Microorganism strain for degrading petroleum hydrocarbon and combined repairing agent

A technology of microbial agent and combined repair, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of chemical repair that is difficult to fully meet the repair requirements, low comprehensive utilization, and unsatisfactory repair effects, etc., to achieve significant economic benefits. Benefits and social benefits, long-lasting environmental disturbances, effects of high utility value

Active Publication Date: 2018-06-08
NORTHWEST UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among these methods, physical restoration and chemical restoration are difficult to fully meet the restoration requirements, mainly manifested in problems such as secondary pollution, unsatisfactory restoration effect, high energy consumption, low degree of comprehensive utilization, and low resource recovery rate.

Method used

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  • Microorganism strain for degrading petroleum hydrocarbon and combined repairing agent
  • Microorganism strain for degrading petroleum hydrocarbon and combined repairing agent
  • Microorganism strain for degrading petroleum hydrocarbon and combined repairing agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 microbial bacterial agent

[0025] (1) Acquisition of microbial strains

[0026] The strain was derived from the oily soil near the Jiyuan Oilfield in Shaanxi. Using crude oil as the only carbon source, a strain with high oil degradation ability was finally isolated and screened by screening enrichment separation method. The identification results are shown in Table 1.

[0027] Table 1 strain identification results

[0028]

[0029] The strain obtained by the screening was deposited in the China Center for Type Culture Collection CCTCC on June 19, 2017. The preservation address is: Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M2017347.

[0030] (2) Inoculation and activation of microbial strains

[0031] Under sterile conditions, inoculate Pseudomonas mendocina PM (Pseudomonas mendocina PM) CCTCC NO: M2017347 into the Erlenmeyer flask containing nutrient b...

Embodiment 2

[0034] The remediation experiment of embodiment 2 microbial bacterial agents to oily soil

[0035] One, the laboratory degradation experiment of microbial bacterial agent of the present invention to n-alkane, horane, aromatic hydrocarbon:

[0036] The experiment was divided into two groups: blank oil sample group and PM oil sample experimental group.

[0037] The experimental process of the PM oil sample experimental group is as follows:

[0038] Under aseptic conditions, get the microbial bacterial agent 1.5ml that embodiment 1 prepares, insert the Erlenmeyer flask of 20ml inorganic salt medium, culture medium contains 10mg crude oil (inorganic salt medium formula: NH 4 NO 3 3g / L, K 2 HPO 4 1.6g / L, KH 2 PO 4 3g / L, trace element solution 6mL, distilled water 1000mL; trace element solution (g / L): MgSO 4 ·7H 2 O 0.15, FeSO 4 ·7H 2 O0.18, CuSO 4 0.15, anhydrous CaCl 2 0.02, Na 2 EDTA·2H 2 O 0.02, pH 7.1, 121°C high-pressure steam sterilization for 20min), 30°C s...

Embodiment 3

[0058] Example 3 Combined remediation experiment of microbial agent and oil-absorbing material on oily soil

[0059] Generally, oil-absorbing materials can be classified into inorganic mineral materials, synthetic organic materials, and natural organic materials. Inorganic mineral materials (such as clay, silica, vermiculite, etc.) have low oil absorption capacity and poor oil-water selectivity, which limits their wide application in the environment; synthetic organic materials (such as polyurethane) have high oil absorption properties, but there are There are many problems such as high synthesis cost, many by-products of oil absorption, and difficulty in handling after oil absorption. Compared with this, natural organic materials (such as corn) have great advantages, not only cheap and easy to obtain, but also can be completely removed from oily soil after oil absorption, with less environmental hazards; can be used in combination with other methods (such as microbial remedia...

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PUM

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Abstract

The invention provides a microorganism strain for degrading petroleum hydrocarbon. The microorganism strain is Pseudomonas mendocina PM, and the preservation number is CCTCC NO: M2017347. The invention further provides a combined oily soil repairing agent which comprises the microorganism strain as well as an oil absorption material. From a microbial flora for mainly screening and degrading polyaromatic hydrocarbon substances, a strain for effectively degrading petroleum hydrocarbon is prepared. In addition, from the angles of energy conservation and cheap material use, efficient oil absorption materials PGS and BMGS are prepared from corn straw in farmlands as a raw material and a mixture of styrene and butyl methacrylate-styrene as a grafting monomer. Degradation experiments show that the obtained modified corn straw BMGS is capable of rapidly degrading petroleum in oily soil together with microorganisms, and a very remarkable degradation effect can be achieved.

Description

technical field [0001] The invention provides a microbial strain capable of degrading petroleum hydrocarbons and a joint restoration agent, which can effectively degrade various petroleum hydrocarbons and can be used for joint treatment of oily soil. Background technique [0002] The methods of treating oily soil mainly include chemical leaching, heat treatment and bioremediation. Among these methods, physical restoration and chemical restoration are difficult to fully meet the restoration requirements, mainly manifested in problems such as secondary pollution, unsatisfactory restoration effect, high energy consumption, low comprehensive utilization degree, and low resource recovery rate. Bioremediation technology is considered to be a promising petroleum pollution remediation method that is environmentally friendly, has no secondary pollution, is highly efficient, and can completely degrade pollutants. The key to this technology is to use functional microorganisms to effici...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10B09C1/00C12R1/38
CPCB09C1/00B09C1/10C12N1/20C12N1/205C12R2001/38
Inventor 雒晓芳陈丽华杨成波王冬梅张璐李海玲
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
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