58 results about "Cell wall degradation" patented technology

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

A nectarine juice drink and methods of producing a nectarine juice drink are described. The method involves (a) providing nectarines; (b) treating the nectarines with heated, moist air or heated water to produce heat treated nectarines; (c) pressing the heat treated nectarines through one or more filters to produce a nectarine juice solution and (d) combining the nectarine juice solution with one or more plant cell wall degrading agents to produce a nectarine juice drink.

A process for recovering corn germ and corn coarse fiber from corn in a dry grind process, involving soaking corn kernels in water to produce soaked corn kernels, grinding the soaked corn kernels to produce a ground corn slurry, and incubating the ground corn slurry with at least one enzyme(amylase(s), protease(s), cell wall degrading enzyme(s), or mixtures thereof, and optionally other enzyme(s)) to increase the specific gravity of the slurry to about 10-about 16 Baume so that the corn germ and corn coarse fiber floats to the top of the slurry, recovering the corn germ and the corn coarse fiber, and optionally producing ethanol from the slurry no longer containing the corn germ and corn coarse fiber. The process does not involve the addition of starch, a salt, a sugar syrup, or mixtures thereof to the slurry.

The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose. These systems have a number of applications.

The invention discloses a high plant cell wall degradation activity rumen fungi, and applications thereof in feed ensiling, and belongs to the technical field of microorganism and feed processing. According to a preparation method, Hungate anaerobic roll-tube technique is adopted to obtain an anaerobic fungi with cell wall degradation enzyme activity via separating and selecting from the rumens of Xinong Saanen dairy goats, finally obtained Piromyces sp.CN6 is capable of producing xylanase and acetylesterase with relatively high activity in a wide temperature and pH range, and excellent thermal stability and acidic and alkaline stability, the preservation number is CGMCC No.14449. The bacteria strain is capable of improving fermentation quality of silage, increasing crude fiber degradation rate, and can be applied in practical production as a silage additive.

The invention discloses a soil treatment agent for preventing and treating the root-knot nematode disease and a preparation method and an application method thereof. The soil treatment agent is prepared from a nematode killing main agent and a nematode killing synergist; the nematode killing main agent is prepared from a nematode killing organic raw material and a decomposition promoting accessory; when the nematode killing organic raw material is a solid organic raw material, the nematode killing organic raw material is prepared from at least one of crop straws and sugar refinery offcut; whenthe nematode killing organic raw material is a liquid organic raw material, the nematode killing organic raw material is prepared from at least one of alcohol fermentation liquor and molasses; the decomposition promoting accessory is prepared from at least one of organic catalytic material and a special microorganism. The soil treatment agent disclosed by the invention can secrete to generate a great amount of nematode killing substances, such as an cell wall degrading enzyme, acetic acid and the like, in the indigenous microorganism anaerobic decomposition process, and a rapidly created anaerobic environment can also kill nematodes, so that the number of nematodes in soil with a high root-knot nematode disease incidence rate and a proportion of the category in a nematode community are reduced, and a root-knot nematode disease incidence rate of succeeding crops is reduced.

The invention relates to enzymes, compositions, and methods for efficient hydrolysis of arabinans present in plant biomass. More specifically, the invention relates to arabinases and compositions comprising arabinases to improve cell wall degradation to efficiently use plant biomass for bio-energy production. The invention also relates to a method for preparing a prebiotic. The prebiotic may contain branched arabinan oligomers comprise α-(1,5)-linked arabinan backbone, and single substituted α-(1,3)-linked arabinose monomers attached to the backbone, or double substituted α-(1,2,3,5)-linked arabinose monomers attached to the backbone, or both. The invention also relates to a method for preparing fruit juice or wine. The invention also relates to a method for saccharification of a plant biomass. The invention also relates to a recombinant micro-organism genetically modified to express the enzymes of the present invention and optionally additional enzymes to achieve the disclosed methods.

The invention discloses a method for extracting and separating cyclocarya paliurus flavonoids, wherein the method comprises the following steps: a, washing cyclocarya paliurus with clear water, dryingat the temperature of 40-50 DEG C, crushing the sample by a pulverizer and allowing to pass through a 40 mesh sieve; b, carrying out acidolysis; c, carrying out microbial fermentation degradation; d,carrying out compound enzymatic hydrolysis; e, carrying out acetone extraction; f, carrying out purification of crude flavonoids: purifying flavonoids with activated carbon. In the extraction processof the cyclocarya paliurus flavonoids, firstly, cell walls of cyclocarya paliurus are degraded by the preliminary microbial fermentation method, and some impurities, such as polysaccharides, are removed by microbial action; then the flavonoids are fully released by enzymatic hydrolysis. The method has no pollutant discharge in the whole technological process, is suitable for large-scale production, is beneficial to the development and utilization of cyclocarya paliurus resources, has the advantages of simple steps, low cost, strong operability and high output, and is prone to industrialized production.