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32 results about "Metal affinity chromatography" patented technology
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Metal chelate affinity chromatography is the logical extension technique. Same molecular weight protein can be separated by this technique by using the metal ion containing matrix by chelation, because of their difference in their metal binding ability with proteins.
Nucleic acid separation using immobilized metal affinity chromatography
An immobilized metal affinity chromatography (IMAC) method for separating and / or purifying compounds containing a non-shielded purine or pyrimidine moiety or group such as nucleic acid, presumably through interaction with the abundant aromatic nitrogen atoms in the purine or pyrimidine moiety. The method can also be used to purify compounds containing purine or pyrimidine moieties where the purine and pyrimidine moieties are shielded from interaction with the column matrix from compounds containing a non-shielded purine or pyrimidine moiety or group. Thus, double-stranded plasmid and genomic DNA, which has no low binding affinity can be easily separated from RNA and / or oligonucleotides which bind strongly to metal-charged chelating matrices. IMAC columns clarify plasmid DNA from bacterial alkaline lysates, purify a ribozyme, and remove primers and other contaminants from PCR reactions. The metal ion affinity of yeast RNA decreases in the order: copper (II), nickel (II), zinc (II), and cobalt (II).
Owner:UNIV HOUSTON SYST
Removal of bacterial endotoxin in a protein solution by immobilized metal affinity chromatography
InactiveUS6942802B2Ion-exchange process apparatusIon-exchanger regenerationProtein solutionBacteroides
The present invention relates to the purification of polypeptides and the removal of endotoxin via immobilized metal affinity chromatography (IMAC). More specifically, the invention relates to methods for removing bacterial endotoxin in a protein solution. In specific embodiments, the invention relates to the elimination of endotoxin from Moraxella catarrhalis outer membrane proteins.
Owner:WYETH HOLDINGS CORP
Purification of her-2 variants
The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein which for which the purification process has been especially adapted, as well as means for the preparation of the variant.
Owner:BAVARIAN NORDIC AS
Fixed metal affinity chromatographic stationary phase based on strong chelating ligand and preparation method of stationary phase
InactiveCN103285814AImprove bindingReduce churnHydrolasesOther chemical processesChromatographic separationStationary phase
The invention discloses a fixed metal affinity chromatographic stationary phase which is shown by the structural formula (I) and has strong chelating capability, and a preparation method of the stationary phase. In the structural formula (I), R is methyl or ethyl. The fixed metal affinity chromatographic stationary phase can be obtained by modifying imino-2 (methylene tetrazolium) on the surface of silica gel. The chromatographic stationary phase is used for affinity chromatographic separation of proteins, and has the advantages of good protein separation effect, less metal ion loss and high protein mass recovery rate. The fixed metal affinity chromatographic stationary phase can be applied to rapid separation and purification of genetic engineering products and plasma proteins.
Owner:NORTHWEST UNIV
Removal of bacterial endotoxin in a protein solution by immobilized metal affinity chromatography
InactiveUS20050186218A1Reduce concentrationProtein recovery is greatIon-exchange process apparatusBacterial antigen ingredientsProtein solutionEndotoxin removal
The present invention relates to the purification of polypeptides and the removal of endotoxin via immobilized metal affinity chromatography (IMAC). More specifically, the invention relates to methods for removing bacterial endotoxin in a protein solution. In specific embodiments, the invention relates to the elimination of endotoxin from Moraxella catarrhalis outer membrane proteins.
Owner:WYETH HOLDINGS CORP
Purification of HER-2 variants
The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange chromatography (AIE). The method also provides for an immunogenic variant of HER-2 protein for which the purification process has been especially adapted, as well as means for the preparation of the variant.
