Petroleum hydrocarbon degradation complex microbial inoculant and preparation method thereof

Abstract

The invention provides a petroleum hydrocarbon degradation complex microbial inoculant and a preparation method thereof. The method comprises the following steps: 1) preparing a degradation bacterialsuspension, specifically, mycobacterium CSC-6, pseudomonas mendocina PM, pseudomonas stutzeri JN4, pseudomonas stutzeri JN5, bacillus licheniformis Y-1, bacillus pumilus L-2, acinetobacter XH-2 and acinetobacter junii BD are respectively cultured to a logarithmic growth phase, and then mixing each bacterial suspension to obtain the degradation bacterial suspension; 2) adding an adsorbent and sodium alginate to the degradation bacterial suspension obtained in step 1); 3) adding an intermedium obtained in step 2) to a granulation machine for granulation; 4) preparing a coating emulsion; 5) spraying the coating emulsion obtained in step 4) onto the surface of the granules obtained in step 3) to obtain the petroleum hydrocarbon degradation complex microbial inoculant. The complex microbial inoculant prepared by the method can effectively degrade the petroleum in the soil and achieve the purpose of treating petroleum-contaminated soil.

Description

Technical field [0001] The invention belongs to the technical field of soil improvement, and particularly relates to a petroleum hydrocarbon degradation composite bacterial agent and a preparation method thereof. Background technique [0002] Petroleum is one of the most important energy sources in modern society, and is called "the blood of industry" and "black gold". Petroleum is widely used in industrial fields such as the preparation of natural gas, gasoline, kerosene, volatile oil, lubricating oil, paraffin wax and asphalt. During the processing, use and transportation of crude oil, it is easy to cause spillage and leakage accidents, causing oil to enter the water body, soil and other environments and cause oil pollution. Petroleum is a complex mixture composed of a variety of hydrocarbons and a small amount of non-hydrocarbon compounds. It is difficult to be degraded in the environment. The polycyclic aromatic hydrocarbons in its components are a kind of persistent organic...

AI Technical Summary

Problems solved by technology

Physical and chemical methods have the problems of high treatment cost and incomplete treatment of pollutants, while bioremediation technology is an emerging technology developed in recent years to clean the environment. It uses the life activities of specific animals, plants, microorganisms, etc. to clean the environment. However, microorganisms play the largest role in the degradation of pollutants, and microbial degradation has been proven to be a very effective oil pollution removal technology, which has the advantages of cost-effectiveness, environmental friendliness, and no secondary pollution.

However, although the compound bacterial agents currently used for petroleum hydrocarbon degradation also contain some microorganisms that can degrade petroleum, the degradation effect of these composite bacterial agents is not satisfactory, which cannot improve the condition of the soil very well, and is not conducive to the growth of crops.

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