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31 results about "Porcine tissue" patented technology
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Porcine tissue is the basis for hundreds of medical and cosmetic products currently on the market and in research and development. We supply various tissues including, bones, veins, cornia, eyes, brain material, joints, organs, glands, skin, blood, tendons, fetal, reproductive, and digestive materials,...
Porcine pseudorabies virus strain as well as inactivated vaccine and applications thereof
ActiveCN103305474APromote rapid proliferationHigh titerMicroorganism based processesAntiviralsLaboratory cultureVirus strain
The invention discloses a porcine pseudorabies virus strain as well as an inactivated vaccine and applications thereof, belonging to the field of separation and application of the porcine pseudorabies virus strain. The invention firstly provides a porcine pseudorabies virus BJ strain separated from diseased pig tissues, and the microbial preservation number of the porcine pseudorabies virus BJ strain is CGMCC (China General Microbiological Culture Collection Center) No.7351. The invention discloses a method for preparing the inactivated vaccine by applying the porcine pseudorabies virus BJ strain. The method comprises the steps of culturing a virus strain to obtain a virus solution; adding an inactivator, and inactivating and concentrating the virus solution; and evenly mixing an adjuvant and the virus solution, and emulsifying to obtain the inactivated vaccine. The technological parameters of the inactivated vaccine preparation method are further optimized, and the immune protection efficacy and safety of the inactivated vaccine can be improved. Shown by the immune protection efficacy and safety tests, the porcine pseudorabies inactivated vaccine prepared has good immune protection efficacy and safety, and can be clinically used for preventing or treating porcine pseudorabies.
Owner:泰州博莱得利生物科技有限公司 +2
Method of Separating Collagen From the Various Animal Tissues for Producing Collagen Solution and Product Using the Same
ActiveUS20080118947A1Improve customer satisfactionQuality improvementConnective tissue peptidesPeptide/protein ingredientsPhosphateFractionation
A method for separation the collagen from the various animal tissues is disclosed for preparing collagen solution and product using the same. The porcine tissues are processed to have proper form and size for acid-treatment. The acid-treatment is repeated with pepsin to separate type I or II collagens. The separated collagen is salt-treated for fractionation and ethanol-treated for obtaining 5˜10% of collagen from the initial tissue weight. The prepared tissues are processed for separating collagen through the collagen separating process. The separated collagen is processed for preparing product. The method for preparing product is comprised: treating a collagen solution having a predetermined concentration under a neutral condition at a low temperature, followed by overnight treatment at a temperature of 30 to 35° C.; concentrating collagen by centrifugation; and dissolving the thus-concentrated collagen in refrigerated weakly-acidic solvent or phosphate buffered saline (PBS), thereby preparing collagen having a concentration of 1 to 5 mg / mL.
Owner:CELLONTECH
Method for enzymaticly digesting porcine fat tissue and applications thereof
InactiveCN105018210ANatural dissolutionReduce moisture contentFatty-oils/fats refiningFatty-oils/fats productionTissue proteinEnzymatic digestion
The present invention relates to a method for enzymaticly digesting porcine fat, wherein fat cells are fragmented by using an ultrasonic wave-assisted enzymatic digestion method. The present invention also provides a method that is based on the foregoing method and that is for further refinement of advanced edible lard and fat tissue protein hydrolysate. The method comprises: extracting lard in in a cryogenic condition; obtaining the advanced edible lard after refining the lard; and obtaining a sauce by performing homogenizing, flavouring, thermal reacting, filling, and sterilizing on the protein hydrolysate obtained during enzymatic extraction.
Owner:CHINA MEAT RES CENT
PCR primer pair for identifying or assisting identification of animal tissue / or organ and application thereof
InactiveCN102312003AHigh precisionStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationAgricultural scienceDNA
The invention discloses a PCR primer pair for identifying or assisting identification of an animal tissue / or an organ and application thereof. The primer pair provided by the invention is at least one from five PCR primer pairs for identifying or assisting identification of cattle, sheep, pig, chicken and duck tissue / or organ, wherein the PCR primer pair for identifying or assisting identification of pig tissue / or organ is always included. The five PCR primer pairs for identifying or assisting identification of cattle, sheep, pig, chicken and duck tissue / or organ respectively consist of two DNA single chains shown as a sequence 1 and a sequence 2, two DNA single chains shown as a sequence 3 and a sequence 4, two DNA single chains shown as a sequence 5 and a sequence 6, two DNA single chains shown as a sequence 7 and a sequence 8, and two DNA single chains shown as a sequence 9 and a sequence 10 in the sequence table. An annealing temperature of 62 DEG C is employed for the five primer pairs to complete all PCR identifications at once and at a same temperature; besides, the PCR primer pair has strong singularity and a short detection time.
