42 results about "Melanin granule" patented technology

The invention relates to an experimental method for analyzing the toxicity and the effect of a skin whitening agent through a primary culture human being skin melanocyte. The method comprises the following steps: 1, culturing and identifying the primary human being skin melanocyte; 2, carrying out the analysis of the toxicity of skin whitening substances through the primary human being skin melanocyte; and 3, carrying out the analysis of the effect of the skin whitening substances through the primary human being skin melanocyte, namely carrying out the analysis on two aspects of the melanin content and the tyrosinase activity. The method has the following advantages that: the human being melanocyte cultured in vitro has a high division and multiplication capacity, a high standardization degree, little difference between batches, and the same function of producing melanin granules with the melanocyte in vivo; the result obtained through the healthy human being melanocyte is more reliable than the result obtained through animal or human-derived melanoma cells; and the toxic action and the whitening effect of the substances to be tested are evaluated from the two aspects of characterization and quantification. The method can replace the living body animal and human being skin, and can be directly applied to toxicity and effect experiments of the whitening substances in products such as chemicals, cosmetics, medicaments, and the like.

The present invention provides nano-sized particles of melanin, and the method for formation of the melanin particles comprise the following steps: adding a base to a dopamine.H+X−-containing solution (wherein H+X− is an acid) and allowing an acid-base neutralization reaction; and forming nano-sized particles of melanin by controlling the addition of nucleic dopamine.H+X− to base (b) at a ratio of a:b=1:0.1-1, and allow concurrent or consecutive formation of melanin by oxidation curing of the dopamine in air (polymerization). The manufacturing method according to the present invention can produce nano-sized melanin particles in a short period of time. Furthermore, the nano-sized melanin particles made according to the present invention are distinctive from conventional solvents containing dispersions of natural melanin and synthetic melanin, and have excellent application in various fields.

The invention discloses a preparation method for melanin. The preparation method comprises the following steps: adding a melanin precursor, a surfactant and an oxidant into a polymer solution respectively; regulating the pH (Potential of Hydrogen) value to be 7 to 13; and while stirring, carrying out an oxidization reaction to prepare the melanin, wherein the mol ratio of the melanin precursor to the oxidant is 1 to (0.1 to 10), the concentration of a water-soluble polymer is 0.01% to 5% and the concentration of the surfactant is 0.01% to 2%. The melanin prepared by the preparation method has a relatively small particle diameter; and a functional thin film prepared by utilizing melanin granules prepared by the preparation method has a strong ultraviolet shielding function and has high visible light transmittance.

The invention provides a makeup removing composition, a skin care composition and preparation methods. The makeup removing composition is black cream, and comprises the following ingredients in percentage by weight: 1.0-5.0 percent of bamboo charcoal extract, 1.0-3.0 percent of bamboo vinegar extract, 0.1-0.2 percent of hard water softening agent, 15-30 percent of surfactant, 1-5 percent of foaming and thickening agent, 1.0-3.0 percent of greasing agent, 0.1-0.5 percent of preservative, 0.01-0.03 percent of essence and the balance of de-ionized water. The makeup removing composition has strong cleaning power; after the makeup removing composition is used for removing the makeup, the skin is permeable and comfortable, the pores are also clean and relaxed, the formation of melanin granules can be prevented, the metabolism of cells can be accelerated, the activation of aging cells of human bodies can be promoted, the blood circulation can be quickened, and the environments in the human bodies can be improved.

The invention provides an immunohistochemical hematoxylin-toluidine blue double-redyeing method for melanocyte. The method comprises the following steps: (1), carrying out conventional xylene dewaxing on a slice and carrying out gradient alcohol dehydration; (2), blocking and inactivating endogenous peroxide enzyme; (3), repairing an antigen; (4), carrying out serum sealing; (5), dropwise adding primary antibodies; (6) dropwise adding secondary antibodies; (7), carrying out DAB developing; (8), carrying out hematoxylin redyeing and hydrochloric acid alcohol differentiation; (9), redyeing by a toluidine blue working solution and carrying out glacial acetic acid differentiation; (10), dehydrating to be transparent and sealing the slice. According to the invention, positive granules are still brownish yellow after redyeing, and original melanin granules in the tissue become deep green, so that distinction is obvious, the melanin granules are easily distinguished from brown granules, the developing of hematoxylin is not influenced, cell nucleuses of background cells are clearly visible, the color difference of immunohistochemical positive brownish yellow granules, deep green melanin granules and blue cell nucleus is obvious and intuitive, and distinguishing is convenient. Toluidine blue dyeing liquid is prepared simply and conveniently.

