43 results about "Cell tissue culture" patented technology

The invention discloses a bio-macromolecular hydrogel prepared by enzyme catalysis and ion cross-linking, and a preparation method of the bio-macromolecular hydrogel. The bio-macromolecular hydrogel comprises a protein or polypeptide bio-macromolecular network formed by enzyme-catalyzed cross-linking of protein or polypeptide or amino acid residue-containing molecule, accounting for 1-99 wt% of total dry mass of the hydrogel; and a polysaccharide bio-macromolecular network formed by bivalent ion cross-linking of polysaccharide macromolecule, accounting for 1-99 wt% of total dry mass of the hydrogel. The above two networks are inter-penetrated without chemical bonding. The hydrogel has the advantages of excellent mechanical properties, no use of chemical cross-linking agent, simple and effective preparation method, good bio-compatibility and mechanical strength, and capability of steam sterilization; may be in the forms of wet or dry film, porous sponge, tube and particles; and can be used in cell / tissue culture, and used as tissue repair material, tissue engineered scaffold or drug release carrier.

The present invention relates to a three dimensional construct formed from non-biodegradable and non-cytotoxic polymers that provide an internal and external space for living cells to attach, proliferate and differentiate. The construct is composed of polymer struts and / or fibers which are joined together in a designed 3 dimensional pattern. The 3 dimensional cell culture construct (cell culture insert) is intended to be used together with cell / tissue culture plate, tissue culture flask, bioreactor and the like under normal cell culture conditions. The invention further provides methods of making the 3 dimensional cell culture construct. Finally, the invention provides kits comprising one or more 3 dimensional porous cell culture construct in a package together with other cell culture supplies, such as tissue culture plate and flasks.

The invention relates to a method for cultivating saussurea involucrate cell, wherein it comprises that using MS basic culture medium wit some NNA (fruitone), 6-BA (6-aminopurine) as the culture medium of callus; inducing the seed of saussurea involucrate into plant; using said plant as expalnt to be differented at special condition, to induce callus; subculturing the callus periodically, to increase the callus; using part of callus to function the seed and collecting the left to be dried as the saussurea involucrate culture; collecting said culture to be dried as the cell culture. The invention can improve the content of effective components.

The invention relates to a device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells, in particular to a device and method for noninvasive continuous on-line or off-line monitoring of quantity or concentration of dynamic cells in adherent cell cultures (usually as stem cell cultures or other therapeutic cell cultures) or cell and tissue cultures. The device comprises: a cell culture medium supply system, one / one type of or multiple / multiple types of upstream biomedical indicator detection head(s) or connector(s), a cell culture , one / one type of or multiple / multiple types of downstream biomedical indicator detection head(s) or connector(s), one or multiple speed controllable sterile driving unit(s) of fluid, a waste or harvested liquor system, liquid conveying pipeline systems that are driven and controlled in speed by the speed controllable sterile driving unit(s) of fluid for connecting the above components in order. And the upstream and downstream biomedical indicator detection head(s) or connector(s) are connected to a monitoring and controlling system and / or a computer so as to obtain, process and monitor data, and furthermore provide feedbacks for the whole cell culture system through a signal feedback system for realizing control.

The invention discloses a micro-flow control chip for cell tissue culture and real-time monitoring and a use method thereof. The chip comprises a glass substrate layer and a PDMS micro-flow channel layer positioned on the glass substrate layer; the glass substrate layer comprises a glass substrate and a plurality of pairs of microelectrodes arranged on the glass substrate layer; the PDMS micro-flow channel layer comprises a plurality of independent micro-fluidic channels; the microelectrodes on the glass substrate are corresponding to the micro-flow channels on the PDMS micro-flow channel layer one by one; the microelectrodes are electrically connected with an external circuit. The use method comprises the steps of cell capture, cell tissue culture, electrical impedance spectroscopy detection and tissue release. The chip disclosed by the invention is processed by a transparent substrate, microscopic imaging can be taken as a supplementary means of the electrical impedance spectroscopydetection, and the dynamic growth and the physiological behavior of cell tissues and other biological processes are subjected to observation and in-situ analysis. The micro-flow control chip can be used in the fields of biological research, drug screening and the like of cells or tissues.

A process for producing a collagen sponge and process for producing an artificial skin, in which a final collagen sponge and artificial skin can be obtained with high yield without occurrence of defectives through simplified steps; and a cell tissue culture substrate and artificial skin utilizing the collagen sponge produced by the above collagen sponge production process. There is provided a process for producing a collagen sponge, comprising using a dilute collagen solution having its pH adjusted with acetic acid.

