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Heterotrophic nitrification-aerobic denitrification strain for efficient denitrification and application of heterotrophic nitrification-aerobic denitrification strain

A technology of aerobic denitrification and heterotrophic nitrification, which is applied in the field of heterotrophic nitrification-aerobic denitrification bacterial strains for efficient denitrification, can solve the problem that nitrification and denitrification cannot be carried out simultaneously, the conditions of nitrification are harsh, and the effect of wastewater treatment is not good. To achieve good economic and environmental benefits, save equipment and investment costs, and achieve fast growth

Inactive Publication Date: 2021-07-27
FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Their discovery solves the shortcomings of traditional biological denitrification treatment such as long start-up time, poor stability, harsh conditions for nitrification, and inability to synchronize nitrification and denitrification. It is expected to overcome the contradiction between treatment efficiency and economic use of traditional treatment processes, and has good development prospects
However, as far as the current research situation is concerned, there are few strains of heterotrophic nitrifying and aerobic denitrifying bacteria, and the effect of treating wastewater is not good. Practicality becomes an urgent need

Method used

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  • Heterotrophic nitrification-aerobic denitrification strain for efficient denitrification and application of heterotrophic nitrification-aerobic denitrification strain
  • Heterotrophic nitrification-aerobic denitrification strain for efficient denitrification and application of heterotrophic nitrification-aerobic denitrification strain
  • Heterotrophic nitrification-aerobic denitrification strain for efficient denitrification and application of heterotrophic nitrification-aerobic denitrification strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the screening of heterotrophic nitrification-aerobic denitrification bacterial strain, concrete steps are as follows:

[0032] 1. Isolation and purification of bacteria

[0033] Put the sediment collected from the culture pond into the heterotrophic nitrification enrichment medium according to the volume ratio of 10% and cultivate it on a shaker at 30°C and 150r / min; every 48 hours, replace the fresh heterotrophic nitrification medium according to the volume ratio of 10%. The culture medium was enriched and cultured continuously for 20 days.

[0034] The above-mentioned domesticated microorganisms were inoculated into an aerobic denitrification enrichment medium for further screening, and the aerobic denitrification enrichment medium used potassium nitrate as the only nitrogen source.

[0035] Take 1mL of the sample that has passed the primary screening of the aerobic denitrification enrichment medium and spread it evenly on the solid GN chromogenic mediu...

Embodiment 2

[0042] Embodiment 2: Determination of ammonia nitrogen degradation ability of bacterial strain

[0043] Pick the colonies of the rescreened bacteria screened in Example 1 to the R2A liquid medium, cultivate them on an air-bath shaker at 200 rpm for 18 hours at 30°C, centrifuge at 4000r / min for 2min, collect the bacteria, and resuspend them with an equal amount of sterile water Bacteria are respectively inoculated into NM (heterotrophic nitrification enrichment medium) liquid medium according to the inoculation amount of 1% (volume ratio). 30°C, 200rpm shake flask culture, regular sampling to measure the optical density (OD600), total nitrogen (TN), ammonia nitrogen (NH 4 + -N), nitrate (NO 3 - -N) and nitrite (NO 2 - -N) concentration, draw the growth curve of thalline according to OD600 value. Such as figure 2 , 3 As shown, the strain grows rapidly, enters the logarithmic growth phase within 8 hours, enters the plateau phase within 48 hours, and reaches the highest O...

Embodiment 3

[0044] Embodiment 3: Determination of the aerobic denitrification ability of bacterial strain

[0045] Pick the colonies of re-screened bacteria screened in Example 1 to the R2A liquid medium, cultivate them in an air-bath shaker at 200 rpm for 18 hours at 30°C, centrifuge at 4000r / min for 2min, collect the bacteria, and weigh them with an equal amount of sterile water. Suspended bacteria were respectively inoculated into DMA and DMB liquid medium according to the inoculation amount of 1% (volume ratio). 30°C, 200rpm shake flask culture, regular sampling to measure the optical density (OD600), total nitrogen (TN), ammonia nitrogen (NH 4 + -N), nitrate (NO 3 - -N) and nitrite (NO 2 - -N) concentration, draw the growth curve of thalline according to OD600 value. Figure 5 In middle a, when nitrate was used as the only nitrogen source, the strain was cultured for 24 hours, and the total nitrogen removal rate was 92.8%; Figure 5 In b, when nitrite is the only nitrogen sour...

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Abstract

The invention provides a heterotrophic nitrification-aerobic denitrification strain for efficient denitrification, the strain is a Pseudomonas mendocina xh7, and the strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M2021275. The strain realizes synchronous removal of ammonia nitrogen, nitrite and nitrate nitrogen under an aerobic condition, and the removal efficiency is high; and nitrification and denitrification are coupled, so that the problems of slow proliferation of autotrophic nitrifying bacteria and long hydraulic retention time of the system can be effectively solved.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a highly efficient denitrification heterotrophic nitrification-aerobic denitrification bacterial strain and application thereof. Background technique [0002] In aquaculture, nitrogen pollution is one of the important factors leading to the deterioration of aquaculture water quality, which seriously threatens the survival of aquatic organisms. Microorganisms play an important role in nitrogen transformation. They can convert harmful nitrogen pollutants in water bodies into gaseous substances through ammonification, nitrification, and denitrification. Biological denitrification has attracted much attention because of its cost-effectiveness and environmental protection. Traditional biological denitrification usually includes two processes: the first step is nitrification, that is, nitrifying bacteria convert NH 4 + -N by NO 2 - -N converted to NO 3 - -...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
CPCC02F3/34C02F2101/16C02F2101/163C02F2101/166
Inventor 高娜梁阳阳杨坤李静方婷赵秀侠卢文轩洪大保
Owner FISHERIES RES INST ANHUI ACAD OF AGRI SCI
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