The invention belongs to the field of cosmetics and particularly relates to a skin-whitening, anti-aging and anti-allergic hesperidin composition, an application and a preparation. According to the composition containing hesperidin and pectolinarin, provided by the invention, the number of melanin particle-containing positive cells and the content of tyrosinase can be remarkably reduced, the SOD activity can be remarkably improved, the MDA content can be reduced, and the ear swelling and the licking frequency of an experimental animal can be remarkably reduced; and the hesperidin and the pectolinarin are compounded to achieve the effects of remarkably whitening skin, resisting aging and resisting allergy.

The invention discloses a preparation method of melanin particles. The method comprises the following steps: (1) adding an alkali into a water solution containing a melanin precursor, and adjusting the pH value to be 7-13; (2) adding an oxidizing agent into the system, carrying out heating and stirring to carry out an oxidation reaction on the melanin precursor, and simultaneously promoting a self-polymerization reaction; and (3) ending the reaction, adjusting the pH value of the system to be less than or equal to 7, and carrying out centrifugal drying. The melanin precursor used in the preparation method is low in price, each of the prepared melanin particles is of an obvious spherical shape, and the particle size is uniform. A polymer composite material obtained by compounding the prepared melanin particles with polycarbonate and the like has excellent ultraviolet blocking capability and high cost performance.

The invention provides a skincare makeup removing cream and a preparation method thereof.The skincare makeup removing cream is prepared from, by weight, 20-55% of grease, 10-35% of surfactant, 2-30% of solid wax, 1-5% of bamboo charcoal extract liquor, 1-3% of bamboo vinegar extract liquid, 0.4-2% of Chinese herbal medicine extract, 0.1-0.2% of methylparaben, phenoxyethanol or imidazolidinyl urea, 0.01-0.03% of essences and the balance deionized water.Due to addition of the Chinese herbal medicine extract, a function of makeup removing can be realized while skincare efficacies of skin tightening, pore shrinking, acne removing, wrinkle smoothing, bacterium killing and the like are achieved.Due to addition of the bamboo charcoal and bamboo vinegar extracts which are purely natural products, nontoxic, odorless, free of side effects, mild and nonirritating, the skincare makeup removing cream is excellent in cleansing performance, enables skin permeability and comfort, pore cleanness and freeness of tightness and is capable of preventing melanin granules, accelerating cell metabolism and promoting activation of senile cells of human bodies.

The invention relates to a method for preparing a zirconium silicate-coated black material, which comprises the following steps: (1) uniformly mixing melanin particles, zirconium silicate powder and flux powder to form a mixture; (2) using microwaves to mix The material is heated until the zirconium silicate powder melts into a liquid phase and is uniformly wrapped on the outside of each melanin particle; (3) annealing. The present invention makes full use of the characteristics of melanin particles such as graphite powder, plant carbonized carbon black, and organic matter conversion carbon black that can fully absorb microwave heat, changes the lattice characteristics of melanin particles, increases the surface activity of melanin particles, and uses flux to assist in melting silicon Zirconium acid, forming a liquid phase to realize the zirconium silicate wrapping of melanin, forming a denser, stronger adhesion transparent composite layer, the quality of the black pigment is better, only by adding flux for microwave heating treatment, the process only It takes tens of minutes, and the process is simple, the cost of raw materials is low, energy saving and environmental protection, and it is suitable for industrialized production.

