36 results about "Pseudomonas mendocina" patented technology

The invention belongs to the technical field of water environment biological treatment, in particular to a Pseudomonas mendocina strain CY004 for strongly degrade nitrite nitrogen, nitrate nitrogen and ammonia nitrogen, separation screening, safety measurement, a bacterial liquid preparation method and treatment application to domestic sewage and aquaculture sewage. The strain CY004 is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M 209197. By morphology observation, physiology and biochemistry analysis and 16S rDNA homology analysis, the strain CY004 is authenticated to be Pseudomonas mendocina ymp. The strain can quickly degrade the ammonia nitrogen, the nitrate nitrogen and the nitrite nitrogen in the water body, has no pathogenicity on animals of silver carp, sharksucker, shrimp, Eriocheir sinensis, white mouse and the like, and better degradation function on the nitrite and the ammonia nitrogen in the real sewage under normal temperature, degradation rate reaching 96.1 percent and 90 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the aquaculture sewage, degradation rate reaching 97.3 percent and 97.1 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the domestic sewage and better application prospect.

Bacterial strains transformed with the pcu genes are useful for the production of para-hydroxybenzoate (PHBA). Applicant has provided the p-cresol utilizing (pcu) and tmoX gene sequences from Pseudomonas mendocina KR-1, the proteins encoded by these sequences, recombinant plasmids containing such sequences, and bacterial host cells containing such plasmids or integrated sequences. Method for the use of these materials to produce PHBA are also disclosed.

The invention belongs to the technical field of microorganisms and discloses a microbial preparation for remediation of soil arsenic contamination. A fermented liquid of Bacillus licheniformis, a fermented liquid of Pseudomonas mendocina, a fermentation liquid of Nocardia corallina, a fermentation liquid of Arthrobacter crystallopoietes, a fermentation liquid of pseudomonas aeruginosa and a fermentation liquid of Clostridium papyrosolvens are uniformly mixed according to a certain volume ratio to form a mixed bacterial liquid, and the mixed bacterial liquid and a carrier are mixed according to a mass ratio of 1: 3 to form the microbial preparation. The microbial preparation realizes coordinate coexistence, has a reasonable formula and can effectively repair the arsenic-contaminated soil.

The invention discloses heterotrophic nitrification-aerobic denitrification pseudomonas mendocina as well as culture and application thereof. The strain is pseudomonas mendocina WZUF22 and is collected at the China General Microbiological Culture Collection Center with a registration number CGMCC No. 7523. The strain WZUF22 has proper pH (potential of Hydrogen) and wide temperature range during heterotrophic nitrification and aerobic denitrification, has high removal rate for NH4<+>-N, NO3<->-N and NO2<->-N and can provide a source for synchronous nitrification and denitrification.

The invention discloses a Pseudomonas strain YG8. The Pseudomonas strain YG8 (Pseudomonas mendocina YG8) is a heterotrophic nitrifying-aerobic denitrifying bacterium and is preserved in the Guangdong Provincial Microbial Culture Collection Center for a period of On March 21, 2018, the deposit number was GDMCC NO: 60340. The Pseudomonas strain YG8 has a high removal rate of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen at the same time, which can provide inoculum resources for the biological treatment of complex nitrogenous wastewater, and has great application value.

The invention discloses a Pseudomonas mendoza, which was preserved in the Guangdong Microbial Culture Collection Center on December 8, 2017, and the biological preservation number is GDMCC 60297. Correspondingly, the present invention also discloses the culture medium of Pseudomonas mendoza, including seed culture medium, fermentation medium and / or feed medium, wherein, fermentation medium comprises: starch 15-30g / L, corn steep liquor Dry powder 6-14g / L, dipotassium hydrogen phosphate 4-8g / L, ammonium sulfate 2-5g / L, magnesium sulfate heptahydrate 0.1-0.4g / L. Correspondingly, the invention also discloses a fermentation method of Pseudomonas mendoza and the application of Pseudomonas mendoza in sewage treatment. By adopting the invention, the low-cost and high-density fermentation of Pseudomonas mendoza is realized.

The invention discloses a mannuronic acid C-5 epimerase / alginate lyase coding gene, enzyme, preparation and application, and belongs to the field of genetic engineering. Specifically disclosed is an enzyme gene derived from Pseudomonas mendocina, which has two functions of epimerizing mannuronic acid C-5 and cracking alginate, and the preparation method and application of the enzyme, namely Using the technical method of genetic engineering, clone the gene of the enzyme into the expression vector of Escherichia coli to obtain the recombinant strain of Escherichia coli that can express the enzyme heterologously, and the enzyme prepared by the heterologous expression of the strain can efficiently transform β-D- Conversion of mannuronic acid (M) to α‑L‑guluronic acid (G) and cleavage of alginate to oligosaccharides. The bifunctional enzyme provided by the invention can be widely used in the fields of agriculture, food, feed additive, medicine and the like.

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.