Owner:CHINA ANIMAL DISEASE CONTROL CENT
Method of separating collagen from the various animal tissues for producing collagen solution and product using the same
ActiveUS7781158B2Efficient separationImprove quality and reliabilityConnective tissue peptidesPeptide/protein ingredientsPhosphateFractionation
A method for separation the collagen from the various animal tissues is disclosed for preparing collagen solution and product using the same. The porcine tissues are processed to have proper form and size for acid-treatment. The acid-treatment is repeated with pepsin to separate type I or II collagens. The separated collagen is salt-treated for fractionation and ethanol-treated for obtaining 5˜10% of collagen from the initial tissue weight. The prepared tissues are processed for separating collagen through the collagen separating process. The separated collagen is processed for preparing product. The method for preparing product is comprised: treating a collagen solution having a predetermined concentration under a neutral condition at a low temperature, followed by overnight treatment at a temperature of 30 to 35° C.; concentrating collagen by centrifugation; and dissolving the thus-concentrated collagen in refrigerated weakly-acidic solvent or phosphate buffered saline (PBS), thereby preparing collagen having a concentration of 1 to 5 mg / mL.
Owner:CELLONTECH
Seneca valley virus SVV-ZM-201801 and application thereof
ActiveCN109554352APromote rapid proliferationHigh titerSsRNA viruses positive-senseViral antigen ingredientsCytopathic effectCulture cell
The invention provides a Seneca valley virus SVV-ZM-201801 and an application thereof. Separation is performed from pig tissues, and sub-culture and plaque purification are performed, so that the Seneca valley virus is obtained. The separated strain can be stably proliferated on sub-culture cells, typical cytopathic effects are formed, and the virus titer is as high as 108.5-1010.5TCID50 / mL. The separated strain does not have obvious pathogenicity on piggies. The Seneca valley virus separated strain has good proliferating properties, and is good in immunogenicity. Vaccines prepared from the separated strain can induce the piggies to generate high-level neutralizing antibodies, and powerful technical support is provided for effective prevention and control of the Seneca valley virus.
Owner:CHINA ANIMAL HUSBANDRY IND
A Confirmatory Analytical Method for the Detection of Piperazine Residues in Poultry and Pig Tissues
The invention relates to the field of detection of veterinary drug residues, in particular to a confirmatory analysis method for efficiently detecting piperazine residues in poultry and pig tissues (muscle, liver, kidney and sebum). In this method, tissue samples are extracted, purified and derivatized by accelerated solvent extraction, and then detected by gas chromatography-tandem mass spectrometry (GC / MS-MS). Compared with other detection methods in terms of recovery rate, precision and sensitivity, the present invention has the advantages of simplicity, speed, accuracy and sensitivity.
Owner:YANGZHOU UNIV
Elimination of endogenous porcine retrovirus
Porcine nucleic acid sequences flanking potentially infectious porcine endogenous retroviral (PERV) insertion sites have been identified and isolated. The unique flanking sequences include porcine nucleic acid sequences that flank the 3′ end and porcine nucleic acid sequences that flank the 5′ end of PERV insertion sites. The present invention provides compositions and methods for detecting presence of PERV in a sample, particularly those with infectious potential. In addition, the invention relates to breeding of pigs or selection of porcine tissue that is free of infectious PERV for use as a xenotransplant tissue.
Owner:MAYO FOUND FOR MEDICAL EDUCATION & RES
Medicinal kallikrein and preparing method and application thereof
ActiveCN107058269AHigh purityImprove biological activityHydrolasesPeptide/protein ingredientsSide effectKinin
The invention relates to medicinal kallikrein (KLK1) and a preparing method and application thereof. The kallikrein does not contain KLK1c. Tissue kallikrein from porcine pancreas is composed of three components with different glycosylation degrees. A protein electrophoresis analysis result is three stripes, named KLK1b, KLK1a and KLK1c respectively in a molecular weight descending order, wherein KLK1c has the lowest glycosylation degree and the lowest specific activity. Compared with existing tissue kallikrein from porcine pancreas available in the market, the tissue kallikrein with KLK1c removed has the advantages of being higher in purity and stability, higher in biological activity, good in pharmacodynamic effect and smaller in side effect. The invention also provides a method for purifying porcine tissue kallikrein. The problem that KLK1b, KLK1a and KLK1c are hard to separate and industrialization can not be achieved is solved.