The invention relates to a preparation method of cuttlefish ink nanometer particles. The preparation method is characterized by comprising the following steps of I, pretreating cuttlefish ink sacs toobtain cuttlefish ink melanin crude products; II, performing enzymolysis on the cuttlefish ink melanin crude products to obtain cuttlefish juice after enzymolysis, and storing the cuttlefish juice after enzymolysis at 3-7 DEG C for standby application; III, performing dynamic superhigh pressure micro jet treatment: putting the cuttlefish juice after enzymolysis in a high-pressure micro jet homogenizing machine, and performing dynamic superhigh pressure micro jet treatment and circulation once at the pressure of 40-200MPa, so as to obtain refined cuttlefish juice; and IV, performing vacuum freeze drying so as to obtain the cuttlefish ink nanometer particles. The invention also discloses an application of the cuttlefish ink nanometer particles. Compared with the prior art, the preparation method disclosed by the invention has the advantages that cuttlefish ink melanin particles of which the uniformity, the solubility and the fine and smooth properties in mouth feel are improved can be obtained, and the cuttlefish ink melanin particles can be applied to foods.

The invention discloses a hair blacking essence capable of changing white hair into black hair and a preparation method thereof. The hair blacking essence capable of changing white hair into black hair comprises the following components in parts by mass of 76-79 parts of an agent A, 15.6-17.7 parts of an agent B, 0.55-1 part of an agent C and 2.75-3 parts of an agent D, wherein the agent B is prepared from cocamide MEA, sodium methyl 2-sulfolaurate and a plant extract; the preparation method comprises the steps of raising the temperature of the agent A to 80-90 DEG C; adding the agent B into the agent A, and stirring at constant temperature for 13-20 minutes; then adding the agent C, and stirring for 28-32 minutes at constant temperature; and cooling to 45 DEG C or below, keeping the temperature constant, adding the agent D into the mixture, and stirring for 25-35 minutes. All the traditional Chinese medicines coordinate and promote one another, formation of melanin particles is promoted, a transport channel is unblocked, synthesis and transfer of primary pigment cells are promoted, and the activity of tyrosinase is enhanced, so that the white hair contains melanin, and the effectof blackening from white is achieved.

The invention provides a method for preparing a hop whitening extract, which relates to the field of cosmetic production. According to the invention, a simple and rapid method is used to extract an extract product with skin whitening activity from hops, and the experiments prove that the effective component can significantly reduce the melanin content and inhibit the transport of melanin particlesto keratinocytes. The preparation method comprises the following steps: pulverizing the hops after drying, adding 95% alcohol for extraction, extracting the obtained extract and adding petroleum ether for degreasing, extracting the active ingredient by adding ethyl acetate, decolouring the material by macroporous adsorption resin, and performing freeze drying to obtain a hop whitening effective component extract; adding 1,3-butanediol and deionized water, and filtering the material through a 0.22 [mu]m filter membrane to obtain the hop whitening extract.

The present invention provides nano-sized particles of melanin, and the method for formation of the melanin particles comprise the following steps: adding a base to a dopamine.H+X−-containing solution (wherein H+X− is an acid) and allowing an acid-base neutralization reaction; and forming nano-sized particles of melanin by controlling the addition of nucleic dopamine.H+X− (a) to base (b) at a ratio of a:b=1:0.1-1, and allow concurrent or consecutive formation of melanin by oxidation curing of the dopamine in air (polymerization). The manufacturing method according to the present invention can produce nano-sized melanin particles in a short period of time. Furthermore, the nano-sized melanin particles made according to the present invention are distinctive from conventional solvents containing dispersions of natural melanin and synthetic melanin, and have excellent application in various fields.

The present invention relates to a moisturizing, hydrating and whitening emulsion and a preparation method thereof. Glabridin, L-ascorbic acid-glucoside and vitamin E are added, the multiple components have synergistic effects, glabridin usage is reduced, tyrosinase activity inhibition rate is increased and reaches 97.27%, SOD, L-ascorbic acid-glucoside and vitamin E achieve the synergistic effects, free radicals are removed, melanin formation is reduced, nicotinamide is added to inhibit melanin particles from being transmitted to keratinocytes and increase skin renewal speed, the skin is whitened in multiple ways, and the effects are remarkable; and palmitoyl pentapeptide-3 is added to stimulate collagen, elastic fiber and hyaluronic acid proliferation, so that water content and water locking degree of the skin are improved, the skin thickness is increased, and fine wrinkles are reduced. Coconut oil, small molecular hyaluronic acid, ceramide, butanediol and glycerin are added for moisturizing and hydrating. The product does not contain essence or preservatives, contains few components, is safe and does not easily cause allergy.

