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Biological cell stimulation system based on pneumatic annular mold, and control method thereof

A technology of biological cells and ring molds, which is applied in the field of biological cell culture systems, can solve the problems of increasing the complexity of the process and the probability of bacterial contamination, and achieve the effect of reducing damage and bacterial contamination and improving differentiation efficiency

Pending Publication Date: 2021-08-27
SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although similar mechanical stimulation methods can promote cell growth and differentiation, cell tissue culture and stimulation need to be completed on two systems separately, which increases the complexity of the process and the chance of bacterial contamination

Method used

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  • Biological cell stimulation system based on pneumatic annular mold, and control method thereof
  • Biological cell stimulation system based on pneumatic annular mold, and control method thereof
  • Biological cell stimulation system based on pneumatic annular mold, and control method thereof

Examples

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Effect test

Embodiment 1

[0049] 1) Add myoblasts (C2C12), matrigel, fibrinogen, thrombin, and medium biological mixture into the circular culture mold 1 made by PDMS. After the mixture is solidified, add 10 milliliters of cell proliferation medium (89% volume of high-glucose DMEM medium + 10% volume of fetal bovine serum + 1% volume of penicillin-streptomycin mixture) to submerge the biogel in the cell culture mold, and cultivate in a cell culture incubator with 5% carbon dioxide at 37 degrees Celsius ;

[0050] 2) Replace the new cell proliferation medium every day until after 24 hours of culture, suck out the cell proliferation medium in the culture dish, wash the cells with sterile phosphate-buffered saline (PBS) 3 times for 3 minutes each time, and add the cells Differentiation medium (97% volume of high-sugar DMEM medium + 2% volume of horse serum + 1% volume of penicillin-streptomycin mixture), continue to cultivate at 37 degrees Celsius in a cell culture incubator with 5% carbon dioxide;

[00...

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Abstract

The invention particularly relates to a biological cell stimulation system based on a pneumatic annular mold, and a control method thereof. The biological cell stimulation system comprises an annular culture mold and air pump equipment; the annular culture mold is arranged in a culture dish and is connected with the air pump equipment through an air guide pipe; the annular culture mold comprises an upper mold, a lower mold and a mold base; the upper mold and the lower mold are in sealed connection, and the lower mold and the mold base are in sealed connection, so that the volume-variable annular culture mold is formed; the mold base is arranged in the culture dish; and a through hole is formed in the upper mold, and the air pump equipment is connected with the lower mold through an air guide pipe via the through hole. The system has no special requirements in cell tissue culture differentiation promotion application, does not need complex treatment on a cell sample, can realize integration of culture and stimulation, effectively reduces the damage and microbiological contamination probability of the cell tissue in the culture and stimulation process, and can be used for research on skeletal muscle tissue engineering, drug screening and life-like robots taking muscle cells as driving units.

Description

technical field [0001] The present invention relates to a biological cell culture system that can generate any variable cycle and multiple modes of mechanical stimulation, specifically, it is realized by combining the uniform stress of the ring-shaped culture mold and the controllability of arbitrary gas output, maintenance and release of the air pump equipment Mechanical strain stimuli of any frequency, duration, and amplitude. It is mainly used for electrical stimulation of biological cells to promote growth and differentiation, and has important potential applications in the field of life sciences, such as skeletal muscle tissue engineering, drug screening, and research on life-like robots with muscle cells as the driving unit. Background technique [0002] The life-like robot system formed by the organic deep integration of life system and electromechanical system may have the advantages of biology and machine, such as the unique multi-degrees of freedom of life, high en...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M3/00C12M1/00
CPCC12M35/04C12M41/00C12M41/40
Inventor 刘连庆张闯王文学于鹏解勇宝
Owner SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
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