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Method for strengthening n-hexane biodegradation by means of nano-magnetic silicone oil

A nano-magnetism, n-hexane technology, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of low surface tension, easy foaming, easy emulsification, etc., and achieve the effect of efficient degradation

Active Publication Date: 2017-02-01
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the addition of organic phases such as silicone oil and hexadecane has significantly improved the waste gas treatment effect and impact load resistance of the two-phase reactor, the liquid NAP has obvious defects: the surface tension is small and Easy to produce foam, easy to emulsify; difficult to recycle, high economic cost
[0007] The present invention uses nano-magnetic silicone oil as NAP to strengthen the biological purification of n-hexane waste gas and waste water, so far there is no literature report

Method used

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  • Method for strengthening n-hexane biodegradation by means of nano-magnetic silicone oil
  • Method for strengthening n-hexane biodegradation by means of nano-magnetic silicone oil
  • Method for strengthening n-hexane biodegradation by means of nano-magnetic silicone oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 nanometer magnetic silicone oil

[0041] (1) Nano Fe 3 o 4 Preparation: 1.340g FeSO 4 ·7H 2 O and 1.304 g FeCl 3 Put into 450ml deionized water (total concentration of iron salt is 0.135mol / L, Fe 2+ :Fe 3+ The ratio of the amount of the substance is 1.65:1), under the protection of nitrogen, add 45ml of ammonia water with a concentration of 0.25mol / L dropwise, and mechanically stir at a rate of 800r / min at a constant temperature of 27°C, and after aging for 1 hour , washed with deionized water to obtain nano-Fe 3 o 4 1g, stored in pure alcohol for later use. The resulting nano-Fe 3 o 4 The particle size is about 13nm.

[0042] (2) Nano Fe 3 o 4 The coating process of the silane coupling agent: get the nano-Fe prepared by 1g step (1) 3 o 4 Put 0.6mL of silane coupling agent KH570 in 162mL of 92.5% ethanol aqueous solution, under nitrogen protection, mechanically stir at 300r / min at a constant temperature of 70°C for 2 hours, then wash with abs...

Embodiment 2

[0045] Embodiment 2 Pseudomonas mendoza NX-1 bacterial liquid

[0046] 1) Incline cultivation: inoculate Pseudomonas mendocina (Pseudomonas mendocina) NX-1 (preservation number CCTCCNO: M2015114, disclosed in patent application CN105087440A) in R 2 A solid slant culture medium, cultured at 30-32°C for 36-48 hours to obtain slant bacteria, the R 2 The final concentration of A solid slant medium is composed of: yeast powder 0.50g / L, casein 0.50g / L, soluble starch 0.50g / L, MgCl 2 ·7H 2 O 0.05g / L, tryptone 0.50g / L, glucose 0.50g / L, sodium pyruvate 0.30g / L, KH 2 PO 4 0.45g / L, agar 15~18g / L, pH 7.2, solvent is water.

[0047]2) Seed culture: use an inoculation loop to pick the slant cells obtained in step (1) and inoculate them into the inorganic salt medium containing n-hexane with a final concentration of 207 mg / L, culture at 30-32°C for 36-38 hours, and obtain the wet cell concentration It is 19.4mg / L seed solution.

Embodiment 3

[0048] Biotoxicity and biodegradability of embodiment 3 nanometer magnetic silicone oil

[0049] 1) Biotoxicity test of nanometer magnetic silicone oil: add 43mL inorganic salt culture medium, 2mL Pseudomonas mendoza NX-1 seed liquid (prepared in embodiment 2) and 5mL nanometer magnetic silicone oil (embodiment 1) in 250mL sealed bottle Preparation, Fe 3 o 4 Content is 33.3g / L) and the glucose that adds final concentration is 1g / L. Each sealed bottle is equipped with 3 parallel experimental groups and a blank control group (an equal volume of inorganic salt instead of nano-magnetic silicone oil). Put the above sealed bottle into a shaker with a temperature of 30°C and a rotation speed of 160r / min to incubate and shake for 60 hours, and measure the CO in the headspace of the sealed bottle by gas chromatography every 24 hours. 2 content. If the experimental group CO 2 A substance produced in an amount five times lower than that of the control is considered biotoxic. Studie...

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Abstract

The invention discloses a method for strengthening n-hexane biodegradation by means of nano-magnetic silicone oil. The method comprises the steps that the nano-magnetic silicone oil is prepared in the mode that nanometer Fe3O4 is coated with a silane coupling agent, and a two-phase distribution reaction system is formed by taking a wet cell obtained by conducting fermenting culture on Pseudomonas mendocina NX-1 as a catalyst and taking n-hexane as a substrate in an inorganic salt culture medium for degradation. Compared with a single-phase system, the two-phase distribution reaction system has the advantage that the n-hexane removing rate is increased by 10 times; compared with common silicone oil, the nano-magnetic silicone oil can be conveniently recycled and reutilized, the operation cost is reduced, and the removing rate is increased by 3 times or above.

Description

[0001] (1) Technical field [0002] The invention relates to the application of a nano-magnetic silicone oil as a non-aqueous phase system (NAP) in a two-phase partition biological reaction system (TPPB system) in strengthening microorganisms to degrade n-hexane. [0003] (2) Background technology [0004] Methyl n-hexane (n-hexane, C 6 h 6 ) is a colorless liquid with low toxicity and a faint special smell. n-Hexane is highly fat soluble and easily accumulates in the organism. Because it is volatile at room temperature, it can cause chronic poisoning with sensorimotor polyneuropathy as the main clinical manifestation after entering the human body. When the concentration exceeds a certain level, respiratory tract irritation symptoms, anesthesia symptoms, and acute poisoning symptoms of respiratory failure and suffocation will appear. Chronic poisoning such as peripheral neuropathy, heart damage, and visual impairment will occur if exposed to low-concentration n-hexane for a...

Claims

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Application Information

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IPC IPC(8): C02F3/34B01D53/84B82Y40/00C12P1/04B82Y30/00C12R1/38C02F101/32
CPCB01D53/84B01D2257/7022B01D2257/708B82Y30/00C02F3/34C02F2101/32C02F2203/004C02F2305/08C12P1/04C12N1/205C12R2001/38Y02A50/20
Inventor 陈东之陈建孟雷昱叶杰旭褚其英
Owner ZHEJIANG UNIV OF TECH
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