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Method for treating nitrite nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain

A nitrite nitrogen, aerobic denitrification technology, applied in the field of microorganisms, can solve the problems of restriction, high carbon-nitrogen ratio and poor high-concentration nitrogen resistance, and achieves excellent comprehensive performance and solves the effect of nitrite accumulation.

Inactive Publication Date: 2020-01-07
LONGYAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently disclosed HN-AD strains have poor tolerance to high carbon-to-nitrogen ratios and high concentrations of nitrogen, which restricts the practical application and promotion of heterotrophic nitrification-aerobic denitrification biological nitrogen removal technology in water pollution remediation.

Method used

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  • Method for treating nitrite nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain
  • Method for treating nitrite nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain
  • Method for treating nitrite nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] A kind of method utilizing above-mentioned heterotrophic nitrification-aerobic denitrification Pseudomonas bacterial strain LJ9 seed liquid to process the method for nitrite nitrogen waste water, comprises the following steps:

[0101] Inoculate the seed solution with 3% inoculation amount into the conical flask (specification 500ml) that is equipped with 200ml nitrosation medium respectively, the initial nitrite nitrogen concentration is 100mg L -1 , C / N=8, 30° C., 150 rpm, cultured on a shaker for 24 hours, and samples were taken every 6 hours for measurement. NaNO 2 In the medium as the sole nitrogen source, measure NO 3 - -N accumulation, pH value, NO 2 - -N, TN and COD content.

[0102] Aerobic denitrification performance research results

[0103] Strain LJ9 uses sodium succinate as carbon source, NaNO 2 As the only nitrogen source, the growth curve and nitrosation denitrification curve after 24 hours of cultivation are as follows: Image 6 Shown: Because o...

Embodiment 2

[0106] With sodium citrate as carbon source, the concentration of nitrite nitrogen or nitrate nitrogen is 100mg·L -1 , with a 3% inoculation amount, the seed solution was respectively inserted into the nitrite nitrogen medium or the nitrate nitrogen medium with C / N of 8, 25, 50, 75, 100, 120, 25 ° C, 180 rpm, shaking table After 24 hours of incubation, samples were taken to measure NO 2 - -N, NO 3 - -N and TN contents and their pH and OD 600 .

[0107] The effect of C / N on the performance of LJ9 aerobic denitrification

[0108] Strain LJ9 with NaNO 2 As the only nitrogen source, the initial NaNO 2 The concentration is 100mg·L -1 , cultivated for 24h, under different C / N conditions, the strain LJ9 has no effect on NO 2 - The case of -N degradation as Figure 7 Shown: With the increase of C / N, the removal rate of nitrite nitrogen, total nitrogen and OD 600 , pH showed a downward trend. OD of strain LJ9 when C / N is 8 600 Reaching 0.373, the removal rate of nitrite n...

Embodiment 3

[0111] With sodium citrate as the carbon source, C / N is 8, and the seed liquid is respectively inserted into the nitrite nitrogen concentration of 100, 150, 200, 300, 400, 500mg L with the inoculation amount of 3%. -1 In the nitrite nitrogen medium, 25 ℃, 180rpm, shaker culture for 24 hours, sampling NO 2 - -N and TN contents and their pH and OD 600 .

[0112] The effect of nitrite nitrogen content on the performance of LJ9 aerobic nitrosation

[0113] Strain LJ9 in different NO 2 - -N concentration, cultivated for 24h, NO 2 - The removal of -N is as follows Figure 8 Shown: With the increase of nitrite nitrogen concentration, OD 600 decreasing gradually. When the initial NO 2 - -N concentration is 100mg·L -1 and 150mg·L -1 , strain LJ9 had higher removal rates of nitrite nitrogen and total nitrogen, which were 54.64%, 45.84%, 48.46%, and 44.60%, respectively; the initial nitrite nitrogen concentration was 200mg·L -1 , the removal rate of nitrite nitrogen is low;...

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Abstract

The invention discloses a method for treating nitrite nitrogen wastewater by using a heterotrophic nitrification-aerobic denitrification pseudomonas strain, wherein the nitrate nitrogen wastewater contains organic acid sodium, and a C / N ratio of the nitrite nitrogen wastewater is 8-50. The method comprises: inoculating a heterotrophic nitrification-aerobic denitrification pseudomonas seed solutioninto nitrite nitrogen wastewater, and carrying out an oscillation reaction for 6-24 h at a temperature of 25-40 DEG C under a rotating speed of 90-180rpm so as to finish the treatment of the nitritenitrogen wastewater, wherein the strain is pseudomonas LJ9 and is preserved in the Guangdong culture collection center, has the preservation number of GDMCC NO:60339, has high C / N tolerance, and can tolerate higher-concentration nitrite nitrogen, so that the method has huge application value in the field of denitrification of high C / N and high nitrite nitrogen wastewater.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for treating nitrite nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification Pseudomonas strains. Background technique [0002] In recent years, the problem of water pollution has become increasingly serious. Nitrogen is an important pollution factor in water. Excessive nitrogen flowing into water can easily cause eutrophication of water, cause insufficient dissolved oxygen in water, lead to deterioration of water quality, and change water ecology. The environment, endangering the survival of aquatic organisms, is extremely harmful to the environment and human health. Nitrogen exists in water bodies in the form of organic nitrogen and inorganic nitrogen. The main source of organic nitrogen is domestic sewage, agricultural nitrogen fertilizer and industrial wastewater; Nitrate nitrogen, of which nitrate nitrogen can be converted into nitrite nit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/38C02F101/16
CPCC02F3/34C02F2101/166C12N1/20
Inventor 孙巍夏春雨洪维祎宋玉文杨雅玲苏全榕阙才英魏清斯易艳玲宋雅坚林美琴
Owner LONGYAN UNIV
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