42 results about "Lymphocyte Numbers" patented technology

The invention discloses a compound probiotic agent for improving immunity of dogs and reducing incidence of diarrhea as well as a preparation method and application thereof, and belongs to the field of the compound probiotic agent. The compound probiotic agent is prepared by mixing and compounding a lactobacillus plantarum KT-Lp9 microbial agent, a lactobacillus casei zhang microbial agent, a lactobacillus plantarum P-8 microbial agent, a bifidobacterium animalis subsp. lactis V9 microbial agent and a dilution carrier, wherein the quantity of viable bacteria of the four kinds of microbial agents is more than or equal to 2*10<11>CFU / g. The compound probiotic agent with different contents of viable bacteria only can be applied to 96 dogs at different ages for 60 days, so that the immunity of the test dogs can be improved, and the incidence of diarrhea and the sickness degree are reduced. Main representations are that the feed intake and growth rate of the dogs are increased, leukocyte counts, lymphocytes counts and neutrophil counts in blood are controlled, and the contents of four immunologic factors including immune globulin G, interleukin 6, interferon alpha and tumor necrosis factor alpha in the blood can be increased; and meanwhile, the content of secretory immunoglobulin A (SigA) in excrement is increased.

The present disclosure provides methods for measuring the number of CD4+ OX40+ Foxp3+ lymphocytes in a sample containing cancer cells and lymphocytes obtained from a subject by labeling lymphocytes that show CD4 expression in the sample, then labeling lymphocytes that show OX40 expression in the sample, then labeling lymphocytes that show Foxp3 expression in the sample, then measuring the number of CD4+ OX40+ Foxp3+ lymphocytes in the sample. Further provided are methods for determining the prognosis of a subject, predicting responsiveness of a subject having cancer to an OX40 agonist treatment, and methods for treating or delaying progression of cancer based on the number of CD4+ OX40+ Foxp3+ lymphocytes in a sample.

The invention belongs to the technical field of cell marking and particularly relates to a GFP-CD19 fusion protein, a preparation method and application of the GFP-CD19 fusion protein in the cell marking aspect. The GFP-CD19 fusion protein comprises 776 amino acid, and the molecular weight is about 85 KD; the fusion protein is used for marking the cells with anti-CD19 bodies expressed on the surfaces. A specific combination principle of antigen-antibody or receptor-ligand is used, the GFP-CD19 fusion protein is specifically prepared, the protein can be specifically combined with a chimeric antigen receptor and is subjected to fluorescent marking, only T-lymphocyte which is effectively transfected and embedded with an antigen receptor can be combined with the protein so that the number of the effectively transfected T cells can be calculated by detecting the number of fluorescent cells, the number of effective T-lymphocyte returned to a patient body can be accurately controlled according to the data, and accordingly, guarantee is provided for treating the tumor with the CAR-T technology.

The present invention relates to a kind of composition containing plant lactobacillus LP-Onlly strain and its application in strengthening intestinal immunobarrier. The composition of the present invention can intervene the homing of intestinal lymphocyte, control the number of intestinal tract secretive IgA and intestinal lymphocyte, lower the endotoxin level, improve intestinal tract immunobarrier and prevent damage of intestinal tract immunobarrier caused by hunger, malnutrition, infection, etc, and prevent and treat intestinal inflammation, infectious diseases and immune disorder diseases.

The invention discloses an enzyme-linked immunospot test method for T lymphocytes. The method comprises the following steps: by adopting a T-SPOT.TB kit produced in British Oxford Immunotec Company, taking 5ml of sodium citrate anticoagulation venous blood, separating mononuclear cells of peripheral blood and placing the mononuclear cells on a gamma-interferon antibody coated porous plate, adding an early secretory antigenic target (ESAT-6) of tuberculosis specific antigen and a culture filtrate protein (CFP-10) to serve as stimulus antigens, culturing in an incubator for 24 hours, washing off T lymphocytes with antigen sensitization effects, adding a biotin labeled second antibody, performing enzyme linked colorimetry, forming spots at secreting positions of cell factors, and detecting the number of the T lymphocytes with the tuberculosis specific antigen sensitization effects by recording the spot number. The method disclosed by the invention contributes to early screening of culture negative pulmonary tuberculosis.

