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Pseudomonas mendocina YG8, seed liquid and preparation method and application of seed liquid

A technology of Pseudomonas and YG8, applied in the field of microorganisms, can solve the problems of inhibiting the growth of strains and denitrification performance, and achieve high removal rate and great application value

Active Publication Date: 2020-01-07
LONGYAN UNIV
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Problems solved by technology

[0005] The currently discovered heterotrophic nitrifying-aerobic denitrifying bacteria mainly include Bacillus, Alcaligenes, Paracoccus and Pseudomonas. Nitrite nitrogen has certain toxicity to microorganisms, which will inhibit the growth and denitrification performance of strains. Therefore, heterotrophic nitrification-aerobic denitrification can have high removal rates for ammonia nitrogen, nitrate nitrogen and nitrite nitrogen at the same time less strains

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  • Pseudomonas mendocina YG8, seed liquid and preparation method and application of seed liquid
  • Pseudomonas mendocina YG8, seed liquid and preparation method and application of seed liquid
  • Pseudomonas mendocina YG8, seed liquid and preparation method and application of seed liquid

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Embodiment

[0061] A Pseudomonas strain YG8 of the present invention, the Pseudomonas strain YG8 (Pseudomonas mendocinaYG8) is a heterotrophic nitrifying-aerobic denitrifying bacterium, preserved in the Guangdong Provincial Microbial Culture Collection Center, and the preservation time is 2018 On March 21, the deposit number is GDMCC NO: 60340.

[0062] 1) Separation and screening of strains

[0063] The Pseudomonas YG8 of the above-mentioned present embodiment is mainly screened by the following methods:

[0064] 1.1) Primary screening

[0065] The activated sludge collected from the Longjin Sewage Treatment Plant in Longyan City, Fujian Province was added to the Erlenmeyer flask containing the enrichment medium with glass beads for enrichment culture. After the enrichment, the bacterial solution was diluted and spread on the beef extract peptone solid plate. After a single colony grows, it is further streaked and purified. Pick the purified strains and insert them into the sterilize...

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Abstract

The invention discloses pseudomonas mendocina YG8. The pseudomonas mendocina YG8 (pseudomonas mendocina YG8) is a heterotrophic Nitrification-aerobic denitrification strain, and is preserved in Guangdong Province Microbiological Culture Collection Center, the preservation time is 21 March 2018 and the preservation No. is GDMCC NO:60340. The pseudomonas mendocina YG8 has high removal rate on ammonia nitrogen, nitrate nitrogen and nitrite nitrogen, can provide an inoculum source for biological treatment on complex nitrogen-containing biological waste water, and has great application value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Pseudomonas strain YG8, a seed solution and a preparation method and application thereof. Background technique [0002] With the rapid development of social economy and the intensification of urbanization and industrialization, people pay more and more attention to the prevention and control of water pollution. How to solve the increasingly serious water eutrophication phenomenon has become an important aspect of water pollution control. Eutrophication of water body endangers the survival of aquatic organisms, affects the ecological environment, and increases the difficulty and cost of water treatment. Excessive nitrate concentration in drinking water can easily lead to methemoglobinemia. [0003] Biological denitrification technology is widely used due to its economical, efficient, easy to operate, and no secondary pollution. The traditional theory holds that nitrifi...

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38
CPCC12N1/20C02F3/34C02F2101/16C12R2001/38C12N1/205Y02W10/10
Inventor 夏春雨孙巍杨雅玲洪维祎丁燕玲张丽娟黄燕高佳慧李金连王浩陈传志林剑南黄志君
Owner LONGYAN UNIV
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