Owner:ZONHON BIOPHARMA INST +1
Nucleotide sequence for detecting swine NLRP6 (nod-like receptor family pyrin domain-containing protein 6) and application of nucleotide sequence
InactiveCN105886655AQuick checkAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationFluorescenceNucleotide
The invention discloses a nucleotide sequence for detecting swine NLRP6 (nod-like receptor family pyrin domain-containing protein 6) and application of the nucleotide sequence. The nucleotide sequence for detecting the swine NLRP6 is shown as sequences SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 in a sequence table. The sequences SEQ ID NO.1 and the SEQ ID NO.2 respectively represent sense primers and antisense primers for detecting the swine NLRP6, and the sequences SEQ ID NO.3 represent fluorescent probes for detecting the swine NLRP6. The invention further discloses a method for detecting the swine NLRP6 by the aid of the primers and the probes. The nucleotide sequence, the application and the method have the advantages that as shown by results, the method is good in specificity and high in sensitivity, expression of the swine NLRP6 in swine tissue and organ samples can be quickly and accurately detected, the swine NLRP6 can be monitored in real time when NLRP6 inflammasome is researched, inflammation occurrence procedures and development procedures can be timely understood and mastered, and the nucleotide sequence, the application and the method have important theoretical significance and practical significance.
Owner:TIANJIN INST OF ANIMAL HUSBANDRY & VETERINARY
Antigenic fusion protein carrying Galalpha 1,3Gal epitopes
InactiveUS20050255099A1Easy and cheap to produceSimple designOrganic active ingredientsPeptide/protein ingredientsEpitopeHeterografts
Owner:RECOPHARMA AB
Porcine neuronal interleukin B polypeptide, and preparation method and application of polyclonal antibody thereof
The invention belongs to the field of biochemistry and molecular immunology, and relates to a porcine neuronal interleukin B polypeptide, and a preparation method and application of a polyclonal antibody thereof. The preparation method comprises the following steps: coupling C-terminal modified polypeptide of the porcine neuronal interleukin B polypeptide with a carrier protein keyhole limpet hemocyanin KLH to obtain an NMB modified polypeptide-KLH coupled protein; emulsifying the NMB modified polypeptide-KLH coupling protein, and immunizing New Zealand white rabbits; collecting and separatingto obtain serum containing a rabbit anti-pig NMB modified polypeptide antibody; and purifying the prepared NMB polyclonal antibody by adopting a Protein G purification column method. A polypeptide antigen and the polyclonal antibody have the characteristics of simple preparation method, low cost and high titer, and can specifically bind to an NMB protein in pig tissues.
Owner:YANGZHOU UNIV
Rapid test card for simultaneously detecting PEDV and TGEV and preparation and use methods thereof
ActiveCN111596064AEasy to operateImprove anti-interference abilityBiological testingImmunoassaysAntiendomysial antibodiesMedicine
The invention discloses a rapid test card for simultaneously detecting PEDV and TGEV, and preparation and application methods thereof. The rapid test card comprises a card shell and a test strip arranged in the card shell. The test strip comprises a PVC bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad, the sample pad, wherein the combination pad, the nitrocellulose membrane and the absorption pad are sequentially connected and pasted to the PVC bottom plate; and a sample adding hole and a result observation window are formed in the card shell. An anti-PEDV specific monoclonal antibody and an anti-TGEV specific monoclonal antibody are fixed on the nitrocellulose membrane and are used for respectively forming a first detection line and a second detection line; and the anti-PEDV specific monoclonal antibody marked by colloidal gold and the anti-TGEV specific monoclonal antibody marked by colloidal gold are fixed on the combination pad. According to the present invention, with the rapid detection card, the PEDV and the TGEV in a pig tissue excrement sample can be simultaneously detected, the sample adding only needs one time, the reaction is performed under the unified condition, and the rapid detection card has advantages of rapidness, high accuracy, high sensitivity, simple operation and the like.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
African swine fever virus rapid detection test strip and preparation method and application thereof
ActiveCN111474357AThe detection process is fastStrong specificityBiological testingImmunoassaysAfrican swine feverAntibodies monoclonal
The invention provides an African swine fever rapid detection test strip. The African swine fever rapid detection test strip is composed of a test strip and a sample treatment liquid. On the other hand, the invention also provides a method for preparing the test strip. The method comprises the following steps of 1) preparing an anti-African swine fever virus P30 protein monoclonal antibody and ananti-African swine fever virus P72 protein monoclonal antibody, 2) preparing a latex microsphere pad and a sample pad, 3) preparing a nitrocellulose membrane detection line and a quality control line,4) preparing and sub-packaging a sample treatment solution, and 5) assembling test strips. The African swine fever rapid detection test strip provided by the invention is suitable for detection of African swine fever viruses in porcine serum, porcine whole blood and porcine tissues, is strong in specificity, high in sensitivity, good in stability and high in detection speed, can be used for earlyscreening of the African swine fever virus, and is particularly suitable for on-site African swine fever infection diagnosis, epidemiological investigation, pig international trade quarantine inspection and the like.