Owner:BAVARIAN NORDIC AS
Nucleic acid separation using immobilized metal affinity chromatography
An immobilized metal affinity chromatography (IMAC) method for separating and / or purifying compounds containing a non-shielded purine or pyrimidine moiety or group such as nucleic acid, presumably through interaction with the abundant aromatic nitrogen atoms in the purine or pyrimidine moiety. The method can also be used to purify compounds containing purine or pyrimidine moieties where the purine and pyrimidine moieties are shielded from interaction with the column matrix from compounds containing a non-shielded purine or pyrimidine moiety or group. Thus, double-stranded plasmid and genomic DNA, which has no low binding affinity can be easily separated from RNA and / or oligonucleotides which bind strongly to metal-charged chelating matrices. IMAC columns clarify plasmid DNA from bacterial alkaline lysates, purify a ribozyme, and remove primers and other contaminants from PCR reactions. The metal ion affinity of yeast RNA decreases in the order: copper (II), nickel (II), zinc (II), and cobalt (II).
Owner:WILLSON RICHARD C +1
Method for separating exosomes from plasma on basis of immobilized metal affinity chromatography
PendingCN110551687AHigh affinitySmall steric hindranceCell dissociation methodsPeptide preparation methodsDiseaseMolecular analysis
The invention provides a method for simply and rapidly separating exosomes or exosome proteins from plasma on the basis of the immobilized metal affinity chromatography. The method at least comprisesthe following steps: 1), the exosomes are adsorbed by monodispersed immobilized affinity chromatographic microspheres CAE-Ti<4+>-IMAC; 2), the exosomes are eluted by ammonium hydroxide; and 3), phosphate buffer is immediately utilized to replace ammonium hydroxide, and damage of ammonium hydroxide to the exosomes is reduced. The collecting method has the characteristics of being low in cost, simple and fast to operate, time-saving, labor-saving and high in collecting efficiency, and besides, various kinds of subsequent molecular analysis are allowed to be performed on the exosomes with the method and include analysis for genomes, proteomes and the like. The problems of high time consumption, high cost, low purity and the like of current exosome separation and purification can be effectively solved, the feasible technical scheme is provided for research of disease plasma exosomes, and the method has good application prospects in the aspect of discovery of new exosome markers and the like.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV
NTA-based (nitrilotriacetate) IMAC (immobilized metal affinity chromatography) filler and preparation method and application thereof
ActiveCN109663384ARealize industrial applicationIon-exchange process apparatusIon-exchanger regenerationMetal affinity chromatographyRaw material
The invention relates to an NTA-based (nitrilotriacetate) IMAC (immobilized metal affinity chromatography) filler and a preparation method and an application thereof. The prepared method of the NTA-based IMAC filler includes the following steps of providing a matrix, constructing an aldehyde group on the surface of the matrix to obtain a matrix with the aldehyde group on the surface, converting the aldehyde group to alpha-aminocarboxylic acid to obtain a matrix with the alpha-aminocarboxylic acid on the surface, constructing an NTA group on the surface of the matrix having the alpha-aminocarboxylic acid on the surface to obtain a matrix with the NTA group on the surface, and chelating the NTA group with transition metal ions to obtain the NTA-based IMAC filler. With the above-mentioned preparation method of the NTA-based IMAC filler, the use of high-cost raw materials is avoided, preparation process is clean, and purification process is simple.
Owner:SEPAX TECH
Method for determination of metal ion adsorption stability parameters on fixed-metal affinity column
The invention discloses an evaluation method for determination of metal ion bonding stability on a fixed-metal affinity chromatography column. The evaluation method comprises preparation of epoxy silica gel, preparation of an amino-carboxylic acid chelating agent and determination of complexation stability constant and saturation adsorption quantity. An amino-carboxylic acid chromatographic column is connected to a chromatogram system, and metal ions of different concentrations are bonded to the amino-carboxylic acid chelating agent through use of frontal chromatography by different buffer solution systems. Breakthrough curves of the metal ions of different concentrations are obtained when bonding balances are achieved, and breakthrough times of the metal ions of different concentrations are calculated through use of Peakfit software. Apparent molar adsorption quantities are calculated based on the breakthrough times. The metal ion concentrations and the apparent molar adsorption quantities are substituted into Langmuir adsorption model, and then the complexation stability constant and the saturation adsorption quantity of fixed metal ions on the amino-carboxylic acid chelating chromatographic column can be determined. The evaluation method is simple and fast, can determine simultaneously multiple adsorption parameters, can more really and accurately reflect the adsorption stability of fixed metal ions on the amino-carboxylic acid chelating agent.