The invention discloses a feed additive capable of improving an intestinal function of grass carps and application of the feed additive. An effective component of the additive is glycylglutamine; and the adding amount in grass carp feed is 0.25-0.75%. With the adoption of the additive disclosed by the invention, the growth and fat metabolism of the grass carps can be effectively facilitated, and the oxidation resistance of the grass carps is improved; a shape structure of small intestines of the grass carps can be improved; villus height, crypt depth, mucous membrane thickness, the quantity of goblet cells and the quantity of lymphocytes of the intestines are increased; and the secretion of an intestinal digestive enzyme can be accelerated, and the feed additive has active improvement effect on the intestinal function of the grass carps.

The invention provides a compound feed additive for enhancing broiler chicken immunity. The compound feed additive is prepared by 28-50 parts of rhodiola glucoside, 15-27 parts of hydrochloric acid berbamine, 9-22 parts of pueraria root extract, 7-20 parts of schisanhenol and 6-16 parts of eupolyphaga powder. The compound feed additive can effectively promote growth of broiler chicken immune organs, obviously improve the level of immune globulin IgG in serum, the lymphocyte transformation rate and the leukocyte phagocytic ability, increase the number of T-lymphocytes and enhance the body liquid and the cellular immune function of broiler chickens, and therefore the incidence rate of diseases and the use frequency of antibiotic and other medicine are reduced, and direct economic and social benefits are achieved.

The invention relates to the field of cell proliferation detection and a method and a kit for detecting lymphocyte proliferation conditions through a non-diagnostic purpose. The method comprises the following steps: mixing a first to-be-detected blood sample with a lymphocyte polyclonal activator, and performing cell culture, thereby obtaining first blood cells; incubating a fluorescence-labeled lymphocyte surface labeled antibody with the first blood cells, performing red cell lysis, adding microspheres for absolute counting, detecting the number of lymphocytes and the number of the microspheres for absolute counting by adopting flow cytometry, and obtaining the concentration of lymphocytes in a stimulation group according to the number of lymphocytes, the number of the microspheres for absolute counting and the concentration of the microspheres for absolute counting. The proliferation ratio of each subgroup of the lymphocytes can be detected, the absolute value of proliferation of each subgroup of the lymphocytes also can be detected, and the proliferation conditions of the lymphocytes can be accurately reflected; a proliferation experiment is performed by using whole blood, PBMC does not need to be separated, and the requirement on the amount of the blood sample is small; and moreover, the operating steps are simple, the time consumption is low, and the error is small.

The invention provides a method for separating lymphocytes from a leukocyte removal filter. The method comprises the following steps: obtaining mixed liquid of PBS (Phosphate Buffered Saline), leukocytes and a part of blood; discarding plasma, adding the phosphate buffered saline to the cell mixed liquid without the plasma, and carefully and uniformly mixing so as to form mixed liquid; and carefully superimposing the mixed liquid on cell separating liquid, centrifuging, collecting a second layer of cells, putting in the phosphate buffered saline, fully and uniformly mixing, centrifuging, discharging supernatant, remaining precipitates, then repeatedly washing, and finally precipitating so as to obtain separated peripheral blood mononuclear cells; and culturing the separated peripheral blood mononuclear cells in a 37-DEG C incubator with 5% of CO2, cultivating overnight, and collecting the suspended lymphocytes. The method for separating the lymphocytes from the leukocyte removal filter, which is provided by the invention, is capable of avoiding the wasting of the lymphocytes and has no influence on other components such as red blood cells and blood platelets; the number of the lymphocytes obtained by using the separation method can reach 1*10<6>, and the survival rate of the lymphocytes is greater than 90%.

A method to characterize a T-cell response of a final population of T-lymphocytes resulting from the co-incubation of an initial population of T lymphocytes with a composition of antigen-presenting cells (APCs), said method comprising the steps of a) simultaneous measuring on a single cell basis at least two parameters: (i) proliferation of T lymphocytes and (ii) presence of a T cell antigen receptor on the surface of T lymphocytes and / or presence of at least one biological molecule produced by T lymphocytes, and the attribution of a positive or a negative value to each of these parameters, and b) classification of the final T-lymphocytes population into 2n different subsets of T lymphocytes, n being the number of parameters, each subset being characterized by a positive or a negative value respectively to each parameter and the determination of the proportion of T lymphocytes present in each subset with respect to the number of T lymphocytes in the final population, with said proportion being characteristic of the T-cell response. Use of the method for batch release assay and potency assay.