A cell / tissue culture apparatus is suitable for a culture of a cultivated object of a cell or tissue adapted for a prescribed portion of a living body of a breast and so on, a culture chamber (18, a culture vessel 10) which accommodates a cell or tissue to be cultivated and also makes a culture fluid (34) circulate is formed by a flexible material (flexible films 12, 14), and the culture chamber is immersed in a fluid (water 8). A pressure or an oscillation is given to the culture chamber installed in the fluid, and a physical stimulation due to the pressure from the fluid or the oscillation of the fluid is given to a cell or tissue under a culture.

The invention discloses a cell tissue culture system capable of accurately controlling air pressure. The cell tissue culture system comprises a culture room, a high pressure air source, an air flow meter, an air inlet pipeline, an air inlet switch, an air outlet pipeline, a pressure sensor, a decompression device, an exhaust valve and a control device, wherein the high pressure air source is communicated with an air inlet of the culture room through the decompression device, the air flow meter and the air inlet pipeline; the air inlet switch is arranged on the air inlet pipeline; an air outlet of the culture room is connected with the air outlet pipeline; the air outlet pipeline is communicated with the outside through the exhaust valve; the exhaust valve is used for controlling air in the culture room to be quantitatively updated; the air flow meter is used for measuring air intake flow in real time; the pressure sensor is used for sensing the air pressure in the culture room and on the air inlet pipeline and the air outlet pipeline; and the control device is used for controlling the air inlet switch to be turned on or off according to a signal from the pressure sensor. According to the cell tissue culture system, the air pressure in the culture room is stabilized to be in the preset level for a long time, and the air in the culture room is constantly updated to guarantee the accuracy of scientific research.

The invention discloses a method for improving the characteristics of wild broussonetia papyrifera through germ cell culture. The method comprises the steps of (1) taking wild broussonetia papyrifera as female parent; (2) selecting and slicing germ; (3) sterilizing the germ; (4) inducing callus; (5) promoting differentiation of the callus into seedling to obtain a complete plant; and (6) managing after differentiating into seedling. The method disclosed by the invention, on the basis of germ cell tissue culture technology, enhances fast growth performance of the wild broussonetia papyrifera, lengthens wood fiber and increases the content of plant protein in tender shoots and leaves, so that the wild broussonetia papyrifera can grow at ultra-fast speed and have long wood fiber, and the tender shoots and leaves are rich in nutrition.

It is an object of the present invention to provide a method for producing a collagen sponge and a method for producing an artificial skin which can give a high yield through simplified steps, no inferior goods being produced in the finally-obtained collagen sponges and artificial skins; and an artificial skin and a substrate for tissue engineering which respectively comprise a collagen sponge produced by the method for producing a collagen sponge. The present invention is a method for producing a collagen sponge, wherein a diluted collagen solution having a pH adjusted by use of acetic acid is used.

The invention relates to a method for the large-scale production of jatropha curcas plants by cell-tissue culture, belonging to the culture technology of plant seedlings. The method comprises the following steps: (1) cell suspension culture: grinding and inoculating jatropha curcas tissue and adding a cell suspension culture substrate: 0.2-0.5 mg / L of MS+BA, 1-6 mg / L of NAA, 0.5-1.5 mg / L of LH and 19-22 g / L of sucrose; (2) cell growth measurement; (3) clumpy bud multiplication culture: selecting cells growing into a resting stage to access a clumpy bud multiplication culture substrate: 0.1-1 mg / L of MS+6-BA, 0.1-1.5 mg / L and 20-30 g / L of sucrose; (4) rooting culture: selecting well-growing clumpy buds to access a rooting culture substrate: 0.1-1 mg / L of MS+NAA; and (5) bottling culture. The invention organically combines the cell culture and the tissue culture of jatropha curcas and has the advantages of short period and low cost, provides a high-efficiency path for manually culturing the jatropha curcas, producing virus-free seedlings and culturing fine varieties and has a favorable prospect for developing and utilizing the jatropha curcas.

Tissue culture medium such as porous frameworks and even open surface multidirectional porous frameworks may be used to provide uniform distribution of nourishment solutions, uniform interstitial voids as well as undistorted transport fields which may facilitate high volume yields of finished plants from cells, such as explants in a tissue culturing process. Further embodiments may include automating a tissue culturing process to reduce labor costs and increase uniformity of finished plants through tissue culture processes.