The invention discloses an anti-ultraviolet and heat-resistant natural raw lacquer film. The anti-ultraviolet and heat-resistant natural raw lacquer film comprises melanin particles and a natural rawlacquer. The defect of poor ultraviolet resistance of traditional natural raw lacquer is overcome by utilizing the characteristic that THE melanin particles absorb ultraviolet rays, so that the heat-resistant natural raw lacquer film with good ultraviolet resistance is obtained.

The invention discloses a pure natural hair blacking and hair care composition and application of the pure natural hair blacking and hair care composition. The pure natural hair blacking and hair carecomposition mainly comprises a cinnamon bark extract, a cupressus sempervirens leaf extract, and kigelia africana. The cinnamon bark extract and the cupressus sempervirens leaf extract are used to becompounded with the kigelia africana extract, the composition has the effects of promoting blood circulation, benefiting qi, nourishing blood, promoting circulation, removing stasis, and clearing andactivating the channels and collaterals, and improves the microcirculation of the blood capillary on the skin layer of the scalp, the pore becomes smooth, the absorption of the nutrient substance bythe scalp is promoted, the normal development and the normal growth period of the hair follicle are recovered, the hair loss and the new hair recover the dynamic balance, and the growth of the hair ispromoted; and the scalp cell is activated, the circulation of blood in the hair papilla is promoted, sufficient nutrients are provided for the hair papilla and the hair bulb, the melanin granule is promoted to be synthesized smoothly, and the white hair becomes the black hair.

The invention relates to an experimental method for analyzing the toxicity and the effect of a skin whitening agent through a primary culture human being skin melanocyte. The method comprises the following steps: 1, culturing and identifying the primary human being skin melanocyte; 2, carrying out the analysis of the toxicity of skin whitening substances through the primary human being skin melanocyte; and 3, carrying out the analysis of the effect of the skin whitening substances through the primary human being skin melanocyte, namely carrying out the analysis on two aspects of the melanin content and the tyrosinase activity. The method has the following advantages that: the human being melanocyte cultured in vitro has a high division and multiplication capacity, a high standardization degree, little difference between batches, and the same function of producing melanin granules with the melanocyte in vivo; the result obtained through the healthy human being melanocyte is more reliable than the result obtained through animal or human-derived melanoma cells; and the toxic action and the whitening effect of the substances to be tested are evaluated from the two aspects of characterization and quantification. The method can replace the living body animal and human being skin, and can be directly applied to toxicity and effect experiments of the whitening substances in products such as chemicals, cosmetics, medicaments, and the like.

The invention discloses hair-blackening shampoo. By adding active ingredients of rosmarinic acid, a Chinese angelica extract product, a polyghace seche extract product and a curcuma tuber extract product into the shampoo, the shampoo is natural, safe and effective. The rosmarinic acid can promote expression of tyrosinase and induce generation of melanin; the Chinese angelica extract product can improve scalp microcirculation and promote scalp to absorb nutrients and also can provide the hair with rich trace elements; the polyghace seche extract product can activate tyrosinase activity and promote generation of melanin; and the curcuma tuber extract product has effects of invigorating blood circulation and promoting qi circulation and relieving depression. With synergism of the active ingredients, the shampoo can promote and activate blood circulation, promote transdermal absorption, improve scalp health, help effective active ingredients to rapidly reach the hair core, hair root and hair follicle, supplement effective ingredients essential to hair, activate tyrosinase activity, promote generation of melanin grains, blacken the hair and make the hair to be glossy.

The invention provides whitening buccal tablets containing SOD components. The whitening buccal tablets consist of the following raw materials in percentage by weight of 0.5-1% of SOD, 5-10% of resveratrol, 5-10% of nicotinamide, 5-10% of vitamin C, 10-12% of starch slurry, 1-2% of a lubricant, 30-40% of microcrystalline cellulose and 20-32% of a correctant. The SOD is added, so that free radicalscan be eliminated, and formation of melanin is reduced; the resveratrol is added, so that the activity of tyrosinase can be restrained, and the formation of the melanin can be reduced; the nicotinamide is added, so that transmission of melanin granules to horn cells can be restrained, and the skin renewal rate can be increased; the vitamin C is added, so that melanin intermediates and melanin polymers are reduced, skin and muscle are whitened in various ways, effects are notable, and the components are safe. The dissolving time is between 18.53-22.60min, so that the absorption time of the antioxidant passing through the oral mucosa can be guaranteed, and the situations that the time is too long and the whitening buccal tablets are inconvenient to use cannot be caused.

