Mariculture tail water microbial purification preparation as well as preparation method and application thereof

A technology for microbial purification and mariculture, which is applied in the field of biofilm microbial purification preparations for mariculture tail water, can solve the problems of aquaculture water quality deterioration, flooding, death, etc., to optimize the community composition and structure, increase the total amount, and improve the hanging film efficiency effect

Active Publication Date: 2020-10-09
ZHEJIANG GONGSHANG UNIVERSITY
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  • Description
  • Claims
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Problems solved by technology

In addition, studies have shown that excessive content of organic matter and inorganic substances such as ammonia nitrogen and phosphorus in the water body will cause the deterioration of the water quality of the aquaculture, affect the normal growth of the aquaculture organisms, and even cause death or flooding. In severe cases, the quality of the aquaculture organisms and food safety will be affected

Method used

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  • Mariculture tail water microbial purification preparation as well as preparation method and application thereof
  • Mariculture tail water microbial purification preparation as well as preparation method and application thereof
  • Mariculture tail water microbial purification preparation as well as preparation method and application thereof

Examples

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Embodiment 1

[0024] This example proposes the preparation of a biofilm microbial purification preparation for mariculture tail water and its application in the purification of Penaeus vannamei mariculture tail water. The preparation specifically includes the following steps,

[0025] The cultured ppuI gene overexpression Pseudomonas putida bacteria liquid (5 × 10 8 CFU / mL) 2 copies, Pseudomonas mendoza (5×10 8 CFU / mL) 1 part, Pseudomonas stutzeri (5×10 8 CFU / mL) 1 part compounded into a mixed bacterial solution;

[0026] The mixed bacterial solution was centrifuged at 8000r / min for 10min, and the obtained bacterial sludge was uniformly dispersed in 10mL of 0.5mol / L mannitol and glucose compound protective agent solution to make a bacterial suspension, and pre-cooled at 4°C for 40min. Put it into liquid nitrogen for pre-freezing for 2 hours, transfer it to a freeze dryer, and carry out vacuum drying to obtain a freeze-dried powder of mixed bacteria, which is sealed and stored in a refrig...

Embodiment 2

[0032] In this embodiment, a preparation method of microbial purification preparation and its application in the purification of large yellow croaker mariculture tail water are proposed, wherein the preparation method is as follows:

[0033] The cultured ppuI gene overexpression Pseudomonas putida bacteria liquid (6 × 10 8 CFU / mL) 3 copies, Pseudomonas mendoza (6×10 8 CFU / mL) 2 copies, Pseudomonas stutzeri (6×10 8 CFU / mL) 2 parts compounded into a mixed bacterial solution;

[0034]Centrifuge the mixed bacterial solution at a speed of 10000r / min for 10min, and the obtained bacterial sludge is evenly dispersed in 10mL of 0.5mol / L mannitol and glucose compound protective agent solution to make a bacterial suspension, and pre-cooled at 4°C for 40min. Put it into liquid nitrogen for pre-freezing for 2 hours, transfer it to a freeze dryer, and carry out vacuum drying to obtain a freeze-dried powder of mixed bacteria, which is sealed and stored in a refrigerator at 4°C;

[0035] R...

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Abstract

The invention discloses a mariculture tail water microbial purification preparation as well as a preparation method and application thereof. Functional microorganisms of the biological membrane microbial purification preparation comprise pseudomonas putida overexpressed by ppuI genes, pseudomonas mendocina and pseudomonas stutzeri. The mariculture tail water microbial purification preparation hasthe beneficial effects that the ppuI genes for guiding biosynthesis of signal molecules 3-oxo-C10 and 3-oxo-C12 are overexpressed in the pseudomonas putida, so that the content of the signal moleculesand the biofilm culturing efficiency of an attachment base are effectively improved, and the total amount of biological films is increased; meanwhile, the community composition and structure of the biological film are optimized, the purification efficiency of pollutants such as ammonia nitrogen, nitrite and phosphorus in the mariculture tail water is remarkably improved, and the economic cost isreduced.

Description

technical field [0001] The invention relates to the technical field of aquaculture and environmental protection, in particular to a biofilm microbial purification preparation for marine aquaculture tail water, a preparation method and application thereof. Background technique [0002] In recent years, with the increase in demand for seafood and the depletion of fishery resources, the scale of mariculture has continued to expand. In high-density mariculture, a large amount of fertilization and baiting are required. Residual bait, excrement of farmed animals and biological debris can easily cause ammonia nitrogen, nitrite, phosphorus and other pollutants in the aquaculture system to exceed the standard. These aquaculture tail waters are directly discharged into adjacent waters, leading to a series of problems such as the spread of pollution, the deterioration of the water environment, and the eutrophication of the waters. Mariculture has become one of the important sources of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N1/20C12N1/04C12N15/78C12N15/31C02F3/34C12R1/40C12R1/38C02F103/20
CPCC12N1/20C12N1/04C12N15/78C07K14/21C02F3/341C02F2103/20
Inventor 傅玲琳王彦波王飞飞周瑾茹周志刚刘长军伊祥华
Owner ZHEJIANG GONGSHANG UNIVERSITY
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