In order to detect the mis-operation of cells or tissues in a culturing step and other working steps to prevent erroneous use in treatment of cultured cell and tissues besides those of the cell donor, a genetic information, held by a cell or tissue, is used as a cell tissue information, which is inseparable from the cell tissue itself and enables individual identification of the cell donor, that is, identification of the origin of the cell or tissue subject to a culture process, and the cell tissue information before and after culturing are compared and collated.

The invention discloses a method for separating extracellular microcapsules from cells, tissue culture supernatant or body fluid, a specialized separating reagent, a kit and application. The method comprises the steps of alienating extracellular microcapsules in cells, tissue culture supernatant or body fluid from a water solution by virtue of a polyethylene glycol (PEG) solution, and concentrating and collecting the settle extracellular microcapsules by virtue of a low-speed centrifugation method. By virtue of the method, 1000mL or above specimens can be processed in each time; compared with ultracentrifugation and chromatography methods, the method has the advantages that the operation is simple, the elapsed time is short, large-sized instruments and equipment are not required, and the repeatability is good; and the problem that the extracellular microcapsules are difficult to separate is solved, and the scientific research and the clinical application of the extracellular microcapsules are promoted.

The invention relates to an anti-browning culture method of Haworthisa.spp cells. The method is characterized in that the method comprises the following steps: 1, inoculating the Haworthisa.spp cell culture into a sterile triangular flask, adding a culture solution into the triangular flask, putting the sterile triangular flask on a shaking table for proliferation suspension culture, taking the filtrate of the Haworthisa.spp cell culture obtained in the previous step, adding the culture solution, inoculating the material into the sterile triangular flask, and putting the sterile triangular flask on the shaking table for anti-browning suspension culture. The method directly takes the callus cells of the Haworthisa.spp as explants, firstly carries out suspension culture and then carries outsolid culture; meanwhile, the Himalaya glacier water is used for culturing the Haworthisa.spp cell tissue, and the glacier water can be used as an anti-browning agent, so that the browning phenomenonin the Haworthisa.spp cell tissue culture can be effectively prevented and solved, and can also be used as an additive to be added into a culture medium to promote proliferation of plant cell tissues,and scientific reference is provided for tissue culture and industrialization of the Haworthisa.spp cells.

The invention provides a device and method for in-vitro 3D culture of cells and tissue. The device includes a cover layer and a cell-tissue culture chamber layer, wherein the cover layer includes a liquid inlet hole, a liquid outlet hole, Luer joints are arranged on upper parts and lower parts of the liquid inlet hole and the liquid outlet hole, and the Luer joints can be externally connected to PE tubes; and the cell-tissue culture chamber layer includes multiple cell-tissue organ culture tanks, and buckle arms are arranged on both sides of each culture tank. When the device is applied to cell culture, multiple cell-tissue culture experiments can be performed in vitro at the same time, and the culture state of cells and tissue can be detected and monitored conveniently and quickly throughthe microfluidic design.

The invention relates to a health product and a preparation method thereof. The preparation method includes the steps A, inoculating Ganoderma sinensis to culture solution, and performing shake culture on a shaker to obtain fungus solution; B, inoculating the fungus solution obtained in the step A to liquid medium containing barley malt and soybean, and performing aerobic or anaerobic culture to obtain fermented Ganoderma sinensis mycelia and Ganoderma sinensis fermentation broth; C, subjecting the Ganoderma sinensis mycelia obtained in the step B to cell tissue culture breakage, and extracting with water to obtain Ganoderma sinensis extract; and D, mixing the Ganoderma sinensis fermentation broth obtained in the step B and the Ganoderma sinensis extract obtained in the step C, and diluting or concentrating obtained mixed liquor to obtain the Ganoderma sinensis health product. By the industrial submerged fermentation technology, the Ganoderma sinensis culture can be propagated industrially and massively in the liquid medium. Therefore, the process is simple, and the cost is low.

