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Pseudomonas mendocina MKC-02 strain and application of pseudomonas mendocina MKC-02 strain to waste water denitrification

A technology of Pseudomonas sapseudomonas and MKC-02, applied in the field of microbial denitrification, can solve the problems of low, limited application and development, discontinuous efficiency of denitrification process, etc., and achieve the effect of good application prospects

Inactive Publication Date: 2017-03-22
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological denitrification technology separates the two processes of nitrification and denitrification, which leads to the discontinuity and low efficiency of the denitrification process, which seriously limits the application and development of the traditional biological denitrification technology.

Method used

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  • Pseudomonas mendocina MKC-02 strain and application of pseudomonas mendocina MKC-02 strain to waste water denitrification
  • Pseudomonas mendocina MKC-02 strain and application of pseudomonas mendocina MKC-02 strain to waste water denitrification
  • Pseudomonas mendocina MKC-02 strain and application of pseudomonas mendocina MKC-02 strain to waste water denitrification

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The screening of heterotrophic nitrification-aerobic denitrification bacterial strain, concrete steps are as follows:

[0030] 1. Acclimatization and enrichment: The experimental sludge comes from the activated sludge in the aerobic section of Guangzhou Datansha Wastewater Treatment Plant. Take 10 g of fully mixed activated sludge and place them in a tank containing 200 mL of nitrification culture solution and denitrification culture solution. 500 mL Erlenmeyer flask, placed in a shaker, set the temperature at 30°C, rotated at 120r / min, and acclimated to a period of 32 days. After each cycle, let the culture medium in the bottle stand still for a while, pour off the supernatant, and then re-add 250 mL of fresh culture medium for acclimatization. During this period, the removal of ammonia nitrogen and nitrate nitrogen was detected.

[0031] 2. Separation and purification: gradiently dilute 1mL of acclimatization solution to 10 with sterile water -1 、10 -3 、10 -5 10 ...

Embodiment 2

[0037] Growth and denitrification characteristics of Pseudomonas mendoza MKC-02 bacterial strain described in embodiment 1:

[0038] 1. After expanding the culture of the MKC-02 strain, resuspend the bacteria with sterile water (fixed volume to 1g / L), and then inoculate 10% of the inoculum into a 250mL Erlenmeyer flask containing 100mL of the above-mentioned heterotrophic nitrification medium , Shake well, culture at 30°C, 120r / min shaker for 72h, take samples regularly to measure the NH in the supernatant 4 + -N, NH 2 OH, NO 3 - -N, NO 2 - -N, COD, TN and OD 600 value, NO 3 - -N, NO 2 - -N, NH 2 OH, TN, COD and OD 600 value and NO 2 - -N, NO 3 - -N, NH 2 OH, TN, COD and OD 600 value.

[0039] see results Figure 4 ,according to Figure 4 The results show that when the strain uses ammonia nitrogen as the only nitrogen source, the MKC-02 strain enters the logarithmic phase after 12 hours of growth, and the strain enters the decay phase after a short stable ...

Embodiment 3

[0044] Example 3 Application of the FX02 strain described in Example 1 to strengthen denitrification

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Abstract

The invention belongs to the technical field of microorganism denitrification, and particularly discloses a pseudomonas mendocina MKC-02 strain and the application of the pseudomonas mendocina MKC-02 strain to waste water denitrification. The strain is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) in November 14, 2016, and the preservation number is GDMCC No:60104. The strain is a heterotrophic nitrification-aerobic denitrification strain, simultaneous nitrification and denitrification is possible, and the strain has great application value in the sewage treatment aspect.

Description

technical field [0001] The invention relates to the technical field of microbial denitrification, in particular to a Pseudomonas mendoza MKC-02 strain and its application in wastewater denitrification. Background technique [0002] In view of the deteriorating quality of water environment and the increasingly serious phenomenon of water eutrophication, efficient and rapid removal of nitrogen in water is an important topic in the field of water pollution prevention and control. The traditional biological denitrification technology separates the two processes of nitrification and denitrification, which leads to the discontinuity and low efficiency of the denitrification process, which seriously limits the application and development of the traditional biological denitrification technology. [0003] Studies have found that some heterotrophic nitrifying bacteria have the ability of aerobic denitrification at the same time, making it possible to carry out nitrification and denitr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/38
CPCC02F3/02C02F3/34C12N1/205C12R2001/38Y02W10/10
Inventor 曹刚张迪陈海升黄郑郑莫测辉
Owner JINAN UNIVERSITY
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