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A kind of microbial preparation for remediating soil arsenic pollution

A microbial preparation and soil remediation technology, applied in the field of microorganisms, to achieve the effects of broad application prospects, improved hydrophobicity, and increased adsorption sites

Active Publication Date: 2020-04-24
SHENZHEN KUAKE BIOSCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of biological remediation of soil arsenic pollution in the prior art, the present invention provides a microbial preparation for remediation of soil arsenic pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A microbial preparation for remediating soil arsenic pollution, which is prepared according to the following method:

[0028] Add the same weight of hydrochloric acid solution with a mass fraction of 12% to attapulgite, stir at 300rpm for 20min, then add sodium dodecylsulfonate and 5% by weight of attapulgite, 200rpm Stir for 60 minutes, then centrifuge to collect the precipitate; crush the wheat straw with a pulverizer, and then pass it through a 100-mesh sieve to obtain straw powder; mix and stir the above-mentioned precipitate and straw powder according to the mass ratio of 2:1 to obtain the carrier;

[0029] Bacillus licheniformis, Pseudomonas mendoza, Nocardia corynus, Arthrobacter aeruginosa, Pseudomonas aeruginosa, and Clostridium aeruginosa were cultured on slant, shake flask seed and fermenter respectively to obtain fermentation liquid; the concentrations of the above six fermentation liquids were all controlled at 1×10 10 cfu / ml;

[0030] By Bacillus licheni...

Embodiment 2

[0038] A microbial preparation for remediating soil arsenic pollution, which is prepared according to the following method:

[0039] Add the same weight of hydrochloric acid solution with a mass fraction of 12% to attapulgite, stir at 300rpm for 20min, then add sodium dodecylsulfonate and 5% by weight of attapulgite, 200rpm Stir for 60 minutes, then centrifuge to collect the precipitate; crush the wheat straw with a pulverizer, and then pass it through a 100-mesh sieve to obtain straw powder; mix and stir the above-mentioned precipitate and straw powder according to the mass ratio of 2:1 to obtain the carrier;

[0040] Bacillus licheniformis, Pseudomonas mendoza, Nocardia corynus, Arthrobacter aeruginosa, Pseudomonas aeruginosa, and Clostridium aeruginosa were cultured on slant, shake flask seed and fermenter respectively to obtain fermentation liquid; the concentrations of the above six fermentation liquids were all controlled at 1×10 10 cfu / ml;

[0041]With Bacillus lichen...

Embodiment 3

[0049] The microbial preparation of the present invention is to the removal test of arsenic in soil:

[0050] Soil pretreatment: Plow the arsenic-contaminated soil, dry it, and crush it to control the particle size to less than 2mm;

[0051] Soil treatment process with microbial preparations: Add 0.2 kg of microbial preparations prepared in Examples 1-2 to 1000 kg of arsenic-contaminated soil samples, stir evenly, and incubate for 7 days to ensure that the soil moisture content is about 20-30%. After 7 days, detect the arsenic content in the exchangeable state, see Table 1 for details;

[0052] Table 1

[0053] group before processing Example 1 group Example 2 group Arsenic concentration (mg / kg) 97.4 4.79 5.01

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Abstract

The invention belongs to the technical field of microorganisms and discloses a microbial preparation for remediation of soil arsenic contamination. A fermented liquid of Bacillus licheniformis, a fermented liquid of Pseudomonas mendocina, a fermentation liquid of Nocardia corallina, a fermentation liquid of Arthrobacter crystallopoietes, a fermentation liquid of pseudomonas aeruginosa and a fermentation liquid of Clostridium papyrosolvens are uniformly mixed according to a certain volume ratio to form a mixed bacterial liquid, and the mixed bacterial liquid and a carrier are mixed according to a mass ratio of 1: 3 to form the microbial preparation. The microbial preparation realizes coordinate coexistence, has a reasonable formula and can effectively repair the arsenic-contaminated soil.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbial preparation for repairing soil arsenic pollution. Background technique [0002] Arsenic pollution refers to environmental pollution caused by arsenic or its compounds. The mining and smelting of arsenic and arsenic-containing metals, the production of glass, pigments, technical materials, and paper using arsenic or arsenic compounds as raw materials, and the burning of coal can all produce arsenic-containing waste water, waste gas and waste residue, which will pollute the environment . In addition to natural causes such as rock weathering and volcanic eruptions, atmospheric arsenic pollution mainly comes from industrial production, the use of arsenic-containing pesticides, and coal combustion. Wastewater containing arsenic, pesticides and smoke will pollute the soil. Arsenic accumulates in the soil and thus enters crop tissue. Arsenic and arseni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/40B09C1/10
CPCB09C1/10C09K17/40
Inventor 不公告发明人
Owner SHENZHEN KUAKE BIOSCI TECH CO LTD
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