Owner:杭州恒奥科技有限公司 +1
Porcine-derived osteopontin (OPN) monoclonal antibody, hybridoma cell strain thereof, and applications of monoclonal antibody and hybridoma cell strain
ActiveCN111172116AImprove reproductive performanceImmunoglobulins against cytokines/lymphokines/interferonsBiological material analysisBiochemistryCell strain
The invention discloses a porcine-derived osteopontin (OPN) monoclonal antibody, a hybridoma cell strain thereof, and applications of the monoclonal antibody and the hybridoma cell strain. The hybridoma cell strain secreting the porcine-derived OPN monoclonal antibody is classified and named as a hybridoma cell strain Cy2, and is preserved in China Center for Type Culture Collection (CCTCC) on Dec.19,2019, wherein the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO:C2019293.The porcine-derived OPN monoclonal antibody is secreted by the hybridomacell strain or a passage cell strain of the hybridoma cell strain. The monoclonal antibody is suitable for rapidly and accurately detecting the content of OPN protein in pig tissues, semen or pig oocytes, so that the monoclonal antibody can be used for preparing related detection kits or colloidal gold test paper, and a firm foundation is laid for deep research of porcine-derived OPN.
Owner:SOUTH CHINA AGRI UNIV
Elimination of endogenous porcine retrovirus
InactiveUS20030224350A1High degree of sensitivityStrong specificityFungiSugar derivativesNucleic acid sequencingEndogenous retrovirus
Porcine nucleic acid sequences flanking potentially infectious porcine endogenous retroviral (PERV) insertion sites have been identified and isolated. The unique flanking sequences include porcine nucleic acid sequences that flank the 3' end and porcine nucleic acid sequences that flank the 5' end of PERV insertion sites. The present invention provides compositions and methods for detecting presence of PERV in a sample, particularly those with infectious potential. In addition, the invention relates to breeding of pigs or selection of porcine tissue that is free of infectious PERV for use as a xenotransplant tissue.
Owner:MAYO FOUND FOR MEDICAL EDUCATION & RES
Method for detecting pig fat deposition by using phylaxin expression level
The invention relates to a method for detecting pig fat deposition by using phylaxin expression level, which comprises the following steps: 1) extracting total DNA from detected pig tissue, carrying out inverse transcription to obtain a cDNA sample; 2) carrying out PCR amplification on the cDNA sample from the step 1) by primer pair shown in SEQ ID NO:2 and 3 to obtain a gene fragment shown in SEQ ID NO:1; 3) carrying out PCR amplification on the housekeeping gene by primer pair shown in SEQ ID NO:5 and 6 to obtain a gene fragment shown in SEQ ID NO:4; 4) carrying out agarose gel electrophoresis on the PCR products from the step 2) and step 3), comparing the optical density value in a gel imaging system. According to the invention, the fat pigs and lean type pigs with 1 month age, 3 months age, 5 months age and 7 months age can be taken as detection objects, the expression level of fat cytokine phylaxin (resistin) can be detected, the protein level of resistin is taken for reflecting the deposition condition of the pig fat. The method of the invention has the advantages of rapidity, convenience, accuracy and no influence on normal growth of pigs, and is suitable for dynamically monitoring the fat deposition condition of pigs in the culture process.