This invention discloses a detecting method for immune status of T-cell of hepatitis B patients. It is to use artificial synthesized polypeptide to stimulate the peripheral blood lymphocyte cultivated out of hepatitis B patients; to detect the number of lymphocyte with Gamma interferon in simulated body by use of enzyme associated immune blob experimental method, so to determine immune status of T-cell of hepatitis B patients. In normal matched group, there is barely no non-specific blot, and the stability of biological activity of synthesized polypeptide between batches is greatly increased and errors between batches are greatly induced. Synthesized polypeptide can be kept well in the temperature of four degree for six months without apparent reduction of activity, which is very helpful for the use of reagent box.

The invention discloses a nasal polyp pathological section analysis method and system and a readable storage medium, and the method comprises the steps: collecting the pathological image information of an entity section under a microscope in real time through an image collector, and transmitting the information to a processor; the processor is used for performing effective area detection on all cells on the nasal polyp pathological image, removing an epithelium area, a blood vessel area and a gland area in the current visual field, and then counting the number of plasma cells, neutrophils, eosinophils and lymphocytes in the current visual field; counting the sum of the four types of inflammatory cells as the total number of inflammatory cells in the current view; and then calculating the percentage of plasma cells, neutrophil cells, eosinophil cells and lymphocytes, finally judging the nasal polyp inflammation type of the current slice patient according to the number and proportion of the four types of inflammatory cells under the random 10 high-power lens visual fields and hot spot visual fields in the current slide, and finally storing the statistical result. The invention is helpful for individualized diagnosis and treatment and prognosis improvement of nasosinusitis patients.

The invention discloses an application of kaempferol in medicines for inhibiting rejection reaction of a receptor on organ transplantation. The invention discloses pharmacological effects of kaempferol in inhibiting rejection reaction of organ transplantation in the body of a receptor for the first time; and moreover, optimal effects can be achieved when kaempferol is combined with low-dose cyclosporin for a short time, and long-term survival of an islet graft in the receptor can be induced, so that various side effects caused by using full-dose cyclosporin for a long time can be avoided, the economic burden of patients brought by long-term anti-rejection treatment can also be lightened, and the long-term high-quality life of organ transplantation patients can be further facilitated. The invention also discovers an immune adjustment action mechanism of kaempferol, namely that kaempferol can be used for significantly increasing the number of regulatory T lymphocytes in the body of the receptor and reducing the gene expression of an islet graft IL-6 in the body of the receptor, so that the effects of resisting inflammation and resisting rejection reaction can be achieved.

The invention relates to the technical field of cell culture, and in particular to a cell separation culture solution and a T cell separation culture method. The cell separation culture solution includes a culture solution A, a culture solution B, and a culture solution C, and the three culture solutions cooperate in synergy and work together, and are conducive to stimulating transformation of monocytes to CIK cells. According to the cell separation culture solution and the T cell separation culture method, by adding neutrophil separation fluid to blood cells, content of neutrophils in the cells is reduced; after mononuclear cells are extracted by using the neutrophil separation liquid, most of red blood cells in mononuclear cell layer are removed by using a red blood cell lysis liquid, content of the red blood cells in the mononuclear cells is obviously reduced, and number of lymphocytes is increased; after the final culture, effective cell proportion in the cells is increased, and tumoricidal activity of cell population is increased.

The invention relates to use of compound pachymic acid in the pharmaceutical field. The product has the functions of decreasing the rejection reaction of a receptor to a graft after a transplant operation, protecting the stability of haemolymph cell mitochondrial membrane potential at the periphery of the receptor, reducing the apoptosis percentage of haemolymph cells at the periphery, stabilizing the quantity percentage of CD8 + lymph cells at the periphery, and reducing the apoptosis percentage of the haemolymph cells at the periphery and regulating the quantity percentage of CD4 + and CD8 + lymph cells under nontransplant conditions and so on, is used to reduce graft rejection reactions as well as regulate and stabilize the quantity, the proportion and the function of the lymph cells of organisms during the transplant of organs, tissues and cells of animals and human beings and so on.

