The invention belongs to the field of immunological detection and particularly relates to a method for evaluating the blocking activity of an
immune checkpoint molecular
blocking antibody by magneticbeads. Magnetic beads with
solid-phase anti-tag molecular antibodies and
immune checkpoint molecular
protein expressing
label molecules (
label I) subjected to
solid-phase on the surfaces of the magnetic beads are used; the magnetic beads can be specifically bound with a ligand of an
immune checkpoint molecule with another
label molecule (label II), and then a label II specific binding
antibody labeled by a biologic substance (fluorescent or visible light) is used for quantitatively detecting the immune checkpoint molecular
protein / ligand molecular
protein compound on the surface of the
magnetic bead. When the
blocking antibody of the immune checkpoint molecule, the magnetic beads with the immune checkpoint molecule protein in the
solid phase and the ligand protein coexist, the blocking activity of the
antibody is inversely proportional to the final
luminous intensity of the magnetic beads, so that quantitative analysis on the blocking activity of the
antibody is realized through lightintensity detection. The
magnetic bead method is simple to operate, quick in reaction, economical and time-saving.