Owner:HUAZHONG AGRI UNIV
Triple fluorescent quantitative PCR primer group for detecting different types of porcine circovirus, kit and application
PendingCN113403430AIncreased sensitivitySpecific strongMicrobiological testing/measurementDNA/RNA fragmentationCircovirusDiagnosis laboratory
The invention provides a triple fluorescent quantitative PCR primer group for detecting different types of porcine circovirus, a kit and application, and relates to the technical field of biological detection. According to the triple fluorescent quantitative PCR primer group for detecting different types of porcine circovirus, primers and probes are synthesized according to conserved sequences of porcine circovirus type 2, porcine circovirus type 3 and porcine circovirus type 4 in Genbank, and meanwhile, the kit is further constructed and used for porcine epidemic disease detection. 83 porcine tissue samples from Zhejiang Province are detected by utilizing an established fluorescent quantitative PCR system, and a result shows that the primer group is good in specificity, repeatability and stability, relatively high in consistency and relatively high in accuracy. According to the triple fluorescent quantitative PCR primer group for detecting different types of porcine circovirus, the lowest detection values of PCV2 and PCV3 are both 10 copies / microliter, the lowest detection value of PCV4 is 1 copy / microliter, and the defects of low sensitivity, prone laboratory pollution and the like of the current triple PCR detection method for the porcine circovirus type 2, the porcine circovirus type 3 and the porcine circovirus type 4 are overcome.
Owner:浙江省动物疫病预防控制中心
PCR primer pair for identifying or assisting identification of animal tissue / or organ and application thereof
InactiveCN102312003BHigh precisionStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyZoology
The invention discloses a PCR primer pair for identifying or assisting identification of an animal tissue / or an organ and application thereof. The primer pair provided by the invention is at least one from five PCR primer pairs for identifying or assisting identification of cattle, sheep, pig, chicken and duck tissue / or organ, wherein the PCR primer pair for identifying or assisting identification of pig tissue / or organ is always included. The five PCR primer pairs for identifying or assisting identification of cattle, sheep, pig, chicken and duck tissue / or organ respectively consist of two DNA single chains shown as a sequence 1 and a sequence 2, two DNA single chains shown as a sequence 3 and a sequence 4, two DNA single chains shown as a sequence 5 and a sequence 6, two DNA single chains shown as a sequence 7 and a sequence 8, and two DNA single chains shown as a sequence 9 and a sequence 10 in the sequence table. An annealing temperature of 62 DEG C is employed for the five primer pairs to complete all PCR identifications at once and at a same temperature; besides, the PCR primer pair has strong singularity and a short detection time.
Owner:CHINA ANIMAL DISEASE CONTROL CENT
A kind of porcine igfn1 protein polyclonal antibody and its preparation method and application
ActiveCN104910268BHigh detection specificityAntigen sequence lengthSerum immunoglobulinsImmunoglobulins against animals/humansNucleotideEngineered genetic
The invention discloses a pig IGFN1 protein polyclonal antibody, further discloses antigenic polypeptide for inducing the pig IGFN1 protein polyclonal antibody and belongs to the field of animal gene engineering. The antigenic polypeptide is formed by the amino acid sequence as shown by SEQ ID No: 1. The nucleotide sequence shown by SEQ ID No: 2 forms the antigenic polypeptide encoding genes. The pig IGFN1 protein polyclonal antibody is high in pig IGFN1 protein expression level detection specificity, and an important tool is provided for expression analysis and correlational studies of IGFN1 in pig tissues. The antigen sequence is long, and the detection sensitivity is high.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Pretreatment kit for detecting ochratoxin a in porcine tissue and its application
InactiveCN104391057BReduce false positivesImprove accuracyComponent separationMass Spectrometry-Mass SpectrometryElution
The invention provides a pretreatment kit for detecting ochratoxin A in pig tissue. The pretreatment kit is characterized by comprising a buffer solution, an extracting solution 1, an extracting solution 2, an IAC column, a leacheate and an elution solution. The invention also provides a method for detecting the ochratoxin A in the pig tissue by utilizing the pretreatment kit. The method is characterized in that after the pig tissue is pretreated by utilizing the kit, the ochratoxin A is quantitatively detected by utilizing liquid chromatography-mass spectrometry under a certain liquid and mass spectrometry condition. The kit and the method have characteristics of low detection limit and high sensitivity and are relatively high in stability, precision and accuracy.
