavin photochemical method to inactivate circulating tumor cells and/or tumor stem cells, and can solve the problems of dissemination and shedding of tumor cells into the blood, etc.

Pending Publication Date: 2017-11-03
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] One object of the present invention is to solve the problem of unavoidable hematogenous dissemination of tumor cells in the current malignant solid tumor cells falling into the blood before surgery and in the process of surgical tumor resection

Method used

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  • Inactivating system for circulating tumor cells in blood and application thereof
  • Inactivating system for circulating tumor cells in blood and application thereof
  • Inactivating system for circulating tumor cells in blood and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 A system for photochemically inactivating circulating tumor cells and / or tumor stem cells in blood or blood products

[0033] Such as figure 1The system for photochemically inactivating blood or blood products circulating tumor cells and / or tumor stem cells includes a blood input port (1), a blood output port (2), a light-transmitting tube (5), and a riboflavin adding device ( 3) and a visible light source (6) with a wavelength of 300-600nm, wherein the light-transmitting tube (5) is made of ethylene-vinyl acetate copolymer (EVA), and the light-transmitting tube (5) is connected to the blood input port (1) and the blood The output port (2) is connected, the light source (6) is arranged on one side of the light-transmitting tube (5), and the described riboflavin adding device (3) and anticoagulant adding device (4) are connected with the light-transmitting tube (5) In communication, a peristaltic pump (7) is also arranged on the light-transmitting tube. Its w...

Embodiment 2

[0034] Example 2 Riboflavin Photochemical Treatment of Human Colorectal Cancer HCT116 Cells

[0035] (1) Culture and expansion of human colorectal cancer HCT116 cells

[0036] Serum-free culture was adopted: the serum-free culture medium was DMEM / F12 culture medium added with cell growth factors (including 50ng / mLEGF, 10ng / mLNoggin and 50ng / mLLR-Spondin1). At the same time, make a 6-well plate with 1% agarose gel attachment surface for later use. Colorectal cancer HCT116 cells were taken to make a single cell suspension, resuspended in serum-free DMEM / F12 medium, stained with trypan blue and counted at 1×10 3 Individual / mL density was inoculated into the above-mentioned 6-well plate filled with SFM for routine culture. The HCT116 cell spheres cultured in SFM were washed with PBS and centrifuged to make a cell sphere suspension.

[0037] (2) Detection of the proportion of tumor stem cells in human colorectal cancer HCT116

[0038] HCT116 monolayer cells and tumor cell spher...

Embodiment 3

[0067] Example 3 Inactivation of Circulating Tumor Cells in Patient's Blood by Photochemical Method

[0068] The patient's blood is input from the blood input port (1) into the light-transmitting tube (5), and riboflavin is added to the blood through the riboflavin adding device (3), so that the concentration of riboflavin in the blood of the system is 40 μmol / L, the peristaltic pump (7) works to make the blood flow, and the light source (6) irradiates the blood flowing in the light-transmitting tube (5) for 45min by a wavelength of 300-600nm, and the irradiation dose is about 14J / cm The output port (2) outputs and re-inputs into the patient's body.

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Abstract

The invention discloses a photochemical inactivating system for circulating tumor cells and / or tumor stem cells in blood or blood products and application of riboflavin in preparation of an agent for inactivating the circulating tumor cells and / or the tumor stem cells in the blood or the blood products in a photochemical way. According to the system and application, the circulating tumor cells and / or the tumor stem cells in the peripheral blood are completely induced to be subjected to apoptosis to lose the proliferation ability, so that blood cells, coagulation function and immune function of a patient are maintained to the utmost extent.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to a system and application for inactivating circulating tumor cells and / or tumor stem cells by riboflavin photochemical method. Background technique [0002] Circulating tumor cells and / or tumor stem cells are a general term for various tumor cells that exist in peripheral blood. Due to spontaneous or therapeutic operations, they fall off from solid tumor lesions. Most of the circulating tumor cells and / or tumor stem cells enter the peripheral blood. Apoptosis or phagocytosis occurs, and a few can escape and develop into metastases, increasing the risk of recurrence in patients with malignant tumors. In particular, the problem of hematogenous dissemination of malignant solid tumor cells cannot be avoided during the shedding of malignant solid tumor cells into the blood before surgery and during tumor resection. [0003] At present, there have been reports on the treatment methods o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/36
CPCA61M1/3681A61M2210/12A61M2210/005
Inventor 于洋汪德清杨璐孙晓琳
Owner GENERAL HOSPITAL OF PLA
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