30 results about "Pestivirus infections" patented technology

The disclosed invention is a bicyclo[4.2.1]nonane and its pharmaceutically acceptable salt or prodrug, and its composition and method of use to treat Flaviviridae (Hepacivirus, Flavivirus, and Pestivirus) infections in a host, including animals, and especially humans.

The invention relates to vaccines used in the eradication or control of pestivirus infections, particularly those used in pigs or ruminants. The invention provides nucleic acid, pestivirus-like particles and a pestivirus vaccine, comprising the nucleic acid or particles, which is capable of eliciting a proper immune response without having the ability to spread throughout the vaccinated animal, thereby avoiding the negative consequences of viral spread. Preferably, the immune response allows for serological discrimination between vaccinated animals and wild-type pestivirus infected animals.

The present invention relates to clone of IRG6 gene having a potential anti-classical swine fever virus effect, and construction of a stable expression cell line of the IRG6 gene. The construction is characterized by comprising the following steps: (1) obtaining a gene fragment; (2) constructing a vector; (3) screening a stable IRG6 protein expression cell line; and (4) detecting reporter gene expression. The cell line can be used for classical swine fever virus infection mechanism research, wherein drugs for treating classical swine fever virus can be developed by revealing the anti-classical swine fever virus mechanism of the protein gene.

Porcine pestivirus designated herein as atypical porcine pestivirus (“APPV”) (Genbank accession no. KR011347.1). Immunogenic compositions to induce an immune response against porcine pestivirus infection in a pig are described, which APPV antigenic agents (e.g., isolated whole virus, derivatives thereof, functional fragments thereof, and combinations of the foregoing). Methods of vaccinating against porcine pestivirus infection using the immunogenic compositions are also described. The methods can be also applied for clinical research and / or study, including diagnostic methods for detecting pestivirus infection using monoclonal antibodies specifically binding to APPV epitopes.

The present invention mainly relates to an affinity peptide that can be combined with classical swine fever virus E2 protein and its application. The sequence of the affinity peptide is HRKWKSKWK(P 7 ). The present invention uses the crystal structure of the E2 protein of bovine viral diarrhea virus as a template, and obtains the three-dimensional structure of the E2 protein of classical swine fever virus through homology modeling, and finally obtains a polypeptide sequence with a high score value, which is P 7 ; Synthetic P 7 ELISA and SPR tests were used to identify the affinity and specificity of its interaction with E2 protein, and the results showed that P 7 It has a high affinity and specific binding to E2; then its activity of inhibiting virus infection is verified by qRT‑PCR and IPMA tests, and the results show that P 7 The sequence has better activity of inhibiting the infection of PK‑15 cells by classical swine fever virus. The invention can provide a reliable theoretical basis and new ideas for further research on viral receptors and antiviral drug design.

The invention relates to a colloidal gold detection card for rapidly detecting rabbit plague virus and a preparation method thereof. The specific steps are: (1) preparation of paired anti-rabbit plague virus monoclonal antibodies, including immune procedures, cell fusion, hybridoma screening and Cloning, antibody preparation and purification; (2) colloidal gold detection card for rapid detection of rabbit plague virus and its preparation method, including preparation of colloidal gold monoclonal antibody, sample pad treatment, spray film, C and T line determination, tested Sample processing, performance measurement. The invention can quickly, sensitively and accurately detect rabbit plague virus infection in the liver and kidney of rabbits, overcomes the domestic method of detecting rabbit plague virus mainly by red blood cell agglutination test, greatly shortens the detection time, and provides convenience for on-site detection.

The invention relates to a recombinant E2 protein by using silkworm as a platform to produce a classical swine fever virus gene subgroup 2.1. The invention also provides a subunit vaccine comprising the recombinant protein, which can be used for enhancing the capability of swine to resist classical swine fever virus infection.

The invention discloses a duck Tembusu virus E protein truncated gene, a recombinant duck plague virus, a construction method and an application. The nucleotide sequence of the duck Tembusu virus E protein truncated gene is shown in SEQ ID No.4. A recombinant duck plague virus, wherein the duck Tembusu virus E protein truncated gene is inserted into the genome of the recombinant duck plague virus. The present invention screens and obtains a truncated gene of duck Tembusu virus E protein, inserts the E protein truncated gene into the genome of recombinant duck plague virus to obtain a new recombinant duck plague virus, and infects CEFs After cells, the E protein truncated gene is highly expressed. The recombinant duck plague virus inserted with the E protein truncated gene can be used for the preparation of duck plague virus and duck Tembusu virus dual vaccine.

A polypeptide of the present invention that inhibits the infection activity of classical swine fever virus and its application, the polypeptide sequence is KRWWSHK(P 8 ). The present invention uses the crystal structure of the E2 protein of bovine viral diarrhea virus as a template, and obtains the three-dimensional structure of the E2 protein of classical swine fever virus through homology modeling, and finally obtains a polypeptide sequence with a high score value, which is P 8 ; Synthetic P 8 ELISA and SPR tests were used to identify the affinity and specificity of its interaction with E2 protein, and the results showed that P 8 It has a high affinity and specific binding to E2; then its activity of inhibiting virus infection is verified by qRT‑PCR and IPMA tests, and the results show that P 8 The sequence has better activity of inhibiting the infection of PK‑15 cells by classical swine fever virus. The invention can provide a reliable theoretical basis and new ideas for further research on viral receptors and antiviral drug design.

The invention discloses a method for constructing virus live vector recombinant vaccine by utilizing transposon. Green fluorescent protein is taken as a report gene, expression boxes respectively expressing rabies virus glycoprotein and swine fever E2 protein genes are constructed and are cloned to the shuttle vector of the transposon, under the action of mediation of transposase, recombination with purified canine adenovirus type II virus and herpes virus type I entire genome are respectively carried out, then transfection agent (liposome and the like) is utilized to respectively transfect the recombination product with MDCK and Vero cells, thus obtaining four strains of recombinant viruses taking green fluorescent protein as report gene, namely recombinant canine adenovirus type II virus expressing glycoprotein, recombinant canine adenovirus type II virus expressing E2 protein, recombinant herpes virus type I expressing glycoprotein and recombinant herpes virus type I expressing E2 protein. Immunity test shows that the canine adenovirus type II virus expressing E2 gene and herpes virus type I live vector recombinant vaccine all can induce immunoreaction resistant to swine fever virus infection in swine and canine adenovirus type II virus expressing glycoprotein gene and herpes virus type I live vector recombinant vaccine all can induce immunoreaction resistant to rabies virus infection in dog.

The invention discloses an ELISA kit for detecting a porcine atypical pestivirus antibody based on an Npro protein. The kit comprises a reaction plate coated with porcine atypical pestiviruses havingsequences shown in the formula of SEQ ID NO. 1, an enzyme-labeled antibody, a developing solution, a stop buffer, positive serum and negative serum. The invention creates the ELISA kit for detecting aporcine atypical pestivirus antibody. The kit can fast, specifically and sensitively detect the porcine atypical pestivirus antibody and has antibody detection sensitivity of 1: 1000. The kit is easyto use, realizes a low cost, reaction result observation easiness and good specificity, is suitable for the monitoring of the atypical pestivirus infection, epidemiological investigation and clinicalveterinary sample detection and can be widely used.