36 results about "Pseudomonas mendocina" patented technology

The invention belongs to the technical field of microorganism denitrification, and particularly discloses a pseudomonas mendocina MKC-02 strain and the application of the pseudomonas mendocina MKC-02 strain to waste water denitrification. The strain is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) in November 14, 2016, and the preservation number is GDMCC No:60104. The strain is a heterotrophic nitrification-aerobic denitrification strain, simultaneous nitrification and denitrification is possible, and the strain has great application value in the sewage treatment aspect.

The invention belongs to the technical field of water environment biological treatment, in particular to a Pseudomonas mendocina strain CY004 for strongly degrade nitrite nitrogen, nitrate nitrogen and ammonia nitrogen, separation screening, safety measurement, a bacterial liquid preparation method and treatment application to domestic sewage and aquaculture sewage. The strain CY004 is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M 209197. By morphology observation, physiology and biochemistry analysis and 16S rDNA homology analysis, the strain CY004 is authenticated to be Pseudomonas mendocina ymp. The strain can quickly degrade the ammonia nitrogen, the nitrate nitrogen and the nitrite nitrogen in the water body, has no pathogenicity on animals of silver carp, sharksucker, shrimp, Eriocheir sinensis, white mouse and the like, and better degradation function on the nitrite and the ammonia nitrogen in the real sewage under normal temperature, degradation rate reaching 96.1 percent and 90 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the aquaculture sewage, degradation rate reaching 97.3 percent and 97.1 percent respectively on the nitrate nitrogen and the ammonia nitrogen in the domestic sewage and better application prospect.

The invention discloses a salt-tolerant Brevibacillus brevis and an application of complex microorganisms thereof in advanced treatment of high salt content degradation-resistant oil extraction sewage. The salt-tolerant Brevibacillus brevis is reserved in China General Microbiological Culture Collection Center, CGMCC for short, and the preservation number is CGMCC No.2148. The bacillus can bear high salinity which reaches 70000ppm, and has a wide applicable temperature range. The Brevibacillus brevis has a high COD degradation rate for petroleum hydrocarbon and salt-containing and oil-containing degradation-resistant sewage, and at 25-45 DEG C, the COD degradation rate for high salt content sewage (whose mineralization is higher than 30000ppm) reaches 60%. Brevibacillus brevis, Bacillus subtilis (registration number KC109003.1), Bacillus circulans Jordan (registration number JX893033.1), Bacillus amyloliquefaciens (registration number JX997921.1), Pseudomonas stutzeri (registration number KC113178.1) and Pseudomonas mendocina Marinobacter (registration number EU43329.1) compose complex microorganisms. A synergism is provided for high efficiency degradation of high salt content and oil containing degradation-resistant oil extraction sewage; the COD of incoming water is lower than 350ppm, and the COD of the discharged water after treatment is lower than 60ppm.

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.

Bacterial strains transformed with the pcu genes are useful for the production of para-hydroxybenzoate (PHBA). Applicant has provided the p-cresol utilizing (pcu) and tmoX gene sequences from Pseudomonas mendocina KR-1, the proteins encoded by these sequences, recombinant plasmids containing such sequences, and bacterial host cells containing such plasmids or integrated sequences. Method for the use of these materials to produce PHBA are also disclosed.

The invention provides a petroleum hydrocarbon degradation complex microbial inoculant and a preparation method thereof. The method comprises the following steps: 1) preparing a degradation bacterialsuspension, specifically, mycobacterium CSC-6, pseudomonas mendocina PM, pseudomonas stutzeri JN4, pseudomonas stutzeri JN5, bacillus licheniformis Y-1, bacillus pumilus L-2, acinetobacter XH-2 and acinetobacter junii BD are respectively cultured to a logarithmic growth phase, and then mixing each bacterial suspension to obtain the degradation bacterial suspension; 2) adding an adsorbent and sodium alginate to the degradation bacterial suspension obtained in step 1); 3) adding an intermedium obtained in step 2) to a granulation machine for granulation; 4) preparing a coating emulsion; 5) spraying the coating emulsion obtained in step 4) onto the surface of the granules obtained in step 3) to obtain the petroleum hydrocarbon degradation complex microbial inoculant. The complex microbial inoculant prepared by the method can effectively degrade the petroleum in the soil and achieve the purpose of treating petroleum-contaminated soil.

The invention belongs to the technical field of microorganisms and discloses a microbial preparation for remediation of soil arsenic contamination. A fermented liquid of Bacillus licheniformis, a fermented liquid of Pseudomonas mendocina, a fermentation liquid of Nocardia corallina, a fermentation liquid of Arthrobacter crystallopoietes, a fermentation liquid of pseudomonas aeruginosa and a fermentation liquid of Clostridium papyrosolvens are uniformly mixed according to a certain volume ratio to form a mixed bacterial liquid, and the mixed bacterial liquid and a carrier are mixed according to a mass ratio of 1: 3 to form the microbial preparation. The microbial preparation realizes coordinate coexistence, has a reasonable formula and can effectively repair the arsenic-contaminated soil.

