The invention discloses a culture medium for multiplying test-tube seedlings based on somatic embryos of a pear tree. The culture medium is prepared from the following raw materials in parts by weight: 14-26 parts of an organosilicone modified starch water emulsion, 10-20 parts of 1 / 2 modified SH, 20-30 parts of sucrose, 20-30 parts of non-ionic polyacrylamide, 5-11 parts of graft modified soybeanprotein, 15-25 parts of alternaria tenuissima protein, 11-19 parts of carbonase, 11-19 parts of sodium p-nitrophenolate, 6-8.4 parts of eugenol, 1.5-6.5 parts of paclobutrazol, 1-3 parts of bacilluscereus, 3-6 parts of a beta-compound detoxification enzyme, 3-5 parts of bacillus natto, 3-5 parts of 6-benzyl aminopurine, 0.5-1.0 part of naphthylacetic acid and 3-6 parts of 2, 4-D. Through optimization of the components of the culture medium, the survival rate of transplanted plants is greatly increased, the incidence of diseases of the seedlings is reduced and the seedlings grow healthily andstrongly.