Double-stress stimulation culture device and method thereof

A technology that stimulates culture and double stress, and is applied in the methods of stress stimulating microbial growth, tissue cell/virus culture devices, biochemical cleaning devices, etc. Circulation and other issues to achieve the effect of saving usage, increasing data range, and reducing interference

Pending Publication Date: 2020-10-23
上海天引生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] According to the published invention patent application No. 2017107159633, a cell culture device under the associated action of pressure and unidirectional strain and its method, the pressure and unidirectional strain described in the linkage effect of the cultured cells are too complicated to realize, and the culture The gas environment is not circulated, and the external flow of the culture medium may cause more additional stress on the cultured cells, and the adjustable range of the air pressure is limited

Method used

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  • Double-stress stimulation culture device and method thereof
  • Double-stress stimulation culture device and method thereof

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Embodiment

[0033] A dual stress stimulation culture device for cells, comprising a closed culture chamber (2), in which a section of the elastic culture chamber (1) can be fixed, and the other end is connected to a telescopic rod (3), the telescopic The rod is fixed on the moving part of the linear motion unit (4), so as to realize the reciprocating linear motion of the telescopic rod with controllable speed, cycle and motion mode. During the reciprocating motion of the telescopic rod, the cultivation chamber will not be damaged. airtightness problem; the cultivation chamber is connected with an air pressure regulating device (9), and the air pressure regulating device is composed of a pressure sensor (5) and two air pumps. The outlet of the air pump, the inlet of the air pump used as the exhaust pump (7), the pressure sensor (5) is used to measure the pressure value of the cultivation room, and the control of the telescopic rod and the pump by the computer control system is realized. Th...

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Abstract

The invention belongs to the field of biomedical cell tissue culture and experiments, and discloses a stimulation culture device and a stimulation culture method for applying two stresses including atraction force and a pressure to cells, controllable time sequences are realized for a culture chamber to respectively realize pressurization and pressure extraction, and the same-frequency stretchingand retraction of an elastic culture cavity are matched to realize the joint stimulation of any pressure and tensile strain on the cells in a controllable range, and a simple and effective device anda technical scheme for forming strain and pressure on the cells by the tensile force and carrying out linkage adjustable stimulation culture on the cells at the same time are provided.

Description

technical field [0001] The invention belongs to the field of biomedical cell tissue culture and experiment, discloses a stimulating culture device and method for applying two kinds of stress including tension force and pressure to cells and tissues, and provides a simple and effective way to realize the effect of tension force on cell formation A device and a technical scheme for simultaneous adjustable stimulation and culture of cells by strain and pressure. Background technique [0002] According to the published invention patent application No. 2017107159633, a cell culture device under the associated action of pressure and unidirectional strain and its method, the pressure and unidirectional strain described in the linkage effect of the cultured cells are too complicated to realize, and the culture The gas environment is not circulated, and the use of the external flow of the culture medium may cause more additional stress on the cultured cells, and the adjustable range ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/34
CPCC12M23/38C12M35/04C12M41/40
Inventor 顾宇灵
Owner 上海天引生物科技有限公司
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