The invention discloses a preparation method and application conditioned medium for deer periosteum, and belongs to technical field of cell tissue culture. The method provided by the invention is as below: inserting deer periosteum tissue slice in an insert dish, adding medium into the insert dish, collecting culture solution after culture, filtering to obtain conditioned medium. The insert dish is composed of an upper chamber and a lower chamber, and is placed into a porous culture plate. The method provided by the invention can make the deer periosteum tissue secrete active substances under in vitro culture conditions, so as to collect and analyze the secreted active substances. The method solves the problems that in vitro culture cannot provide three-dimensional cultivation environment for the tissue, carbon dioxide concentration is difficult to control in the cultivation process of and conditioned medium can not be collected momentarily. At the same time, the method provided by the invention effectively improves the survival rate of cultured tissue, shortens the culture time of the tissue, and can collect conditioned medium at any time.

The invention belongs to the field of biomedical cell tissue culture and experiments, and discloses a stimulation culture device and a stimulation culture method for applying two stresses including atraction force and a pressure to cells, controllable time sequences are realized for a culture chamber to respectively realize pressurization and pressure extraction, and the same-frequency stretchingand retraction of an elastic culture cavity are matched to realize the joint stimulation of any pressure and tensile strain on the cells in a controllable range, and a simple and effective device anda technical scheme for forming strain and pressure on the cells by the tensile force and carrying out linkage adjustable stimulation culture on the cells at the same time are provided.

The invention discloses a PEG-grafted polysulfone or polyethersulfone hollow fiber membrane and its preparation method and application. : After mixing in a ratio of 3 to 9, it is dissolved in a solvent such as N-methylpyrrolidone with a weight ratio of 15 to 30%, dissolved under stirring for 10 to 12 hours, and degassed to obtain a spinning stock solution. Two concentric hollow fiber spinnerets are used to extrude the spinning dope through the spinnerets at a speed of 5-20ml / min. The as-spun fibers travel 10-30cm in the environment, and then solidify and form in a water bath at a temperature of 50-80°C, with a fluid linear velocity of 5-40m / min. The hollow fiber membrane has the beneficial effect of anti-platelet, protein and small molecule adsorption, and can be used as a hemodialysis device, or used in bioartificial organs for tissue culture of various human or animal cells, drug metabolism, toxicity and pathology research.

The invention relates to the technical field of cell tissue culture devices, and particularly relates to a negative pressure culture device convenient for collagen extraction. The negative pressure culture device comprises a shell, wherein the shell is connected with a cover plate; a partition plate is arranged in the shell; a sealing ring is arranged below the partition plate; a placement hole is formed in the partition plate; a bearing and a limiting rod are arranged on the bottom surface of the shell; the bearing is connected with a rotary column; and the rotary column is connected with a plugging block, a culture dish and a tray. A culture solution is added into a storage tank and reaches a liquid outlet through a conveying pipe and a hose, an electric push rod pushes the liquid outlet to the upper part of the culture dish, and the culture solution can be added into the culture dish under the condition that the internal space of the device is not opened, so that the device is more convenient and practical; and when the culture dish is taken and stored, only the rotary column needs to be rotated, so that the tray drives the culture dish to move upwards and jacks the plugging block open, the placement hole is always in a plugged state, the interior of the device can be still in a relatively closed state, the internal pressure cannot be greatly changed, and a good culture environment is kept.

A method for increasing the efficiency of homologous recombination-based gene editing in a plant according to an embodiment of the present invention includes optimizing temperature and photoperiod conditions during tissue culture of plant cells, expressing factors required for homology-directed DNA repair (HDR) and factors for increasing the HDR efficiency by using a multiple replicon, or regulating the HDR pathway or non-homologous end joining (NHEJ) pathway.

The invention discloses a microfluidic chip for cell tissue culture and real-time monitoring and a method for using the chip. The chip includes a glass substrate layer and a PDMS microfluidic channel layer on the glass substrate layer. The glass substrate layer includes a glass substrate and a glass substrate. Several pairs of microelectrodes arranged on the glass substrate layer, the PDMS microfluidic channel layer includes several independent microfluidic channels, and the microelectrode pairs on the glass substrate correspond to the microfluidic channels on the PDMS microfluidic channel layer; The microelectrodes are electrically connected to external circuits. The methods of use include cell capture, cell tissue culture, electrical impedance spectroscopy detection, and tissue release. The chip of the present invention is processed with a transparent base material, and the microscopic imaging can be used as a supplementary means for electrical impedance spectroscopy detection to observe and analyze biological processes such as dynamic growth and physiological behavior of cell tissues in situ. It can be used in fields such as biological research of cells or tissues and drug screening.

