The invention belongs to the technical field of biology. The invention relates to a recombinant fibronectin with anti-wrinkle repairing functions. The recombinant fibronectin comprises at least one RGD structural domain capable of being combined with a cytointegrin receptor, and at least one sodium ion channel binding peptide fragment capable of acting on a sodium ion channel, the RGD structural domain and the sodium ion channel binding peptide fragment are connected through a polypeptide connecting fragment containing 0-300 amino acid residues, the capability of combining a sodium ion channeland an integrin receptor is realized, the effects of promoting skin repair and metabolic renewal and enhancing freckle fading and wrinkle reducing are achieved, and the process for the preparation thereof, high-density fermentation, super-tolerant metal affinity chromatography purification, specific protease label excision and ion column refining purification methods are adopted. The preparationmethod utilizes high-density fermentation, super-tolerant metal affinity chromatography purification, specific protease excision label and ion column refining purification method. The obtained recombinant fibronectin rFN does not contain a purification label, is high in purity, high in specific activity, simple and efficient in preparation process, low in cost and suitable for large-scale production, and further relates to an application of the recombinant fibronectin rFN in in-vitro adherent cell culture, skin injury repair, wrinkle removal and spot fading.