The invention belongs to the technical field of freshwater fish culture and especially relates to a feed for promoting intestinal development of juvenile black carp. The feed is prepared from the following raw materials: peanut meal, oat, corn, fresh pine needle, fresh alfalfa, fresh pumpkin vines and leaves, tomato, olive oil, fish meal, Chinese toon juice and myricetin. The feed is easy to digest and absorb, has high absorption conversion rate, and can be used to effectively increase villus height, goblet cell count and lymphocyte count of intestinal tract of juvenile black carp, reduce crypt depth of the intestinal tract, effectively promote rapid growth and perfection of intestinal tract of juvenile black carp, increase nutrient absorption area of the intestinal tract, raise enterocytematuration rate, enhance secretion absorption function, effectively improve the capability of absorbing nutrients, promote intestinal tract's capability of secreting mucoprotein, trefoil peptide andother substances, effectively protect the intestinal mucosa and promote intestinal health. Then, absorption utilization rate of nutrients is effectively increased so as to promote rapid and vigorous growth of juvenile black carp.

A composition for culturing the spleen lymphocytes of laboratory rats comprises an RPMI-1640 culture medium, and concanavalin A, phytohemagglutinin, beta-mercaptoethanol, mannitol, sodium azide, dimethyl sulfoxide and the like are further added into the RPMI-1640 culture medium. Test results prove that the composition can achieve the purpose of maintaining in-vitro long-term proliferation of rat lymphocytes, the number of lymphocytes required by fundamental researches can be achieved through proper proliferation, and the ratio of T cells to B cells is kept unchanged.

The invention provides a medicinal preparation for improving immunity. The medicinal preparation comprises the following raw materials: ginsenoside Rg1 extracted from pseudo-ginseng monomers, whereinthe total ginsenoside Rg1 is ginsenoside. The pharmaceutical preparation provided by the invention has a treatment effect on people with poor immune reconstruction, and can increase the number of CD4+T lymphocytes. Therefore, the preparation can improve and regulate the immunity of a human body, reduce diseases, reduce hyperlipidemia and reduce cardiovascular diseases, can be used as an AIDSadjuvant drug, and can also be used for health care and disease prevention of common people as well as treatment and immunoregulation of people with hyperlipidemia.

A method of diagnosing multiple sclerosis and other demyelinating diseases or predicting a predisposition to multiple sclerosis and other demyelinating diseases. The method utilizes detection of increased amounts of memory lymphocytes reacting to MS antigens, proinflammatory cytokines, and antibodies against MS antigens.

The invention discloses application of a hepcidin antagonist LDN193189 and derivatives thereof to drug preparation, belongs to the technical field of medicine and provides new application of the hepcidin antagonist LDN193189 and the derivatives thereof to preparation of drugs for treating bone marrow form acute radiation sickness and radiation-induced hemocytopenia. The LDN193189 and derivatives thereof are adopted for intraperitoneal injection of mice irradiated by the lethal dose so that the survival rate of the irradiated mice can be increased to 40% (120 days), and the numbers of peripheral blood leukocytes and lymphocytes are obviously increased; the number of bone marrow granulocyte and megakaryocytic hematopoietic progenitor cells is obviously recovered; the survival rate of spleniclymphocytes is obviously recovered; and the number of the splenic lymphocytes is obviously increased (HE splices), and the LDN193189 and the derivatives thereof have good effects on treatment of bonemarrow form acute radiation sickness and radiation-induced hemocytopenia.

The present disclosure provides methods for measuring the number of CD4+ OX40+ Foxp3+ lymphocytes in a sample containing cancer cells and lymphocytes obtained from a subject by labeling lymphocytes that show CD4 expression in the sample, then labeling lymphocytes that show OX40 expression in the sample, then labeling lymphocytes that show Foxp3 expression in the sample, then measuring the number of CD4+ OX40+ Foxp3+ lymphocytes in the sample. Further provided are methods for determining the prognosis of a subject, predicting responsiveness of a subject having cancer to an OX40 agonist treatment, and methods for treating or delaying progression of cancer based on the number of CD4+ OX40+ Foxp3+ lymphocytes in a sample.

The invention relates to a specific lymphocyte content analysis method and device based on TCR / BCR high-throughput sequencing, and belongs to the technical field of gene detection. The analysis methodcomprises the following steps of performing high-throughput sequencing: extracting genome DNA of a biological sample to be detected, adding a predetermined amount of artificially synthesized TCR / BCRcoding gene internal standard sequences, and carrying out high-throughput sequencing; and performing data analysis: obtaining high-throughput sequencing data and TCR / BCR gene sequences obtained by analysis, calculating the amplification multiple according to the input internal standard sequences, comparing the TCR / BCR gene sequences of the sample to be detected with antigen receptor gene sequencesA of target lymphocytes, obtaining the number of original antigen receptor gene sequences consistent with the antigen receptor gene sequences A of the target lymphocyte, the number of sequences withsequencing errors, the number of sequences subjected to secondary rearrangement and the number of mutated sequences, obtaining the total number of target lymph sequences, and calculating the number ofthe contained target lymphocytes according to the total number of the target lymph sequences and the amplification multiple, so that the content of each T / B cell can be accurately determined.