The invention discloses heterotrophic nitrification-aerobic denitrification pseudomonas mendocina as well as culture and application thereof. The strain is pseudomonas mendocina WZUF22 and is collected at the China General Microbiological Culture Collection Center with a registration number CGMCC No. 7523. The strain WZUF22 has proper pH (potential of Hydrogen) and wide temperature range during heterotrophic nitrification and aerobic denitrification, has high removal rate for NH4<+>-N, NO3<->-N and NO2<->-N and can provide a source for synchronous nitrification and denitrification.

The invention discloses a Pseudomonas strain YG8. The Pseudomonas strain YG8 (Pseudomonas mendocina YG8) is a heterotrophic nitrifying-aerobic denitrifying bacterium and is preserved in the Guangdong Provincial Microbial Culture Collection Center for a period of On March 21, 2018, the deposit number was GDMCC NO: 60340. The Pseudomonas strain YG8 has a high removal rate of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen at the same time, which can provide inoculum resources for the biological treatment of complex nitrogenous wastewater, and has great application value.

The invention discloses a binary system, construction method and application of quorum sensing-aerobic denitrifying bacteria that strengthen biofilm denitrification under low temperature conditions, and belongs to the field of sewage treatment. The binary system includes aerobic denitrifying bacteria and aerobic denitrifying bacteria. The denitrifying bacterium is Pseudomonas mendocina ADBF, and the preservation number is CCTCC M 2019703 ADBF, which also includes quorum sensing bacteria with strong signal molecule production activity. The application includes the following process: First, isolate aerobic denitrifying bacteria with high group sensing ability and high nitrogen removal ability from an aerobic biofilm reactor that operates stably at low temperature; secondly, optimize its interaction with strong signal molecule production activity The optimal binary system composed of quorum-sensing bacteria; finally, the binary system was fixed on the carrier and added to the biofilm reactor to enhance low-temperature denitrification. The screening method in the present invention is convenient and quick, and the application of the prepared binary system can effectively improve the denitrification and denitrification efficiency of existing winter sewage treatment plants, and has high practical benefits for the improvement and transformation of winter sewage plants.

The invention discloses a Pseudomonas mendoza, which was preserved in the Guangdong Microbial Culture Collection Center on December 8, 2017, and the biological preservation number is GDMCC 60297. Correspondingly, the present invention also discloses the culture medium of Pseudomonas mendoza, including seed culture medium, fermentation medium and / or feed medium, wherein, fermentation medium comprises: starch 15-30g / L, corn steep liquor Dry powder 6-14g / L, dipotassium hydrogen phosphate 4-8g / L, ammonium sulfate 2-5g / L, magnesium sulfate heptahydrate 0.1-0.4g / L. Correspondingly, the invention also discloses a fermentation method of Pseudomonas mendoza and the application of Pseudomonas mendoza in sewage treatment. By adopting the invention, the low-cost and high-density fermentation of Pseudomonas mendoza is realized.

The invention discloses a mannuronic acid C-5 epimerase / alginate lyase coding gene, enzyme, preparation and application, and belongs to the field of genetic engineering. Specifically disclosed is an enzyme gene derived from Pseudomonas mendocina, which has two functions of epimerizing mannuronic acid C-5 and cracking alginate, and the preparation method and application of the enzyme, namely Using the technical method of genetic engineering, clone the gene of the enzyme into the expression vector of Escherichia coli to obtain the recombinant strain of Escherichia coli that can express the enzyme heterologously, and the enzyme prepared by the heterologous expression of the strain can efficiently transform β-D- Conversion of mannuronic acid (M) to α‑L‑guluronic acid (G) and cleavage of alginate to oligosaccharides. The bifunctional enzyme provided by the invention can be widely used in the fields of agriculture, food, feed additive, medicine and the like.

The invention discloses a magnetic whole-cell catalyst, a preparation method and a method for producing biodiesel. The preparation method includes the steps that firstly, pseudomonas mendocina cells are cultured and collected; secondly, SiO2-Fe3O4 nanoparticles are taken and added into a sodium alginate solution, the bacterial cells obtained in the first step are added after uniform stirring, and a mixed solution is obtained after uniform mixing; thirdly, the mixed solution is added into a sterile CaCl2 aqueous solution drop by drop under the stirring condition, still standing is carried out, magnetic microspheres are attracted out through a magnet and cleaned, and the magnetic whole-cell catalyst is obtained. The particle diameter of the prepared magnetic whole-cell catalyst is 2-6 mm, biological activity is high, and stability is good. In a biodiesel preparation reaction system, the magnetic whole-cell catalyst can be directly separated and extracted through the magnet and repeatedly used. The catalyst is easy to recycle, stable, easy to operate, moderate in condition, high in adaptability and safety and capable of reducing production cost of the biodiesel.

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.