Owner:重庆市动物疫病预防控制中心
Method for detecting menbutone residues in porcine tissues
The invention discloses a method for detecting a menbutone residue in pig tissue. The method comprises the steps as follows: firstly, the pig tissue subjected to intramuscular menbutone injection is weighed, cut up and then subjected to homogenate, and the tissue subjected to homogenate is extracted by using acetonitrile, then after centrifugation, the extracted liquid is subjected to rotary evaporation in vacuum with the temperature of 60 DEG C to dry, and after cooling, moving phase is added into the residue for ultraphonic dissolution and then centrifugation, and a liquid supernatant is collected and filtered by a millipore filter membrane with filtering particle diameter of 0.22 mu m, and the filter liquor is used as a test solution; secondly, a menbutone reference substance is weighed precisely, and after dissolved by acetonitrile and diluted by moving phase, the reference substance has a concentration of 9 to 11 mu g / mL, and a reference substance solution is obtained; thirdly, HPLC (High-Performance Liquid Chromatography) is adopted for testing the test solution and the reference substance solution, the chromatogram is recorded, and the menbutone residue in the tissue is calculated by peak area according to external standard method. The method is good in specificity, high in sensitivity and good in repeatability, can quickly detect the menbutone residue in the pig tissue, and is a reliable method for controlling the quantity of the menbutone residue in animal-origin food.
Owner:SOUTHWEST UNIV
Freeze-dried microchip, kit and method for detecting African swine fever virus
PendingCN111455095ASensitive detectionImprove accuracyMicrobiological testing/measurementDNA/RNA fragmentationAfrican swine feverPorcine blood
The invention discloses a freeze-dried microchip, a kit and a method for detecting African swine fever virus in pig tissue and pig blood feed products. The freeze-dried microchip is characterized in that a fluorescent PCR reaction system for detecting the African swine fever virus is fixed on the microchip through freeze-drying; the kit comprises a freeze-dried microchip, a bottle of mineral oil,a tube of positive control, a tube of negative control, a tube of diluent and a tube of nuclease-free water. A primer probe group is used for identifying the African swine fever (ASF) virus, and detecting whether to-be-detected pig tissue and a pig blood feed product sample contain the African swine fever virus or not. By utilizing the freeze-dried microchip, the kit and the method provided by theinvention, ASF virus is detected from pig tissue and pig-derived feed for the first time, it is proved that the freeze-dried microchip has an important application value on prevention and control ofthe ASF, is beneficial to finding and cutting off a virus transmission chain, and can effectively prevent large-scale outbreak.
Owner:CHINA ANIMAL DISEASE CONTROL CENT
Porcine opn monoclonal antibody, its hybridoma cell line and application
ActiveCN111172116BImprove reproductive performanceImmunoglobulins against cytokines/lymphokines/interferonsBiological material analysisCell strainHybridoma cell
The invention discloses a porcine OPN monoclonal antibody, its hybridoma cell line and application. The hybridoma cell line secreting porcine OPN monoclonal antibody, which is classified as hybridoma cell line Cy2, was deposited in the China Center for Type Culture Collection (CCTCC) on December 19, 2019, and the preservation address is Wuhan, China .Wuhan University, the preservation number is CCTCC NO: C2019293. The porcine OPN monoclonal antibody is secreted and produced by the above-mentioned hybridoma cell line or its passaged cell line. The monoclonal antibody is suitable for quickly and accurately detecting the content of OPN protein in porcine tissue, semen or porcine oocytes. Therefore, the monoclonal antibody can be used to prepare related detection kits or colloidal gold test paper, etc. The in-depth research of OPN has laid a firm foundation.
Owner:SOUTH CHINA AGRI UNIV
Preparation method of multiple decellularized material of porcine small intestinal submucosa modified by nucleus pulposus cells
ActiveCN107715177BStrong adhesion growth abilityImprove practicalityPharmaceutical delivery mechanismTissue regenerationOrganosolvSubmucosa
Owner:ZHEJIANG UNIV
Medicinal kininogenase and its preparation method and application
ActiveCN107058269BEfficient use ofHigh purityPeptide/protein ingredientsHydrolasesKininDrug biological activity
The invention relates to medicinal kallikrein (KLK1) and a preparing method and application thereof. The kallikrein does not contain KLK1c. Tissue kallikrein from porcine pancreas is composed of three components with different glycosylation degrees. A protein electrophoresis analysis result is three stripes, named KLK1b, KLK1a and KLK1c respectively in a molecular weight descending order, wherein KLK1c has the lowest glycosylation degree and the lowest specific activity. Compared with existing tissue kallikrein from porcine pancreas available in the market, the tissue kallikrein with KLK1c removed has the advantages of being higher in purity and stability, higher in biological activity, good in pharmacodynamic effect and smaller in side effect. The invention also provides a method for purifying porcine tissue kallikrein. The problem that KLK1b, KLK1a and KLK1c are hard to separate and industrialization can not be achieved is solved.
