Cell tissue culture system capable of accurately controlling air pressure and control method thereof

Abstract

The invention discloses a cell tissue culture system capable of accurately controlling air pressure. The cell tissue culture system comprises a culture room, a high pressure air source, an air flow meter, an air inlet pipeline, an air inlet switch, an air outlet pipeline, a pressure sensor, a decompression device, an exhaust valve and a control device, wherein the high pressure air source is communicated with an air inlet of the culture room through the decompression device, the air flow meter and the air inlet pipeline; the air inlet switch is arranged on the air inlet pipeline; an air outlet of the culture room is connected with the air outlet pipeline; the air outlet pipeline is communicated with the outside through the exhaust valve; the exhaust valve is used for controlling air in the culture room to be quantitatively updated; the air flow meter is used for measuring air intake flow in real time; the pressure sensor is used for sensing the air pressure in the culture room and on the air inlet pipeline and the air outlet pipeline; and the control device is used for controlling the air inlet switch to be turned on or off according to a signal from the pressure sensor. According to the cell tissue culture system, the air pressure in the culture room is stabilized to be in the preset level for a long time, and the air in the culture room is constantly updated to guarantee the accuracy of scientific research.

Description

technical field [0001] The invention relates to a cell tissue culture device capable of precisely controlling gas pressure and a control method thereof. Background technique [0002] Providing a cell culture system that simulates the human body environment is of great significance for medical research. There are many kinds of cells in the human body, such as vascular endothelial cells, retinal ganglion cells, cardiomyocytes, neuron cells, etc. All kinds of cells in the human body live in a certain pressure environment, and the increase of the pressure will have an important impact on the cells. manifested as a pathological state. For example, high blood pressure, high intracranial pressure, high intraocular pressure, etc. In an in vitro setting, an increase in cell hydrostatic pressure can be achieved by increasing the pressure of the cell culture gas. For in vitro cell culture, CO is routinely required 2 The gas acts as a buffer system, the cells are immersed in the cul...

AI Technical Summary

Problems solved by technology

When the pressure of the device increases, the internal and external pressure of the entire culture bottle is unbalanced, which puts forward special requirements for the culture bottle itself, not only the bottle cap with double openings, the bottle body that can withstand the pressure increase, but also the cell attachment, Surface-treated culture interior surfaces, while no culture bottle product currently on the market meets all of these needs

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