Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application and method of using pseudomonas mendocina NK-01 for production of brown alginate oligosaccharides

A technology for producing brown algae oligosaccharides and sapseudomonas, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of easy inactivation of enzymes, high price, low yield, etc., and achieves a simple process. , Short fermentation cycle, overcoming the cumbersome effect of separation and purification

Inactive Publication Date: 2009-10-14
NINGXIA WANSHENG BIO ENG
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main disadvantage of the chemical synthesis method is that multiple group protection and deprotection steps are required, the yield is low, and a large number of synthetic complex compounds cannot be obtained by conventional chemical methods, so there is no practical industrial value
To prepare fucoidan oligosaccharides by enzymatic method, most of them are separated and purified to obtain fucoidan oligosaccharide lyase first, and then a series of fucoidan oligosaccharide products are prepared. Synthesis is also more expensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application and method of using pseudomonas mendocina NK-01 for production of brown alginate oligosaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1, one-step fermentation to prepare fucoidan oligosaccharides (fermentation under low carbon and low nitrogen conditions)

[0022] The pre-cultivation is in the L-test tube, adding 5ml of LB medium by aseptic operation, inserting a single colony of NK-01 strain with a sterile toothpick, and incubating at 30°C and 120rpm for 12h. Inoculate 0.5 ml of the pre-culture into a 500 ml Erlenmeyer flask containing 100 ml of LB medium, and culture at 30° C. for 24 hours with shaking at 150 rpm. Sterile centrifuge at 6000g for 10min at 4°C, discard the supernatant, oscillate and mix with sterile phosphate buffer in a centrifuge tube, centrifuge again at 4°C, 6000g for 12min, discard the supernatant. The bacteria without LB medium were aseptically inserted into a 1-liter fermentation device with controllable oxygen stirring, which contained 1 liter of medium 1 (pH 7), and cultured at 30°C for 48 hours with aeration. After the fermentation, the cells were removed by centrif...

Embodiment 2

[0023] Example 2, two-step fermentation to prepare fucoidan oligosaccharides (fermentation under low carbon and nitrogen-free conditions)

[0024] The pre-cultivation is in the L-test tube, adding 5ml of LB medium by aseptic operation, inserting a single colony of NK-01 strain with a sterile toothpick, and incubating at 30°C and 120rpm for 12h. Inoculate 0.5 ml of the pre-culture into a 500 ml Erlenmeyer flask containing 100 ml of LB medium, and culture at 28° C. for 24 hours with shaking at 150 rpm. Sterile centrifuge at 6000g for 10min at 4°C, discard the supernatant, oscillate and mix with sterile phosphate buffer in a centrifuge tube, centrifuge again at 4°C, 6000g for 12min, discard the supernatant. The bacteria without LB medium were aseptically inserted into 10 bottles of 500ml Erlenmeyer flasks containing 100ml of medium 2 (pH 7), cultured at 30°C with shaking at 150rpm. Finish fermentation after 48 hours, following steps are with embodiment 1.

Embodiment 3

[0025] Example 3, Preparation of Fucoidan Oligosaccharides by One-step Fermentation (Fermentation under Low Carbon and Low Nitrogen Conditions)

[0026] The pre-cultivation is in the L-test tube, adding 5ml of LB medium by aseptic operation, inserting a single colony of NK-01 strain with a sterile toothpick, and incubating at 30°C and 120rpm for 12h. Inoculate 0.5 ml of the pre-culture into a 500 ml Erlenmeyer flask containing 100 ml of LB medium, and culture at 30° C. for 24 hours with shaking at 150 rpm. Sterile centrifuge at 6000g for 10min at 4°C, discard the supernatant, oscillate and mix with sterile phosphate buffer in a centrifuge tube, centrifuge again at 4°C, 6000g for 12min, discard the supernatant. The bacteria without LB medium were aseptically inserted into a 1-liter fermentation device with controllable oxygen stirring, which contained 1 liter of medium 3 (pH 6), and cultured at 28°C for 60 hours with aeration. Following steps are with embodiment 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
degree of polymerizationaaaaaaaaaa
Login to View More

Abstract

The invention discloses applications and a method of using pseudomonas mendocina NK-01 for the production of brown alginate oligosaccharides. The method comprises the following steps of: firstly carrying out activation culture on the bacteria in an L tube containing 5ml LB culture medium, then inoculating the activated microbial strain onto a fermentation culture medium for culture, centrifugally removing the bacteria after the completion of the fermentation, using 3 times of volume of anhydrous ethanol for carrying out sedimentation on supernatant liquid, using water for dissolving sediment after removing the supernatant liquid, then using qualitative filter paper for filtration, freezing and drying the filtrate, and obtaining the product of brown alginate oligosaccharides. The brown alginate oligosaccharides can be widely applied a plurality of fields such as foods, pharmaceutical, chemical and the like. The method overcomes the shortcomings that chemical method which is usually used for preparing the brown alginate oligosaccharides needs multi-group protection and the de-protection steps, the operation is complicated, the enzymatic method is easy to be inactivated and the price is expensive. The production method of the invention has simple process and short fermentation cycle; furthermore, the fermentation conditions and the process lead the large-scale industrial production to be possible.

Description

technical field [0001] The present invention relates to the use of Pseudomonas mendocina to produce polysaccharide biodegradable polymers, in particular to the use of Pseudomonas mendocina (Pseudomonas mendocina) NK-01 CCTCC M 208005 (referred to as Pseudomonas mendocina) NK-01) Use and method for producing fucoidan oligosaccharides. Background technique [0002] Fucoidan is an oligomer of fucoidan, which is a fucoidan with a degree of polymerization of less than 20. Fucoidan is a polysaccharide synthesized by seaweed and bacteria. In seaweed, fucoidan mainly acts as a skeleton, while in bacteria, fucoidan is a component of the cell wall, which prevents antibiotics from entering the cell, and some bacteria synthesize it. Fucoidan is secreted extracellularly. Sodium alginate or sodium alginate is a product in which the carboxyl hydrogen of uronic acid on the alginate polysaccharide chain is replaced by sodium, and they are also known as alginate. [0003] Fucoidan from sea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/38
Inventor 宋存江郭文斌王淑芳曹名锋耿伟涛
Owner NINGXIA WANSHENG BIO ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com