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Microbe composite bacterial liquid and application thereof in preparing organic fertilizer

A technology of compound bacterial liquid and Clostridium, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve problems such as research gaps in decomposed inoculants, and achieve the effects of strengthening agricultural ecological protection, reducing production costs, and reducing usage.

Active Publication Date: 2012-06-13
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the current straw-decomposing bacterial agents, that is, cellulose-degrading bacterial agents, are developed for decomposing livestock and poultry manure or mixing them with straw and other materials under stacking conditions, while the decomposing bacterial agents developed for straw field conditions are currently research is almost blank

Method used

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  • Microbe composite bacterial liquid and application thereof in preparing organic fertilizer
  • Microbe composite bacterial liquid and application thereof in preparing organic fertilizer
  • Microbe composite bacterial liquid and application thereof in preparing organic fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: the preparation of composite bacterial liquid

[0016] 1. Fermentation of strains: The mixed cultured Clostridium thermostable, Clostridium succinate, Brevibacillus Potsdam and Paenibacillus were expanded by shaking table, seed tank fermentation and fermenter fermentation, the proportion of strains It is 8:2:10:6. The culture medium was prepared as follows: 0.5% peptone, 0.1% yeast extract, 1% cellulose (air-exploded straw), 0.5% sodium chloride, 0.5% calcium carbonate, pH 8.0, static culture at 50°C, making its DO 0.02 Microaerobic between -0.4, the straw can be degraded by more than 50% after 3 days of fermentation. The three strains of Bacillus subtilis, Pseudomonas mendoza and Providencia rettii were separately cultured on a slant, expanded on a shaker, fermented in a seed tank and fermented in a fermenter. Its culture medium formula is as follows: above-mentioned mixed flora culture 12000g is centrifuged to remove thalline and impurity sterilized cu...

Embodiment 2

[0017] Embodiment 2: the preparation of bacterial preparation product

[0018] The adsorbent of the present invention can be any adsorbent used in the preparation of organic fertilizers, preferably rice husk powder, which is pulverized from freshly processed dried rice husks. The fineness of the rice husk powder is more than 100 mesh, preferably 60-80 mesh. This is because rice husk powder has low moisture content and is easy to crush. After crushing, the fineness can reach more than 100 mesh, and its adsorption capacity is twice as high as that of peat, lignite and differentiated coal. It reaches 500-600 million / g, while peat, lignite and differentiated coal are only 200-300 million / g as carriers, which greatly improves the product quality; due to the low price of rice husk powder, it is used to produce compound microbial fertilizers and Bio-organic fertilizer can reduce the cost of using bacteria by more than 1 / 3.

[0019] The composite bacterial liquid prepared in Example...

Embodiment 3

[0020] Embodiment 3: prepare organic fertilizer with bacteria preparation product

[0021] 1. Sampling

[0022] Inoculate at a volume ratio of 5% (composite strains pre-cultivated for 3 days), and culture statically at 50°C. The treatment of composting is as follows: add the selected complex flora MCl at 1L / T, and add 1‰CaCO3 to adjust the pH during fermentation. After inoculation, add water according to the wet weight to control the moisture content of the straw at about 55-60%. Place it in a reaction pool with a height and width of 1.0m and a length of 10m for static composting. Cover with plastic film. Ventilate and supply oxygen by turning the compost from one end, turn over a distance of one meter to the end of the compost every day, and add the corresponding straw and seed liquid to the initial position of the compost, and turn the compost when the temperature of the center of the compost reaches 55°C sign. Turn the compost once a day, measure the temperature of the c...

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Abstract

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and specifically relates to a microbial compound bacterial liquid and its application in the preparation of organic fertilizers, that is, a bacterial liquid composed of a variety of non-antagonistic microorganisms, and its preparation method and application . Background technique [0002] At present, most of the microbial fertilizers in my country are common soil beneficial microorganisms, and the most widely used ones are mostly bacillus, including Bacillus megaterium, Bacillus subtilis and Bacillus coli. Bacillus megaterium can be used to produce phosphorus-solving and potassium-fixing fertilizers, which have a good effect on degrading organic phosphorus in the soil; Bacillus subtilis is mainly used to ferment and decompose various organic fertilizers (manure); Bacillus colloid is also called silicic acid Halobacteria can decompose potassium-containing minerals composed of glutam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C05F11/02C12R1/01C12R1/125C12R1/145C12R1/38
Inventor 张小霞温丹韩一凡黄志勇
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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