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Heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza and its cultivation and application

An aerobic denitrification and denitrification culture medium technology is applied in the field of heterotrophic nitrification-aerobic denitrification of Pseudomonas mendoza, which can solve the problems of weak ability to remove ammonium nitrogen and the like, achieve high removal rate, Good effect of reducing COD of wastewater and denitrification

Inactive Publication Date: 2015-08-05
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Another example is that Jibin Zhang et al. isolated a strain of Pseudomonas stutzeri from pig manure sewage ( Pseudomonas stutzeri YZN-001), at 10°C, 20°C, 30°C and 37°C, NH 4 + The nitrification rates of -N were about 1.48, 4.20, 5.53 and 5.59 mg·L, respectively -1 h -1 NH 4 + –N; the nitration rates of nitric acid and nitrous acid were 11.46 mg·L at 30°C -1 h -1 NO 2 - -N and 10.99 mg·L -1 h -1 NO 3 - -N (Bioresource Technology, 2011, 102 : 9866–9869), its ability to remove ammonium nitrogen is weak

Method used

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  • Heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza and its cultivation and application
  • Heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza and its cultivation and application
  • Heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza and its cultivation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Isolation of Pseudomonas mendoza strains of heterotrophic nitrification-aerobic denitrification

[0034] The sample is activated sludge from Wenzhou Xipian Sewage Treatment Plant. A certain amount of activated sludge was inoculated into the enrichment medium (NH 4 Cl 1g, sodium citrate 5g, MgSO 4 ·7H 2 O 0.1g, K 2 HPO 4 0.5g, NaCl 0.2g, MnSO 4 4H 2 O 0.02g, FeSO 4 0.02g, H 2 O 1000 ml, pH 7.0) at 30°C at 160-180 rpm for 3-4 days for enrichment culture, after bacteria grow, transfer to a new enrichment medium to continue enrichment culture, and repeat this 4-5 times. Then spread the appropriately diluted bacterial solution on the separation medium (agar 18g·L -1 , other components are the same as the enrichment medium) and cultured at 30°C for 48 h, pick a single colony and transfer it to a new isolation medium for streaking and purification, until it is a pure culture by microscopic examination, and then transfer to the preservation medium (beef Cr...

Embodiment 2

[0042] Example 2: Identification of Heterotrophic Nitrification-Aerobic Denitrification Strains

[0043] The colonies of the strain WZUF22 were round, translucent, with rough surfaces, irregular edges, light yellow colonies after being cultured in the isolation medium for 48 hours. Lange-negative, unipolar flagella.

[0044] 16S rDNA was amplified using bacterial genomic DNA as a template, using a pair of universal primers: upstream primer (P1): 5'-AGAGTTTGATCCTGGTCAGAACGAACGCT-3', downstream primer (P6): 5'-TACGGCTACCTTGTTACGACTTCACCCC-3', and purification of PCR products And the sequencing was completed by Shanghai Bioengineering Co., Ltd., and the sequencing results were compared and analyzed by GeenBank Blast. with Pseudomonas in GeenBank ( Pseudomonas sp .) The 16SrDNA sequence has a high homology, and P. mendocina The homology is 99.4%. Using MEGA4.1 software, the phylogenetic tree of the 16S rDNA sequence of strain WZUF22 and related species was displayed by the a...

Embodiment 3

[0046] Example 3: Strain WZUF22 removes NH 4 + -N and NO 3 - -N characteristics

[0047] Using the single factor test method, the research strain WZUF22 was subjected to heterotrophic nitrification to remove NH 4 + -N and aerobic denitrification to remove NO 3 - -N characteristics.

[0048] The experimental process is as follows: the preserved strain WZUF22 (2.0ml frozen tube thawed bacterial solution) was inoculated into 200ml LB medium (10g beef extract, 10g peptone, 5g NaCl, 18g agar, H 2 O 1000 ml, pH 7.0) in a 500ml Erlenmeyer flask, cultured at 30°C, 150rpm for 24 h, centrifuged at 8000rpm for 10min to obtain bacterial cells, washed twice with sterile water to prepare OD 680 0.900~1.000 bacterial suspension;

[0049] Bacteria suspension was transferred to 100ml nitrification medium (NH 4 Cl, carbon source, MgSO 4 ·7H 2 O, K 2 HPO 4 , NaCl, MnSO 4 4H 2 O, FeSO 4 , H 2 O, pH 4~10.5) or denitrification medium (carbon source, KNO 3 , K 2 HPO 4 , FeSO ...

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Abstract

The invention discloses heterotrophic nitrification-aerobic denitrification pseudomonas mendocina as well as culture and application thereof. The strain is pseudomonas mendocina WZUF22 and is collected at the China General Microbiological Culture Collection Center with a registration number CGMCC No. 7523. The strain WZUF22 has proper pH (potential of Hydrogen) and wide temperature range during heterotrophic nitrification and aerobic denitrification, has high removal rate for NH4<+>-N, NO3<->-N and NO2<->-N and can provide a source for synchronous nitrification and denitrification.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a heterotrophic nitrification-aerobic denitrification Pseudomonas mendoza strain and its cultivation and application. Background technique [0002] The pollution caused by nitrogen to the environment has become increasingly prominent in recent years, and its harmfulness has been increasingly recognized and valued by people. For example, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen may be transformed into carcinogenic, mutagenic and teratogenic nitrosamines; another example is the inflow of nitrogen into water bodies to cause eutrophication of water bodies, causing deterioration of water quality and even lake degradation. Biological nitrogen removal has the advantages of good treatment effect, stable and reliable treatment process, and convenient operation and management, so it has been widely used. [0003] Traditional biological denitrification...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/38
CPCY02W10/10
Inventor 周茂洪赵肖为叶海仁蒋张亮
Owner WENZHOU UNIVERSITY
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