A method for cultivating and breeding cell tissue of the tissue culture seedling of Dendrobium candidum comprises the steps of mother plant treatment, soaking disinfection, cleaning and cutting, callus induction, callus crushing, shake cultivation, protocorm cultivation, adventitious bud cultivation, plantlet cultivation and sapling cultivation. According to the invention, good characters of the previous generation are stably inherited by the next generation through a method of asexual reproduction, so that the phenomenon of character segregation of the filial generation, brought by sexual reproduction, can be avoided effectively, the characters of the mother plant can be reserved best, and the production of tissue culture seedling has purposiveness and pertinence.

The invention discloses a cell tissue culture system capable of accurately controlling air pressure. The cell tissue culture system comprises a culture room, a high pressure air source, an air flow meter, an air inlet pipeline, an air inlet switch, an air outlet pipeline, a pressure sensor, a decompression device, an exhaust valve and a control device, wherein the high pressure air source is communicated with an air inlet of the culture room through the decompression device, the air flow meter and the air inlet pipeline; the air inlet switch is arranged on the air inlet pipeline; an air outlet of the culture room is connected with the air outlet pipeline; the air outlet pipeline is communicated with the outside through the exhaust valve; the exhaust valve is used for controlling air in the culture room to be quantitatively updated; the air flow meter is used for measuring air intake flow in real time; the pressure sensor is used for sensing the air pressure in the culture room and on the air inlet pipeline and the air outlet pipeline; and the control device is used for controlling the air inlet switch to be turned on or off according to a signal from the pressure sensor. According to the cell tissue culture system, the air pressure in the culture room is stabilized to be in the preset level for a long time, and the air in the culture room is constantly updated to guarantee the accuracy of scientific research.

The invention particularly relates to a biological cell stimulation system based on a pneumatic annular mold, and a control method thereof. The biological cell stimulation system comprises an annular culture mold and air pump equipment; the annular culture mold is arranged in a culture dish and is connected with the air pump equipment through an air guide pipe; the annular culture mold comprises an upper mold, a lower mold and a mold base; the upper mold and the lower mold are in sealed connection, and the lower mold and the mold base are in sealed connection, so that the volume-variable annular culture mold is formed; the mold base is arranged in the culture dish; and a through hole is formed in the upper mold, and the air pump equipment is connected with the lower mold through an air guide pipe via the through hole. The system has no special requirements in cell tissue culture differentiation promotion application, does not need complex treatment on a cell sample, can realize integration of culture and stimulation, effectively reduces the damage and microbiological contamination probability of the cell tissue in the culture and stimulation process, and can be used for research on skeletal muscle tissue engineering, drug screening and life-like robots taking muscle cells as driving units.

The invention relates to the technical field of animal cell tissue culture, in particular to an animal cell tissue culture device and a culture method.The device comprises a culture platform, a connecting column, a culture frame, a cover plate, a shaking mechanism, a bearing disc, a culture dish and a feeding mechanism; a rotating disc drives a vertical moving block to move up and down on an annular adjusting plate in a reciprocating manner, so that the vertical moving block synchronously drives a bearing disc to shake in the process of driving the bearing disc to rotate through a shaking rod, and a nutrient solution in a culture dish is shaken and shaken up; in the feeding mechanism, according to the amount of nutrient solutions needed in all culture dishes, a valve is opened, air is extruded through an injection cylinder, the nutrient solutions are pumped into the culture dishes through cooperation of a liquid distribution pipe and a connecting pipe, a cover plate is not opened in the whole feeding process, and therefore the possibility that infectious microbes enter a cavity is reduced, and the feeding efficiency is improved. And the possibility of animal tissue cell culture polluted by infectious microbes is reduced.

The invention discloses a method for cultivating yew cell tissue culture. Specifically, yew seeds are sterilized, cleaned, soaked, and seed-coated, inoculated on an induction medium, and cultured in the dark. After the seeds germinate , cultivated into yew plants; select well-grown yew plants as explants; inoculate the explants on the medium, and cultivate them in the dark; then transfer them to a culture room with a temperature of 25°C and 28°C for cultivation; select vigorous growth , no browning, and yellow-white callus are inoculated into the subculture medium for periodic subculture; after that, select seeds, inoculate the seeds into the subculture medium, light culture, and collect after 30 to 35 days of culture , Taxus cell tissue culture. The paclitaxel content of the yew cell tissue culture provided by the present invention can reach 15‰-17‰, and the yew cell tissue does not brown during the passaging process, and the content of paclitaxel produced does not decrease, and the temperature and light conditions can still be changed. Steady growth.