Owner:NORTHWEST UNIV
Introduction of structural affinity handles as a tool in selective nucleic acid separations
InactiveUS20060160093A1Remove pollutantsIncrease effective capacitySugar derivativesMicrobiological testing/measurementGenomic DNAEukaryotic plasmids
The method is used for separating nucleic acids and other similar constructs. It involves selective introduction, enhancement, or stabilization of affinity handles such as single-strandedness in the undesired (or desired) nucleic acids as compared to the usual structure (e.g., double-strandedness) of the desired (or undesired) nucleic acids. The undesired (or desired) nucleic acids are separated from the desired (or undesired) nucleic acids due to capture by methods including but not limited to immobilized metal affinity chromatography, immobilized single-stranded DNA binding (SSB) protein, and immobilized oligonucleotides. The invention is useful to: remove contaminating genomic DNA from plasmid DNA; remove genomic DNA from plasmids, BACs, and similar constructs; selectively separate oligonucleotides and similar DNA fragments from their partner strands; purification of aptamers, (deoxy)-ribozymes and other highly structured nucleic acids; Separation of restriction fragments without using agarose gels; manufacture recombinant Taq polymerase or similar products that are sensitive to host genomic DNA contamination; and other applications
Owner:UNIV HOUSTON SYST +1
Hyperbranched immobilized metal affinity chromatography stationary phase and preparation method thereof
InactiveCN104841404AImprove hydrophilicityGood pollution resistanceOther chemical processesSolid sorbent liquid separationChromatographic separationStationary phase
Owner:NORTHWEST UNIV
Phosphate radical containing cotton modification material and preparation method and application thereof
ActiveCN106637956ANo damage to mechanical propertiesMaintain permeabilityOther chemical processesPeptide preparation methodsCellulosePhosphate
The invention belongs to the field of analytical chemistry. A novel phosphate radical containing cotton modification material is prepared by adopting a graft copolymerization method. The phosphorus content of the material can be well controlled by adjusting the content of a monomer VPA, then metal ions are immobilized on the material, and the material can serve as a material for fixing metal affinity chromatography and used for selective enrichment of phosphorylated polypeptides in multiple biological samples. The optimal material CF-NH2-AZO-p(VPA-2)-Ti4+ can be optimized by researching the relation between the content of phosphate radical groups in the material and material extracting capability. The material has the high selectivity and high extraction capability to the phosphorylated polypeptides in a standard polypeptide mixed solution, milk enzymatic hydrolysate, serum and rat brain lysate. Therefore, application of the graft copolymerization method in cellulose post-modification can be promoted, and application of a cellulose adsorbent in bioanalysis is widened.
Owner:WUHAN UNIV
Human prostate specific antigen expression vector and application thereof
The invention discloses a human prostate specific antigen expression vector and application thereof, which aims to provide the human prostate specific antigen expression vector and a recombinant yeastcell into which the vector is introduced, as well as a method for producing and preparing human prostate specific antigen active fragments by utilizing the recombinant cell. The expression vector isa yeast expression vector containing a human prostate specific antigen gene and a His-tag gene. The method for expressing human prostate specific antigens comprises the steps of constructing the recombinant yeast cell, inducing expression through methanol and utilizing metal affinity chromatography to separate and purify the human prostate specific antigens. The human prostate specific antigen expression vector integrates the human prostate specific antigen gene into a yeast cell genome through homologous recombination to perform stable expression, so that the production cost is greatly reduced.
Owner:上海裕隆生物科技有限公司
Process for the extraction, purification and enzymatic modification of soy 7s globulin alpha' subunit for use as hypocholesterolemizing agent
A process for the extraction, purification and enzymatic modification of β-conglycinin α′ subunit, wherein β-conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the α′ subunit, which is treated with chymotrypsin, then subjected to a further MAC to recover the amino-terminal region of this polypeptide (MW 28,000 Da).
