Tilapia calcium ion binding peptide as well as preparation method and application thereof

A technology of tilapia and calcium ions, which is applied to the preparation methods, applications, chemical instruments and methods of peptides, etc., to achieve the effects of simple preparation process, good calcium chelating ability, and simple process

Active Publication Date: 2021-01-12
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the method of using tilapia proce

Method used

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  • Tilapia calcium ion binding peptide as well as preparation method and application thereof
  • Tilapia calcium ion binding peptide as well as preparation method and application thereof

Examples

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Embodiment 1

[0044] A preparation method of tilapia calcium ion-binding peptide, comprising the following steps:

[0045](1) Soak the minced meat produced after tilapia processing in a NaOH solution with a mass percentage concentration of 0.2wt%, the soaking temperature is 40°C, and the soaking time is 1h, washed with water until neutral, drained and twisted Minced tilapia meat;

[0046] (2) Soak the fish skin produced after tilapia processing in a NaOH solution with a mass percent concentration of 1.5 wt%, the soaking temperature is 35°C, and the soaking time is 3h, washed with water until neutral, drained and twisted Minced tilapia skin and meat;

[0047] (3) Get the tilapia fish intestines, wash with deionized water, and then add a concentration of 0.04mol / L phosphate buffer (pH value is 8.0) to carry out homogenate and break, the tilapia fish intestines and phosphate buffer The mass-to-volume ratio is 1:5g / mL, ultrasonic extraction treatment (ultrasonic frequency is 20kHz, temperatur...

Embodiment 2

[0060] A preparation method of tilapia calcium ion-binding peptide, comprising the following steps:

[0061] (1) Soak the minced meat produced after tilapia processing in a NaOH solution with a mass percentage concentration of 0.3wt%, the soaking temperature is 35°C, and the soaking time is 1.5h, washed with water to neutrality, and drained Grind tilapia minced meat;

[0062] (2) soak the fish skin produced after tilapia processing in the NaOH solution whose mass percent concentration is 1.0wt%, the temperature of immersion is 30 ℃, the time of immersion is 2.5h, wash with water to neutrality, after draining Minced tilapia skin and meat;

[0063] (3) Get the tilapia fish intestines, wash with deionized water, and then add a concentration of 0.05mol / L phosphate buffer (pH value is 8.5) to carry out homogenization and crushing, the tilapia fish intestines and phosphate buffer The mass-to-volume ratio is 1:4g / mL, ultrasonic extraction treatment (ultrasonic frequency is 20kHz, t...

Embodiment 3

[0069] A preparation method of tilapia calcium ion-binding peptide, comprising the following steps:

[0070] (1) Soak the minced meat produced after tilapia processing in a NaOH solution with a mass percentage concentration of 0.5 wt%, the soaking temperature is 50°C, and the soaking time is 2h, washed with water until neutral, drained and twisted Minced tilapia meat;

[0071] (2) Soak the fish skin produced after tilapia processing in a NaOH solution with a mass percentage concentration of 0.5wt%, the soaking temperature is 35°C, and the soaking time is 2.5h, washed with water to neutrality, and drained Minced tilapia skin and meat;

[0072] (3) Get the tilapia fish intestines, wash them with deionized water, and then add a concentration of 0.03mol / L phosphate buffer solution (pH value is 8.5) to carry out homogenization and crushing, the tilapia fish intestines and phosphate buffer solution The mass-to-volume ratio is 1:4g / mL, ultrasonic extraction treatment (ultrasonic fr...

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Abstract

The invention discloses a preparation method and application of tilapia calcium ion binding peptide. The preparation method comprises the following steps of pretreating tilapia processing wastes; then, adopting tilapia endogenous enzyme and commercial protease for compounding and enzymolysis; performing ultrafiltration separation; carrying out immobilized metal affinity chromatography; and preparing the active peptide and calcium ion conjugate to obtain the tilapia calcium ion binding peptide. The tilapia calcium ion binding peptide prepared by the method disclosed by the invention has biological activity of promoting calcium ion transport and absorption, and in addition, the preparation method of the tilapia calcium ion binding peptide disclosed by the invention is relatively simple in process and safe in product, and can be applied to actual production.

Description

technical field [0001] The invention belongs to the technical field of mineral ion-binding peptides, and in particular relates to a tilapia calcium ion-binding peptide and its preparation method and application. Background technique [0002] Peptide-mineral ion conjugate is an organic compound containing mineral ions, which is prepared by chelation reaction between mineral ions and peptides, which can improve the bioavailability of mineral ions by virtue of the absorption mechanism of peptides in the body , with physiological and biochemical properties that inorganic mineral ions do not have. At present, the chelate prepared by chelating essential trace elements and peptides has become a new type of mineral ion supplement, and has attracted more and more attention. [0003] my country is a big producer of tilapia, and its output ranks first in the world. At present, the processing of tilapia is mainly processed into fish fillets. During this process, a large amount of proc...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/22C07K1/113A23L33/18A23L33/16A23J3/34A23J1/04
CPCC12P21/06C07K1/36C07K1/34C07K1/22C07K1/113A23L33/18A23L33/16A23J1/04A23J3/341
Inventor 胡晓李来好杨贤庆陈胜军吴燕燕马海霞邓建朝杨少玲
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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