The invention provides a method for extracting melanin from Stizolobium capitatum seed coats. The method comprises the steps: carrying out an extractive process: subjecting the Stizolobium capitatum seed coats to water cooking so as to remove impurities, then, adding the Stizolobium capitatum seed coats into a NaOH solution in accordance with that the material / liquid ratio is 1g: (6 to 9)ml, carrying out extraction for 10 to 14 hours in a nitrogen gas environment, then, adding a HCl solution into the solution so as to adjust the pH to be smaller than 2.5, and carrying out centrifugal collecting, so as to obtain gelatinous precipitates; carrying out a purifying process: suspending the collected gelatinous precipitates in a HCl solution, of which the volume is 15 to 25 times that of the gelatinous precipitates, carrying out hydrolysis in boiling water bath for 2 to 6 hours, carrying out cooling, and then, carrying out centrifugal collecting, so as to obtain solid granules; washing the collected solid granules with ethanol, ethyl acetate and acetone sequentially, and then, carrying out repeated precipitation by using the NaOH solution and the HCL solution, thereby obtaining melanin granules. According to the method, the operation is simple, the ratio of extraction of the melanin from the Stizolobium capitatum seed coats is high, the purity of the melanin is high, the degree of oxidation of the melanin is low, and the extraction cycle is short; in addition, the cost is low, so that the method is suitable for being used for carrying out large-scale extraction on the melanin of the Stizolobium capitatum seed coats; the economic value of Stizolobium capitatum is increased, and the field of application of the Stizolobium capitatum is widened.

The invention provides an extraction method of banana peel melanin. The extraction method comprises an extraction process and a purification process, wherein the extraction process comprises the steps of boiling banana peel with water, removing impurities, adding a NaOH solution being 1-3 times of volume of the banana peel for extracting for 8-12 minutes, then adding an HCl solution for regulating pH to be smaller than 2.5, centrifuging and collecting to obtain gelatinous precipitate. The purification process comprises the steps of suspending the collected gelatinous precipitate in 15-25 times of volume of HCl solution, hydrolyzing in boiled water bath for 8-12 hours, cooling, then centrifuging and collecting solid particles; successively washing the collected solid particles by using ethyl alcohol, ethyl acetate and acetone in sequence, then precipitating repeatedly by using the NaOH solution and the HCl solution to obtain melanin particles. The extraction method of banana peel melanin is simple to operate; the banana peel melanin is high in extraction amount and high in purity; the melanin oxidation degree is low; the extraction period is short; in addition, the cost is low; the method is applicable to large-scale extraction of the banana peel melanin; the economic value of the banana peel is improved; the application field of the banana peel is broadened.

The invention belongs to the field of cosmetics and particularly relates to a skin-whitening, anti-aging and anti-allergic hesperidin composition, an application and a preparation. According to the composition containing hesperidin and pectolinarin, provided by the invention, the number of melanin particle-containing positive cells and the content of tyrosinase can be remarkably reduced, the SOD activity can be remarkably improved, the MDA content can be reduced, and the ear swelling and the licking frequency of an experimental animal can be remarkably reduced; and the hesperidin and the pectolinarin are compounded to achieve the effects of remarkably whitening skin, resisting aging and resisting allergy.

The invention is applicable to the technical field of medical equipment, and provides ultrasonic leukotrichia diagnosis and treatment equipment and a computer readable storage medium. The equipment comprises at least one ultrasonic transducer and a control device. The control device is used for driving the first ultrasonic transducer in the at least one ultrasonic transducer to perform ultrasonicimaging to obtain a first scalp image of target scalp, determining ultrasonic parameters according to the first scalp image, and driving the second ultrasonic transducer in the at least one ultrasonictransducer to output an ultrasonic stimulation signal according to the ultrasonic parameters, wherein the ultrasonic stimulation signal is used for stimulating the target scalp. Therefore, an ultrasonic imaging technology and an ultrasonic stimulation technology are fused, tissues at the target scalp can be accurately regulated and controlled by ultrasonic waves to increase melanin particles in hair medulla and cortex, and the purpose of turning white hairs into black hairs is achieved.