Owner:INDENA SPA
A method for determining the adsorption stability parameters of metal ions on a fixed metal affinity column
Owner:NORTHWEST UNIV
Process for separating and purifying recombinant human serum albumin and fusion protein thereof
ActiveCN102234332BAvoid degradationReduce volumeSerum albuminPeptide preparation methodsBiotechnologyBlood plasma
Different from the conventional method for purifying human serum albumin from human plasma, the invention provides a simple method for separating and purifying the human serum albumin from fermentation liquor or a mammal cell culture solution. By an immobilized metal affinity chromatography (IMAC) step, the method is suitable for purifying recombinant proteins at various salt concentrations, fermentation supernate at high salt concentration is allowed to be directly loaded, the total volume of loading buffer is effectively reduced and the cost is reduced. The method is stable and high-efficiency, and is suitable for laboratories, pilot scale tests and industrial production scale-up.
Owner:ZHEJIANG HISUN PHARMA CO LTD
A high-capacity metal chelate affinity chromatography medium
ActiveCN107754769BLarge specific surface areaLarge fixed areaOther chemical processesSolid sorbent liquid separationMicrosphereAllyl glycidyl ether
The invention relates to a high carrying capacity metal chelating affinity chromatography medium. Silica gel microspheres are adopted as a core; pores are formed inside the silica gel microspheres; carboxymethyl chitosan oligosaccharide molecules are adhered to the surfaces and the pores of the silica gel microspheres; a part of the carboxymethyl chitosan oligosaccharide molecules are complexed with first metal ions to form a first layer of metal chelating; the rest part of the carboxymethyl chitosan oligosaccharide molecules are bonded with allyl glycidyl ether; the allyl glycidyl ether is connected with aglycone; and the aglycone is complexed with second metal ions to form a second layer of metal chelating. By adopting the medium, double metal layers are successfully immobilized on the surface of the medium, the carrying capacity of the metal affinity chromatography medium is effectively increased, and the medium is simple in improvement method, low in production cost and beneficialto large-scale popularization and application.
Owner:SUZHOU BOJIN BIOLOGICAL TECH
A kind of preparation method of gpx7 recombinant protein
InactiveCN104004722BHigh activitySimple stepsOxidoreductasesVector-based foreign material introductionBiotechnologyProkaryotic expression
Owner:上海御先医药科技有限公司
Method for purifying a sulfatase protein
ActiveUS20200361985A1Reduce expensesShorten the timeHydrolasesOrganic anion exchangersChromatographic separationAnion-exchange chromatography
A method for purification of a sulfatase using metal chelating chromatography without using tags such as His-tag, etc. is disclosed. An embodiment provides a method for purifying a sulfatase including the steps of: (a) providing a sulfatase-containing solution comprising one or a plurality of impurities; (b) performing a first chromatographic separation of the sulfatase-containing solution using a metal affinity chromatography resin; (c) performing a second chromatographic separation using a cation exchange chromatography resin; and (d) performing a final chromatographic separation using an anion exchange chromatography resin, wherein the impurities are removed thereby.
Owner:THE GREEN CROSS CORP
Recombinant fibronectin with anti-wrinkle repair function and its preparation method and application
ActiveCN111548410BReduce tensionReduce generationCosmetic preparationsConnective tissue peptidesBinding peptideSkin repair
The invention belongs to the technical field of biology. The invention relates to a recombinant fibronectin with anti-wrinkle repairing functions. The recombinant fibronectin comprises at least one RGD structural domain capable of being combined with a cytointegrin receptor, and at least one sodium ion channel binding peptide fragment capable of acting on a sodium ion channel, the RGD structural domain and the sodium ion channel binding peptide fragment are connected through a polypeptide connecting fragment containing 0-300 amino acid residues, the capability of combining a sodium ion channeland an integrin receptor is realized, the effects of promoting skin repair and metabolic renewal and enhancing freckle fading and wrinkle reducing are achieved, and the process for the preparation thereof, high-density fermentation, super-tolerant metal affinity chromatography purification, specific protease label excision and ion column refining purification methods are adopted. The preparationmethod utilizes high-density fermentation, super-tolerant metal affinity chromatography purification, specific protease excision label and ion column refining purification method. The obtained recombinant fibronectin rFN does not contain a purification label, is high in purity, high in specific activity, simple and efficient in preparation process, low in cost and suitable for large-scale production, and further relates to an application of the recombinant fibronectin rFN in in-vitro adherent cell culture, skin injury repair, wrinkle removal and spot fading.
