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Culture method and application of synchronous heterotrophic nitrification-aerobic denitrification pseudomonas mendocina

A technology of heterotrophic nitrification and cultivation method is applied in the cultivation field of simultaneous heterotrophic nitrification-aerobic denitrification of Pseudomonas mendoza, which can solve the problems of small number of viable bacteria, low fermentation efficiency and high cultivation cost, and achieves the The effect of low cost, simple cultivation method and high application value

Inactive Publication Date: 2021-01-05
南京师盛生态环境研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The fermentation efficiency of traditional synchronous heterotrophic nitrification and aerobic denitrification bacteria is low, the number of viable bacteria is small, and the cultivation cost is high

Method used

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  • Culture method and application of synchronous heterotrophic nitrification-aerobic denitrification pseudomonas mendocina
  • Culture method and application of synchronous heterotrophic nitrification-aerobic denitrification pseudomonas mendocina
  • Culture method and application of synchronous heterotrophic nitrification-aerobic denitrification pseudomonas mendocina

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Experimental program
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Effect test

Embodiment 1

[0031] The present invention provides a kind of culture method of synchronous heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza, wherein, the steps are:

[0032] A. Collection and preservation of strains:

[0033] a1. The source of Pseudomonas mendoza was isolated from the aerobic aeration tank of Nanjing Sembcorp Water Co., Ltd. in March 2020 by our laboratory. After morphological and PCR identification, it was confirmed as Mendoza by sequence comparison For Pseudomonas spp., Pseudomonas spp. were stored in LB solid plates, and stored in a refrigerator at 4°C for later use;

[0034] B. Strain culture:

[0035] b1. Pick the preserved Pseudomonas mendoza single bacteria and inoculate them in LB medium, the culture condition is 30°C, the dissolved oxygen is 3-4mg / l, cultivate for 18h, and centrifuge to obtain the precipitated bacteria. The bacterial cells are washed with sterile water to obtain a bacterial suspension;

[0036] b2. Inoculate the bacterial...

Embodiment 2

[0047] The invention also provides the application of synchronous heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza in sewage treatment.

[0048] The fermented liquid is applied to the domestic sewage treatment pumping station of Xuguang River in Liuhe District, Nanjing. The pumping station treats 600 cubic meters of sewage per day. The fermented liquid of the present invention is added to the aerobic tank and the denitrification tank, and the initial dosage is 100 L , Add 50L on the 5th day, and 50L on the 10th day, monitor the water quality changes in real time, and record the content of nitrite and nitrate.

[0049] The result looks like this:

[0050] The effect of Pseudomonas mendoza on total nitrogen in domestic sewage:

[0051]

[0052] Control group (no addition of Pseudomonas mendoza):

[0053]

[0054]

[0055] It can be concluded that after adding Pseudomonas mendoza, the total nitrogen removal rate has reached 94.1% on the 10th day,...

Embodiment 3

[0057] The invention also provides the application of synchronous heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza in sewage treatment.

[0058] Add the fermentation broth to a centrifuge tube, centrifuge at 6000r / min for 15min, discard the supernatant, and resuspend the bacteria with physiological saline to obtain a bacterial suspension.

[0059] The bacterial suspension is added to the membrane bioreactor at a volume ratio of 1%. The membrane bioreactor is mainly composed of a high-efficiency sedimentation zone and a membrane biological treatment zone. The influent is raw water from urban domestic sewage, and the treatment scale is 1m3 / d, the designed hydraulic retention time is 1h, the aeration method is intermittent aeration, the air-water ratio is 30:1, and the water temperature is 25-30°C.

[0060] Monitor the change of the total nitrogen removal rate of the membrane bioreactor before and after adding Pseudomonas mendoza:

[0061]

[0062] It ca...

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Abstract

The invention discloses a culture method and application of synchronous heterotrophic nitrification-aerobic denitrification pseudomonas mendocina. The culture method comprises the following steps: A,collection and storage of strains; and B, culture of the strains. The pseudomonas mendocina not only has excellent aerobic denitrification performance, but also has a heterotrophic nitrification effect, has higher application value in treatment of domestic sewage and other various polluted water bodies, can effectively reduce the content of nitrate nitrogen and total nitrogen in domestic sewage ofa membrane bioreactor when being applied to the membrane bioreactor, and has wide application prospects in development of denitrification microbial preparations or sewage treatment agents.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a cultivation method and application of synchronous heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza. Background technique [0002] Today, due to the impact of human activities, the ammonia nitrogen indicators in urban rivers and relatively open waters remain high. The resulting ecological and environmental problems have had a major impact on the human home environment and the image of the city. Microbial restoration of the water environment has become a hot research field at present. At present, the removal of ammonia nitrogen, nitrite, and nitrate in sewage water is gradually being upgraded to a new level along with the research on nitrifying bacteria and denitrifying bacteria. [0003] There are organic nitrogen, ammonia nitrogen, nitrate nitrogen and other forms of nitrogen in wastewater, of which ammonia nitrogen and organic nitrogen a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/30C12R1/38C02F101/16C02F101/38
CPCC02F3/302C02F3/34C02F2101/16C02F2101/163C02F2101/38C12N1/20
Inventor 朱辉王凯
Owner 南京师盛生态环境研究院有限公司
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