The invention relates to efficient composite probiotics for pets and a method. The efficient composite probiotics are formed by the following components in parts by weight: 11%-25.3% of bifidobacterium animalis, 5%-13.1% of bacillus subtilis, 6%-9.5% of bacillus licheniformis, 7%-12.4% of streptococcus thermophilus, 4.1%-10% of lactobacillus plantarum, 3.5%-12.1% of bacillus coagulans and the balance of protective additive. The efficient composite probiotics are good in bioactivity, and compared with traditional like products,can greatly improve the biological blood oxygen delivery amount, increase the number of leukocytes and lymphocytes, increase the content of in-vivo alkaline phosphatase, increase the content of in-vivo IgG and IgA, effectively improve the balance of intestinal biological colonies and effectively inhibit harmful flora so as to achieve the effects of improving the immunity of organisms, promoting skeletal development, effectively improving the digestion and absorption capability of intestinal tracts on nutrient substances and reducing the damage of harmful florae on intestinal mucosa.

The invention provides a Chinese patent medicine for treating spleen deficiency syndrome postoperative fatigue syndrome and a preparation method thereof. Every 100g of the Chinese patent medicine is prepared from 20-50g of radix aucklandiae,10-30g of fructus amomi, 20-50g of atractylodes macrocephala koidz, 30-70g of prepared fingered citron, 20-50g of steamed pericarpium citri reticulatae, 20-50gof cooked codonopsis pilosula, 20-50g of poria cocos, 10-30g of honey-fried licorice root, 10-30g of ginger processed pinellia, 20-50g of radix salviae miltiorrhizae, 12-35g of sun-dried ginseng, 30-70g of coix seeds, 30-70g of caulis spatholobi, 30-70g of Chinese dates, 20-50g of capillary wormwood, 20-50g of endothelium corneum gigeriae galli and 20-50g of radix dipsaci. Clinical experiments prove that when the Chinese patent medicine is used for treating the spleen-deficiency syndrome postoperative fatigue syndrome, the number of T lymphocytes of a patient can be effectively increased, thepostoperative fatigue degree and the spleen-deficiency syndrome of the patient are improved, and the overall treatment effect on the disease is improved. Particularly, compared with a decoction usingthe same prescription, the Chinese patent medicine is better in curative effect due to the fact that medicinal components in medicinal materials can be better extracted and retained by means of the preparation method.

The invention relates to an application of a compound pachymic acid in the field of pharmacy. With the product, rejection reaction against transplant after transplant operation can be reduced, peripheral blood lymphocyte mitochondrial membrane potential stability can be protected; peripheral blood lymphocyte apoptosis percentage can be reduced, peripheral blood CD8+ lymphocyte number percentage can be stabilized, and so on. Under a non-transplant condition, peripheral blood lymphocyte percentage can be reduced, CD4+ and CD8+ lymphocyte number percentage can be regulated, and so on. When the compound is used for treating, transplant rejection reactions of transplantations of animal and human organ, tissue, cell, and the like can be reduced; and body lymphocyte number, proportion, and function can be regulated and stabilized.

The invention provides a kit and a method for evaluating the curative effect of mesenchymal stem cells on cerebral infarction, and belongs to the technical field of medicines. The kit comprises a reagent for detecting the number of astrocytes, microglia, mononuclear cells, neutrophils and lymphocytes in a cerebral cortex infarction region and a reagent for detecting the expression quantity of TNF-alpha in the cells. The method comprises the following steps: respectively detecting the numbers of astrocytes, monocytes and lymphocytes and the expression quantity of TNF-alpha in a cerebral cortex infarction region before and after the mesenchymal stem cells are transplanted, and if the numbers of the astrocytes, the monocytes and the lymphocytes are lower than those before the mesenchymal stem cells are transplanted, and the expression quantity of TNF-alpha in astrocytes, mononuclear cells and lymphocytes is lower than that before transplantation, determining that the curative effect is good. The kit and the method can efficiently and accurately evaluate the curative effect of the mesenchymal stem cells on cerebral infarction.