Owner:江苏费森尤斯医药用品有限公司 +2
Introduction of structural affinity handles as a tool in selective nucleic acid separations
InactiveUS7893222B2Efficiently capture nucleic acidsRemove pollutantsSugar derivativesMicrobiological testing/measurementGenomic DNAPlasmid dna
The method is used for separating nucleic acids and other similar constructs. It involves selective introduction, enhancement, or stabilization of affinity handles such as single-strandedness in the undesired (or desired) nucleic acids as compared to the usual structure (e.g., double-strandedness) of the desired (or undesired) nucleic acids. The undesired (or desired) nucleic acids are separated from the desired (or undesired) nucleic acids due to capture by methods including but not limited to immobilized metal affinity chromatography, immobilized single-stranded DNA binding (SSB) protein, and immobilized oligonucleotides. The invention is useful to: remove contaminating genomic DNA from plasmid DNA; remove genomic DNA from plasmids, BACs, and similar constructs; selectively separate oligonucleotides and similar DNA fragments from their partner strands; purification of aptamers, (deoxy)-ribozymes and other highly structured nucleic acids; Separation of restriction fragments without using agarose gels; manufacture recombinant Taq polymerase or similar products that are sensitive to host genomic DNA contamination; and other applications.
Owner:UNIV HOUSTON SYST +1
A phosphorylated peptide adsorbent and its preparation and application
ActiveCN113318715BImprove thermal stabilityStrong acid and alkali resistanceOther chemical processesSolid sorbent liquid separationMethacrylateFunctional monomer
The invention relates to a phosphorylated peptide adsorbent and its preparation and application. Specifically, trimethylolpropane trimethacrylate (Trimethylolpropane trimethacrylate, TRIM) and allyl glycidyl ether (Allyl glycidyl ether, AGE) are used as functional monomers, and the polymerization with epoxy functional groups is prepared by suspension polymerization. and then modified with O‑phospho‑L‑serine (O‑phospho‑L‑serine) to introduce phosphate groups directly on the surface of the microspheres, and finally with Ti 4+ After ion chelation, it can be used as an adsorbent for immobilized metal affinity chromatography (IMAC) to enrich phosphopeptides in complex biological samples. The preparation method is simple, the raw materials are readily available, the cost is low, the reaction conditions are mild, and the method is suitable for large-scale preparation. The obtained material has stable properties, strong alkali resistance, and high enrichment efficiency and specific selectivity for phosphopeptides in biological samples.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI +1
Kringle 5 gene-containing engineered bacterium fermentation process and kringle 5 protein purification process
InactiveCN101671713AHigh expressionHigh recovery rateMicroorganism based processesPeptide preparation methodsAngiogenesis InhibitionUltraviolet
The invention relates a kringle 5 gene-containing engineered bacterium fermentation process and a kingle 5 protein purification process. In the processes, optimal media suitable for the growth of recombinant engineering bacteria and the expression of a kingle 5 protein are determined, the fermentation conditions of the recombinant bacteria are optimized by an orthogonal experiment method, the fermented kringle 5 protein is purified by a two-step method of metal affinity chromatography and cation-exchange chromatography in high, medium and low chromatographs of a device which has gradient elution and can display ultraviolet ray and conductivity parameters at the same time, and an chicken chorioallantoic membrane angiogenesis inhibition test for activity detection is performed. The processescan obvious improve the expression of kringle and conveniently and quickly purify kringle 5 protein to ensure the kringle 5 meets required standards in both purity and biological activity. The processes are simple in production process and high in yield and can be linearly amplified for industrial large-scale production.
