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A method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification Pseudomonas strains

A technology of aerobic denitrification and ammonia nitrogen wastewater, applied in the field of microorganisms, can solve the problems of poor resistance to high carbon-nitrogen ratio and high-concentration nitrogen, and constraints, and achieve the effect of great application value

Active Publication Date: 2021-12-24
LONGYAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the currently disclosed HN-AD strains have poor tolerance to high carbon-to-nitrogen ratios and high concentrations of nitrogen, which restricts the practical application and promotion of heterotrophic nitrification-aerobic denitrification biological nitrogen removal technology in water pollution remediation.

Method used

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  • A method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification Pseudomonas strains
  • A method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification Pseudomonas strains
  • A method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification Pseudomonas strains

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] A kind of method utilizing above-mentioned heterotrophic nitrification-aerobic denitrification Pseudomonas bacterial strain LJ9 seed liquid to process the method for ammonia nitrogen wastewater, comprises the following steps:

[0107] With 3% inoculum size, the seed solution was respectively inserted into conical flasks (specification 500ml) equipped with 200ml heterotrophic nitrification medium, and the initial ammonia nitrogen concentration was 100mg L -1 , C / N is 8, pH is 7.5, 30°C, 150rpm, shaker culture for 48 hours, sampling every 6 hours, measure COD, NH 4 + -N, NO 2 - -N, NO 3 - -N and TN content, and their OD 600 and pH.

[0108] Research results of heterotrophic nitrification performance

[0109] Strain LJ9 uses sodium succinate as carbon source, NH 4 + -N is the only nitrogen source, and the growth and heterotrophic denitrification curve after 48 hours of culture is as follows Image 6 Shown: the strain LJ9, the growth rate of the 0-6h bacteria is s...

Embodiment 2

[0112] Ammonia nitrogen concentration 100mg·L -1 , change the carbon source content, and insert the seed liquid into the heterotrophic nitrification with C / N ratio of 4, 8, 12, 16, 20, 25, 30, 35, 50, 75, 100, 120 with 3% inoculation amount In the culture medium, sodium citrate was used as the carbon source, the pH was 7, 30°C, 150rpm, cultured on a shaker for 24 hours, and samples were taken to measure NH 4 + -N, TN content and its pH value and OD 600 .

[0113] The result is as Figure 7 Shown: strain LJ9 has a wide range of adaptability to C / N, and when the C / N is 8-120, the ammonia nitrogen removal rate of LJ9 can reach more than 80%. Compared with most heterotrophic nitrifying-aerobic denitrifying bacteria reported so far, LJ9 has high growth and denitrification resistance to C / N ratio.

[0114] High-concentration organic waste water that is easily biodegradable can be divided according to its nature and source: one type is industrial waste water that uses agricultur...

Embodiment 3

[0116] Add the seed solution to the ammonia nitrogen concentration of 100, 200, 300, 400, 500mg·L with an inoculation amount of 3%. -1 In the heterotrophic nitrification medium, sodium citrate was used as carbon source, C / N was 50, pH was 7, 25°C, 180rpm shaking table was continuously cultured for 7 days, samples were taken regularly every day, and NH 4 + -N content, NO 2 - -N and NO 3 - - Cumulative amount of N, its pH value and OD 600 . Selected biomass (OD 600 ) The highest day measured COD and TN content.

[0117] Effect of ammonia nitrogen concentration on the performance of LJ9 heterotrophic nitrification

[0118] Strain LJ9 was cultured under different initial ammonia nitrogen concentrations for 7 days, and its growth curve was as follows: Figure 8 Shown: initial ammonia nitrogen concentration 100-400mg·L -1 , OD of LJ9 600 With the increase of ammonia nitrogen content, the stagnant period of growth prolongs, when the concentration of ammonia nitrogen is 500mg...

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Abstract

The invention discloses a method for treating ammonia-nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification Pseudomonas strains. The ammonia-nitrogen wastewater contains sodium organic acid, and the C / N ratio is 8-120. The method comprises the following steps : Inoculate heterotrophic nitrification-aerobic denitrification Pseudomonas seed liquid into ammonia nitrogen wastewater, shake and react for 6h to 7d at a temperature of 25°C to 40°C and a speed of 90rpm to 180rpm to complete the treatment of ammonia nitrogen wastewater treatment; the bacterial strain is Pseudomonas LJ9, which is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 60339. The strain LJ9 has a high tolerance to high C / N and high ammonia nitrogen concentration wastewater and a high removal rate of ammonia nitrogen. Therefore, the present invention has great application value in removing ammonia nitrogen from wastewater with high carbon-to-nitrogen ratio and high concentration of ammonia nitrogen.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification Pseudomonas strains. Background technique [0002] In recent years, the problem of water pollution has become increasingly serious. Nitrogen is an important pollution factor in water. Excessive nitrogen flowing into water can easily cause eutrophication of water, cause insufficient dissolved oxygen in water, lead to deterioration of water quality, and change water ecology. The environment, endangering the survival of aquatic organisms, is extremely harmful to the environment and human health. Nitrogen exists in water bodies in the form of organic nitrogen and inorganic nitrogen. The main source of organic nitrogen is domestic sewage, agricultural nitrogen fertilizer and industrial wastewater; Nitrate nitrogen, of which nitrate nitrogen can be converted into nitrite nit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/20C02F101/16C12R1/38
CPCC02F3/34C12N1/20C02F2101/16
Inventor 孙巍夏春雨洪维祎宋玉文杨雅玲苏全榕阙才英魏清斯易艳玲宋雅坚林美琴
Owner LONGYAN UNIV
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