115 results about "Enzyme variant" patented technology

The present disclosure provides improved genome editing compositions and methods for editing a CBLB gene. The disclosure further provides genome edited cells for the prevention, treatment, or amelioration of at least one symptom of, a cancer, an infectious disease, an autoimmune disease, an inflammatory disease, or an immunodeficiency.

The present disclosure provides improved genome editing compositions and methods for editing a CBLB gene. The disclosure further provides genome edited cells for the prevention, treatment, or amelioration of at least one symptom of, a cancer, an infectious disease, an autoimmune disease, an inflammatory disease, or an immunodeficiency.

The present invention relates to detergent compositions comprising lipase variants. The present invention also relates to lipase variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

A number of human beta-glucuronidase variants having higher enzymatic activity at physiological pH as compared with wild-type beta-glucuronidase and uses thereof in prodrug therapy. Also disclosed herein is a method for identifying enzyme variants having elevated enzymatic activity using a mammalian surface display system.

The present invention relates to detergent compositions comprising a detergent ingredient and a lipase variant with reduced potential for odor generation obtained by introducing mutations in one or more regions identified in a parent lipase.

Human cystathionine β-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine β-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine β-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.

The present invention provides methods of designing and generating polypeptide variants that have altered properties compared to a parent polypeptide. The present invention further provides a computer program product for carrying out the design of a variant polypeptide. The present invention further provides nucleic acids encoding enzyme variants, as well as vectors and host cells comprising the nucleic acids. The present invention further provides variant enzymes; methods of producing the variant enzymes; and methods of producing compounds using the enzymes.

The present invention provides methods for engineering proteins to optimize their performance under certain environmental conditions of interest. In some embodiments, the present invention provides methods for engineering enzymes to optimize their catalytic activity under particular environmental conditions. In some preferred embodiments, the present invention provides methods for engineering enzymes to optimize their catalytic activity and / or stability under adverse environmental conditions. In some preferred embodiments, the present invention provides methods for engineering enzymes to optimize their storage stability, particularly under adverse environmental conditions. In some preferred embodiments, the present invention provides methods for altering the net surface charge and / or surface charge distribution of enzymes (e.g., metalloproteases) to obtain enzyme variants that demonstrate improved performance and / or stability in detergent formulations as compared to the starting or parent enzyme.

The present invention related to lipase variants having lipase activity and having between 60% to less than 100% sequence identity to a parent lipase or a fragment thereof, wherein the variant comprises one or more substitutions selected from H198A / D / E / F / G / I / L / N / Q / S / T / V / Y, F7H / K / R, F51A / I / L / V / Y, T143A / G / S / V, A150G / V, N200H / K / Q / R, I202G / L / V, S224C / F / H / I / L / P / Y, L227D / E / K / R, V228P, P229H / K / R, V230H / K / L / R, I255A / G / N / P / S / T / V / Y, P256A / K / N / Q / R / S / T / W, A257F / H / I / L / V / Y, L259F / Y, and W260D / E / F / H / I / L / N / Q / S / T / Y using SEQ ID NO:10 for position numbering or selected from H198A / D / E / F / G / I / L / N / Q / S / T / V / Y, F7H / K / R, F51A / I / L / V / Y, T143A / G / S / V, A150G / V, N200H / K / Q / R, I202G / L / V, S224C / F / H / I / L / P / Y, L227D / E / K / R, V228P, P229H / K / R, V230H / K / L / R, I255A / G / N / P / S / T / V / Y, T256A / K / N / Q / R / S / P / W, A257F / H / I / L / V / Y, L259F / Y, and W260D / E / F / H / I / L / N / Q / S / T / Y using SEQ ID NO:2 for position numbering. Further the invention relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; to compositions comprising the variants as well as methods of producing and using the variants.

The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.

The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and / or an increased activity towards amylopectin and / or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and / or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.