35 results about "M2 phenotype" patented technology

The invention belongs to the technical field of medicines, and in particular discloses application of fructose 1,6-diphosphate (FDP) in preparation of a fetus protection medicine for spontaneous abortion. In vitro and in vivo animal experiments show that the concentration of the FDP in peripheral blood and decidual tissues of patients with recurrent spontaneous abortion is decreased compared withnormal pregnancy. Compared with control pregnant mice, the model plasma and uterus FDP levels of pregnant mice with spontaneous abortion are reduced, the uterus macrophage M2 phenotype molecule expression of the pregnant mice with spontaneous abortion is decreased, the proportions of helper cells T(Th)2 and regulatory T cells are reduced, and the phenomena of poor endometrium decidualization and decreased embryonic trophoblast infiltration degree occur in an accompanied way. The results indicate that the supplement of the FDP can significantly improve the uterine immune tolerance pattern, decidualization and embryonic trophoblast infiltration in the pregnant mice with spontaneous abortion, and obviously reduces embryonic loss in the pregnant mice. Therefore, the FDP is expected to be an early-warning and diagnostic indicator for the patients with spontaneous abortion, and can be used for preparing the fetus protection medicine for the spontaneous abortion.

Embodiments of the present invention describe a method of changing the phenotype of monocytes and macrophages from a proinflammatory M1 phenotype to an anti-inflammatory M2 phenotype. The method can comprises providing a composition comprising a recombinant adeno-associated virus (rAAV) vector comprising an exogenous gene encoding ApoA-I Milano or a fragment thereof, and administering the composition to a mammal in need thereof to change the phenotype of monocytes or macrophages from a proinflammatory M1 phenotype to an anti-inflammatory M2 phenotype. By changing the phenotype of monocytes or macrophages from a proinflammatory M1 phenotype to an anti-inflammatory M2 phenotype, atherosclerosis can be treated. The present invention also describes a method of monitoring macrophage phenotypic switching and a method of assessing the efficacy of the treatment of atherosclerosis.

A therapeutic effect of Nurr1 and Foxa2 in inflammatory neurologic disorders by M1-to-M2 polarization of glial cells is provided. Specifically, a method of converting glial cells from an M1 phenotype to an M2 phenotype, wherein Nurr1 and Foxa2 are introduced into the glial cells to be overexpressed in the glial cells and a method of preventing or treating an inflammatory neurologic disorder, which includes glial cells into which Nurr1 and Foxa2 are introduced, or a viral vector loaded with Nurr1 and Foxa2, are provided.

The invention discloses the application of long-chain non-coding RNA to regulate macrophage polarization in viral myocarditis. The applicant found that lncRNA AK085865 had the most significant differential expression in M1 / M2 macrophages, and the expression level of AK085865 in M2 macrophages was higher than that in M1 macrophages. Downregulation of the AK085865 gene reduces the phenotypic expression of M2 while promoting the polarization of the M1 phenotype; while AK085865 ‑ / ‑ Knockout mice have increased susceptibility to CVB3-induced VM. in AK085865 ‑ / ‑ In the gene knockout VM model mice, the number of M1 macrophages was significantly increased, while the number of M2 cells was decreased. AK085865 specifically interacted with interleukin enhancer-binding factor 2 and was involved in the processing of microRNAs mediated by the ILF2 / ILF3 complex It plays a negative regulatory role in the pathway and promotes the polarization of M2 macrophages.

The invention provides an osteoinductive and immune double-effect coating, a preparation method and an application in osseointegration. The method provided by the invention is to design a double-effect coated bone implant with immunoregulatory activity and osteoinduction by means of mussel molecule-mediated metal-phenol coordination chemistry and bio-orthogonal reaction strategy. Immunologically active Zn via mussel molecular surface adhesion chemistry, ion coordination, and bioorthogonal reactions 2+ Co-modified the surface of bone implants with osteoinductive BMP‑2 peptides, and found that the Zn / BMP‑2 dual-effect coating can better improve the biocompatibility of bone implants and promote macrophages to anti-inflammatory Polarization of the M2 phenotype, synergistically improving the immune microenvironment at the bone-implant interface induces optimal osteogenic properties and osseointegration effects at the bone-implant interface, thereby enhancing mechanical stability in vivo.

The invention discloses a preparation method and application of a wound dressing based on breast milk. The invention provides a method for preparing a wound dressing based on breast milk, comprising the following steps: S1. after freeze-drying the breast milk, dissolve it in a biocompatible solvent to obtain a breast milk solution; wherein, the concentration of the breast milk solution described in step S1 greater than or equal to 1 μg / mL; S2. filter and sterilize the breast milk solution obtained in step S1 to obtain the wound dressing. The wound dressing has low immunogenicity and will not cause additional inflammatory reactions in the body; it can scavenge hydroxyl free radicals and intracellular reactive oxygen species, increase the proliferation rate of cells, promote the migration of fibroblasts and endothelial cells, and strengthen endothelial Cell angiogenesis; regulates the M2 phenotype of macrophages, promotes hair follicle regeneration, and normalizes wound healing. In addition, the material of the wound dressing is derived from food breast milk, which is green and safe, and the preparation process is simple, so it has broad prospects for popularization and application.