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A binary system, construction method and application of quorum-sensing-aerobic denitrifying bacteria for enhanced biofilm denitrification under low temperature conditions

An aerobic denitrifying bacteria and quorum sensing technology, applied in the field of binary system of quorum sensing-aerobic denitrifying bacteria, can solve the problems of high cost of signal molecules and easy to be degraded by microorganisms, so as to improve the overall operation effect and reverse the The effect of improving nitrification efficiency and film forming speed

Active Publication Date: 2021-12-17
NANJING UNIV
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  • Abstract
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Problems solved by technology

[0011] Aiming at the technical problems of limited enhancement effect of adding existing aerobic denitrifying bacteria alone, high cost of adding signal molecules and being easily degraded by microorganisms, the present invention provides a quorum sensing-aerobic denitrification method for enhancing biofilm denitrification under low temperature conditions The binary system, construction method and application of denitrifying bacteria. By screening aerobic denitrifying bacteria with high quorum sensing ability and quorum sensing bacteria to form a binary system, not only can the signal molecules secreted by quorum sensing bacteria be used to improve aerobic reaction The denitrification effect of nitrifying bacteria can also realize the synergistic denitrification of aerobic denitrifying bacteria and quorum sensing bacteria, effectively improving the treatment effect of nitrogen-containing wastewater at low temperature, and has strong economic benefits and practical value

Method used

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  • A binary system, construction method and application of quorum-sensing-aerobic denitrifying bacteria for enhanced biofilm denitrification under low temperature conditions
  • A binary system, construction method and application of quorum-sensing-aerobic denitrifying bacteria for enhanced biofilm denitrification under low temperature conditions
  • A binary system, construction method and application of quorum-sensing-aerobic denitrifying bacteria for enhanced biofilm denitrification under low temperature conditions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Construction of Quorum Sensing-Aerobic Denitrifying Bacteria Binary System

[0057] (1) The filler was taken out from the aerobic biofilm reactor operated stably at 8°C in the Laboratory of Water Treatment and Water Environment Restoration Biotechnology Team of Nanjing University, and the biofilm was separated and placed in the denitrification medium to enrich the aerobic biofilm. Denitrifying bacteria, after repeated isolation and purification, the concentration and type of quorum-sensing N-acyl homoserine lactone signal molecules (AHLs), the concentration of extracellular polymers (EPS) and the concentration of aerobic denitrifying bacteria were tested. Composition, growth amount and denitrification rate, and then according to the production or degradation characteristics of the signal molecules (AHLs), the strains with high quorum sensing ability were selected; according to the Pearson correlation analysis, the signal molecules and film-forming ability, Quorum-sensin...

Embodiment 2

[0064] Identification of aerobic denitrifying bacteria and quorum sensing-characteristics of aerobic denitrifying bacteria binary system

[0065] (1) The DNA of the aerobic denitrifying bacteria obtained in accordance with all the screening steps in Example 1 was extracted, amplified by PCR with bacterial universal primers and sequenced. The results obtained from the sequencing were compared with the 16S rDNA gene sequence in the GenBank database using NCBI's BLAST tool for homology comparison search. The results showed that the optimized screened aerobic denitrifier had 99.14% homology with Pseudomonas mendocina, so it was named Pseudomonas mendocina ABDF. The electron microscope pictures taken after its pure culture for 24 hours are as follows: Figure 5 As shown, the bacteria were uniform in shape, rod-shaped and free of bacterial contamination.

[0066] (2) Quorum sensing-aerobic denitrifying bacteria binary system characteristic detection

[0067] Take 0.5 mL of the susp...

Embodiment 3

[0072] A method for strengthening biofilm method for treating nitrogen-containing wastewater under low temperature conditions, comprising the following processes:

[0073] The binary system in Example 2 was inoculated into the LB medium containing the biological carrier and cultured for 24 hours to fix the bacteria. The inoculation amount of the binary system is 1% (v / v), and the ratio between the quorum-sensing-aerobic denitrifying bacteria and the quorum-sensing bacteria S.rubra is 1:1 by volume. The carrier immobilized with the binary system is added to the biofilm reactor inoculated with activated sludge in a certain proportion, and the free bacteria obtained after centrifuging the LB are also put into the reactor. The biological carrier of the fixed binary system accounts for 10% (v / v) of the total number of carriers in the biofilm reactor, and the total volume of the carrier is 35% (v / v) of the effective volume of the reactor. The reactor is placed in a constant tempera...

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Abstract

The invention discloses a binary system, construction method and application of quorum sensing-aerobic denitrifying bacteria that strengthen biofilm denitrification under low temperature conditions, and belongs to the field of sewage treatment. The binary system includes aerobic denitrifying bacteria and aerobic denitrifying bacteria. The denitrifying bacterium is Pseudomonas mendocina ADBF, and the preservation number is CCTCC M 2019703 ADBF, which also includes quorum sensing bacteria with strong signal molecule production activity. The application includes the following process: First, isolate aerobic denitrifying bacteria with high group sensing ability and high nitrogen removal ability from an aerobic biofilm reactor that operates stably at low temperature; secondly, optimize its interaction with strong signal molecule production activity The optimal binary system composed of quorum-sensing bacteria; finally, the binary system was fixed on the carrier and added to the biofilm reactor to enhance low-temperature denitrification. The screening method in the present invention is convenient and quick, and the application of the prepared binary system can effectively improve the denitrification and denitrification efficiency of existing winter sewage treatment plants, and has high practical benefits for the improvement and transformation of winter sewage plants.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and more specifically relates to a binary system, construction method and application of quorum sensing-aerobic denitrifying bacteria that strengthen biofilm denitrification under low temperature conditions. Background technique [0002] The winter climate in northern China is cold, which can easily affect the biological denitrification effect of sewage treatment plants, making the effluent water quality not meet the first-class A standard. Therefore, how to find an effective strengthening method to improve the denitrification effect under low temperature conditions is one of the current problems, and the research results are of great significance for improving the effect of sewage treatment in winter. [0003] The biofilm method has the characteristics of large biomass, long microbial residence time, and the ability to inhabit nitrifying bacteria and denitrifying bacteria with slow proliferation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/12C02F101/16
CPCC02F3/34C02F3/1268C02F2101/16Y02W10/10
Inventor 丁丽丽王旭朱颖楠任洪强
Owner NANJING UNIV
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