Owner:SHANXI LIFEGEN
Tilapia calcium ion binding peptide as well as preparation method and application thereof
ActiveCN112210579AImprove biological activitySimple processProtein composition from fishPeptide preparation methodsCalcium ion bindingUltrafiltration
The invention discloses a preparation method and application of tilapia calcium ion binding peptide. The preparation method comprises the following steps of pretreating tilapia processing wastes; then, adopting tilapia endogenous enzyme and commercial protease for compounding and enzymolysis; performing ultrafiltration separation; carrying out immobilized metal affinity chromatography; and preparing the active peptide and calcium ion conjugate to obtain the tilapia calcium ion binding peptide. The tilapia calcium ion binding peptide prepared by the method disclosed by the invention has biological activity of promoting calcium ion transport and absorption, and in addition, the preparation method of the tilapia calcium ion binding peptide disclosed by the invention is relatively simple in process and safe in product, and can be applied to actual production.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Process for the purification of tnf-binding proteins using imac
InactiveUS20060128616A1Efficient purificationBig impactPeptide/protein ingredientsImmunoglobulins against cytokines/lymphokines/interferonsTumor necrosis factor binding proteinCopper
A new purification process for Tumor Necrosis Factor-binding proteins is described. In particular this process is characterized by the use as capture step of an Immobilized Metal Affinity Chromatography (IMAC) using copper as metal. This brings advantages in terms of process yields, purity of the final product and applicability to industrial scale.
Owner:ARES TRADING SA
Purification of recombinant human factor XIII
ActiveUS8461302B2Peptide/protein ingredientsMammal material medical ingredientsIon exchangeFactor ii
The present invention provides improved methods for the purification of factor XIII. In particular, the methods provide compositions containing 5% or less contaminating proteins. In particular embodiments of the present invention the methods provide purified factor XIII compositions comprising less than 1% activated factor XIII, less than 2% protein aggregates, and / or less than 5% charge isomers of factor XIII. The methods do not require the use a precipitation or crystallization step common to prior methods of isolating factor XIII. Instead, the method uses immobilized metal affinity chromatography to remove various contaminants common to recombinant expression of factor XIII. Further, a combination of various chromatography methods including ion exchange chromatography, hydrophobic affinity chromatography, and immobilized metal affinity chromatography comprise a simple and less expensive method to produce a pharmaceutical grade factor XIII product at high yield.
Owner:ZYMOGENETICS INC
Hyperbranched immobilized metal affinity chromatography stationary phase and preparation method thereof
InactiveCN104841404BImprove hydrophilicityGood pollution resistanceOther chemical processesSolid sorbent liquid separationStationary phasePhysical chemistry
The invention discloses a hyperbranched fixed metal affinity chromatography stationary phase represented by the general structural formula (I), which uses a hyperbranching method on the surface of the chromatography stationary phase to modify the material surface, thereby increasing the density of functional groups on the surface of the material and improving the Protein has a higher adsorption capacity. The chromatography stationary phase prepared by the invention has metal chelation chromatography separation performance. When it is used to separate lysozyme in egg white, good separation effect and high protein recovery rate can be achieved. .
Owner:NORTHWEST UNIV
Method for preparing recombinant GPx7 (glutathione peroxidase) protein
InactiveCN104004722AHigh activitySimple stepsOxidoreductasesVector-based foreign material introductionBiotechnologyProkaryotic expression
The invention relates to the field of biomedicine and particularly relates to a method for preparing a recombinant GPx7 (glutathione peroxidase) protein. The invention discloses a method for preparing a GPx7 recombinant protein. The method comprises the steps of constructing a GPx7 recombinant protein prokaryotic expression plasmid and strain; expressing the GPx7 recombinant protein; extracting the GPx7 recombinant protein and purifying the GPx7 recombinant protein. The method provided by the invention has the advantages of simple steps, low cost and high degree of purification; the prepared protein has good activity. The method is far superior to a metal affinity chromatography used in the prior art.
Owner:上海御先医药科技有限公司
A kind of cotton modified material containing phosphate radical and its preparation method and application
ActiveCN106637956BNo damage to mechanical propertiesMaintain permeabilityOther chemical processesPeptide preparation methodsCellulosePhosphate
Owner:WUHAN UNIV
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