High-throughput drug screening system for inhibiting survival of breast cancer cells stimulated by macrophages

Abstract

The invention relates to the field of biomedicines and discloses a high-throughput drug screening system for inhibiting the survival of breast cancer cells stimulated by macrophages. The high-throughput drug screening system comprises 1) preliminary screening: performing mixed culture on the macrophages and the breast cancer cells and analyzing the inhibitory effect of drugs on the activities of the breast cancer cells stimulated by the macrophages to obtain primary screening positive drugs; 2) secondary screening: adding the primary screening positive drugs in the individually-cultured macrophages and breast cancer cells, and analyzing and judging to screen a drug capable of reshaping TAM from an M2 phenotype to an M1 phenotype or interfering / blocking a signal loop between the breast cancer cells and the macrophages. The screening system disclosed by the invention can screen the drug capable of reshaping the macrophages to the M1 phenotype or interfering / blocking the signal loop between the breast cancer cells and the macrophages through individual culture and common culture of the mouse macrophages RAW264.7 and the mouse three-negative breast cancer cells 4T1.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a high-throughput drug screening system for inhibiting the survival of breast cancer cells stimulated by macrophages. Background technique [0002] In 2015, an estimated 332,420 new cases of female breast cancer were diagnosed in the United States, of which 69.7% were diagnosed with invasive breast cancer (malignant), and 60,290 patients died from the disease. Most cancers are aggressive (metastatic cancers), where the cancer cells grow into surrounding tissue and often end up invading other organs, including the liver, lungs, and brain. Survival rates for patients diagnosed with metastatic breast cancer are poor due to the lack of effective curative therapies. Treatment for metastatic breast cancer currently focuses more on quality of life than survival time. [0003] Many studies have shown that the tumor microenvironment (TME) will regulate tumor progression and metastasis, thus p...

AI Technical Summary

Problems solved by technology

At present, the research on the plasticity of macrophages and the mutual conversion mechanism between M1 and M2 phenotypes is still unclear. More mature research is that tumor cells secrete CSF-1, and macrophages secrete EGF, which will promote growth and proliferation each other

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