The invention relates to a melanin separation and extraction method based on ionic liquid, and belongs to the technical field of waste hair recycling. The method comprises the following steps: depolymerizing hair by using an ionic liquid and a depolymerizing agent, separating amino acid, the ionic liquid and melanin in one step by using an aqueous two-phase system, extracting the amino acid in a phase-splitting agent, and recovering the ionic liquid and the phase-splitting agent. Actual operation steps are simple, high-purity melanin can be efficiently separated, melanin particles keep the original appearance, and follow-up high-end application is facilitated; and main reagents can be recovered and reutilized, so that the production cost can be greatly reduced. The method has the advantages of high efficiency, no emission of three wastes, excellent economic feasibility and the like.

The invention discloses a water-soluble lachnum melanin and an application thereof in preparation of lead-removing promoting medicines. The water-soluble lachnum melanin is characterized by being prepared by modifying the intracellular melanin produced by the lachnum with the strain preservation number of CCTCC No:M2011054 via chloroacetic acid. According to the pharmacological action experiment for promotion of lead removal from a rat infected with the lead, the result shows that the water-soluble lachnum melanin has an obvious lead-removing promoting function and cannot generate toxic and side effects, so that the side effect defects that certain essential elements such as Zn, Cu, Fe and the like in vivo are lost and certain organs are damaged, which are caused by the existing western medicine during the lead removing, are overcome. Thus, the lead-removing promoting function of the water-soluble lachnum melanin is ensured. The method disclosed by the invention is simple and economical. The water-soluble lachnum melanin can be applied to the preparation of the tablet-type or capsule-type lead-removing promoting medicines for oral administration.

The invention discloses montmorillonite-melanin composite haemostatic powder and a preparation method and application thereof.The montmorillonite-melanin composite haemostatic powder is formed by uniformly dispersing and coating natural melanin particles on the surfaces of montmorillonite particles, and surface groups and charges of the montmorillonite particles and the natural melanin particles can be in contact with each other, so that the surface of the montmorillonite particles and the natural melanin particles can be in contact with each other; platelet activation and blood coagulation cascade reaction activation are realized, so that the hemostasis process is accelerated, and the hemostasis performance of the composite material is synergistically and remarkably improved. The montmorillonite-melanin composite hemostatic powder disclosed by the invention is simple in preparation process, the montmorillonite particles and the natural melanin particles have synergistic efficient hemostatic performance, and meanwhile, the montmorillonite-melanin composite hemostatic powder is endowed with good biological safety due to low-toxicity components of the montmorillonite-melanin composite hemostatic powder.

The invention provides a pure natural hair dyeing powder and application thereof, and solves the problems that: an existing hair dyeing product is long in hair dyeing time and repeatedly dyed, and dyeing components are harmful to a human body. According to the technical scheme, the pure natural hair dyeing powder comprises a first agent and a second agent, and the first agent comprises a hair dyeing permeation promoter, dopaquinone and a coloring catalyst; the second agent is a hair dyeing permeation promoter; and the hair dyeing penetration promoter comprises hollyhock, kelp powder, papaya extract, pineapple extract, mung bean extract, a pure natural penetration promoter, a humectant and a pH regulator. The dopaquinone is used as a dyeing agent, oxygen in the environment is used for oxidative polymerization reaction, macromolecular melanin particles are finally formed and embedded into hair cortex, white hair is naturally black, use of strong oxidant hydrogen peroxide and ammonia water with pungent smell is avoided, and scalp health is facilitated.

The invention discloses a halloysite-melanin surface co-deposition hemostatic bandage and a preparation method thereof. The halloysite-melanin surface co-deposition hemostatic bandage is formed by sequentially co-depositing natural melanin particles and halloysite nanotubes on the surface of a hemostatic bandage base material. According to the halloysite-melanin surface co-deposition hemostasis bandage, the natural melanin particles and the halloysite nanotubes are co-deposited on the surface of the hemostasis bandage base material in sequence only through simple dipping, the halloysite-melanin surface co-deposition hemostasis bandage is prepared, the preparation process is simple, the cost is low, and the application range is wide. The halloysite nanotubes and the natural melanin particles have synergistic efficient hemostatic performance, and have good biological safety.