Owner:ZONHON BIOPHARMA INST +1
Sterile autologous, allogenic or xenogenic implant and the method of its production
The subject of the invention is a sterile, dehydrated acellular implant, which during its rehydration by water or bodily fluids displays anisotropic expansion and can act as a substrate for adhesion, migration and growth of live cells. Collagen structures of the transplant are at least partially denatured through the action of heat or organic solvents, such as lower aliphatic alcohols and ketones, which simultaneously act as preservative and sterilization agents, especially for certain types of viruses. The implant is sterilized by radiation while in an substantially dehydrated state, preferably by accelerated electrons. The transplant can be derived from various animal tissues, especially mammalian tissues, such as human or porcine tissues. The tissues suitable for the invention can be, for example, skin, placenta, pericardium, peritoneum, intestinal wall, tendon, blood vessel, etc. The implant is suitable for use in human and veterinary medicine, for instance as a temporary wound and burn cover, for the repair, substitution and regeneration of tissues, and also as a substrate for laboratory cell cultivation.
Owner:MEDICEM TECH SRO
Seneca Valley Virus svv-zm-201801 and Its Applications
ActiveCN109554352BPromote rapid proliferationHigh titerSsRNA viruses positive-senseViral antigen ingredientsCytopathic effectCulture cell
The invention provides a Seneca valley virus SVV-ZM-201801 and an application thereof. Separation is performed from pig tissues, and sub-culture and plaque purification are performed, so that the Seneca valley virus is obtained. The separated strain can be stably proliferated on sub-culture cells, typical cytopathic effects are formed, and the virus titer is as high as 108.5-1010.5TCID50 / mL. The separated strain does not have obvious pathogenicity on piggies. The Seneca valley virus separated strain has good proliferating properties, and is good in immunogenicity. Vaccines prepared from the separated strain can induce the piggies to generate high-level neutralizing antibodies, and powerful technical support is provided for effective prevention and control of the Seneca valley virus.
Owner:CHINA ANIMAL HUSBANDRY IND
A kind of porcine neurointermediate b polypeptide and its polyclonal antibody preparation method and application
The invention belongs to the field of biochemistry and molecular immunology, and relates to a preparation method and application of a porcine neurointermediate B polypeptide and its polyclonal antibody. Blue protein KLH coupling to obtain NMB modified polypeptide-KLH coupled protein; emulsify NMB modified polypeptide-KLH coupled protein and immunize New Zealand white rabbits; collect and separate serum containing rabbit anti-pig NMB modified polypeptide antibody; use Protein G to purify The prepared NMB polyclonal antibody was purified by column method. The polypeptide antigen and polyclonal antibody have the characteristics of simple preparation method, low cost and high titer, and can specifically bind NMB protein in pig tissue.
Owner:YANGZHOU UNIV
Detection method and primers for evaluating enrichment result of porcine histone modification
ActiveCN112011625AEffective enrichment effectEffective determination of enrichment effectMicrobiological testing/measurementMaterial analysisBiotechnologyGenomics
The invention provides a detection method and primers for evaluating an enrichment result of porcine histone modification. The method comprises the following steps: enriching DNA combined with targetprotein by using a pig tissue sample through a ChIP-seq method, carrying out quantitative detection by adopting a fluorescent quantitative QPCR method, and judging the result, wherein a calculation method of the quantitative detection result adopts a delta delta Ct method for judgment, and the result is judged as follows: if the effective precipitation enrichment multiple of positive primers and negative primers is greater than or equal to 2, the enrichment effect is considered to reach the standard, and if the effective precipitation enrichment multiple of positive primers and negative primers is less than 2, the enrichment effect is considered to not reach the standard. According to the method, the v histone modification level can be specifically detected, and the DNA region enrichment effect can be effectively judged, so that important reference is provided for ChIP-seq, the time and economic cost caused by poor sequencing result due to poor enrichment effect are greatly saved, andan effective way is provided for developing pig epigenomics research.
Owner:WUHAN IGENEBOOK BIOTECH CO LTD
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