35 results about "Growth kinetic" patented technology

This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.

Genotype 7a has been identified recently, thus not much is known about the biology of this new, major HCV genotype. The present inventors developed hepatitis C virus 7a / 2a intergenotypic recombinants in which the JFH1 structural genes (Core, E1 and E2), p7 and the complete NS2 were replaced by the corresponding genes of the genotype 7a strain QC69 and characterized them in Huh7.5 cells. Sequence analysis of 7a / JFH1 recombinants recovered after viral passage in Huh7.5 cells following 4 independent transfection experiments revealed adaptive mutations in Core, E2, NS2, NS5A and NS5B. In reverse genetic studies the importance of these mutations for improved growth kinetics was shown. Adapted 7a / JFH1 viruses showed growth kinetics, infectivity and RNA titers comparable to a previously developed 3a / JFH1 reference virus. Conclusion: The developed 7a / JFH1 viruses provide a robust in vitro tool for research in HCV genotype 7, including vaccine studies and functional analyses.

A method of sputter depositing dielectric thin films may comprise: providing a substrate on a substrate pedestal in a process chamber, the substrate being positioned facing a sputter target; simultaneously applying a first RF frequency from a first power supply and a second RF frequency from a second power supply to the sputter target; and forming a plasma in the process chamber between the substrate and the sputter target, for sputtering the target. The first RF frequency is less than the second RF frequency, the first RF frequency is chosen to control the ion energy of the plasma and the second RF frequency is chosen to control the ion density of the plasma. The self-bias of surfaces within said process chamber may be selected; and this is enabled by connecting a blocking capacitor between the substrate pedestal and ground.

The invention provides a method for preparing recombination live vector vaccines for diseases of canid and / or feline based on an RNA (ribose nucleic acid) virus rescuing technique, which is characterized in that a recombination virus expression vector capable of expressing main protective antigens of diseases of the canid and / or the feline based on a reverse genetic manipulation system is established. A rabies virus is conjunctly transfected by the expression vector and assistant plasmids to copy a permissive host cell to rescue the recombination virus, so that multivalent live vector vaccines are prepared. The growth curve of the virus presents that the rescued maternal virus has no obvious difference from the growth kinetic of the recombination virus which expresses the protective antigen genes of main disease pathogens of the canid and / or the feline, and a foundation is established for successfully preparing gene recombination live vector vaccines of main diseases of the canid and / or the feline.

The present invention provides a numerical simulation method for improving the quality of fused molten magnesium lumps and relates to the technical field of quality optimization of fused magnesium products. The method comprises: establishing a macro-micro unified model that couples macro-heat transfer, micro-nucleation and growth kinetics; then using PROCAST software to carry out numerical simulation on the temperature field and microstructure in the process of cooling and solidifying the fused magnesium; carrying out visualization processing and result analysis and discussion on the lump overall temperature field change and the grain growth process; and analyzing the degree of undercooling of the influence trend on the microstructure of the fused molten magnesium lumps. According to the method provided by the present invention, mathematical and physical modeling and numerical simulation are carried out on the heat exchange and the microstructure formation process in the process of cooling and solidifying the fused magnesium lump, and through the control of parameters such as heat exchange conditions and the like, the organization formation is understood and controlled on the solidification rule, so that preparation is made for produce the high-grade periclase, the number of experiments is effectively reduced, manpower and material resources are saved, and the periclase product quality in actual production is improved.