61 results about "Biosensor array" patented technology

A biosensor array, system and method for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

Biosensor circuit arrangement including a substrate, a sensor element formed in or on a surface region of the substrate with a physical parameter, which is coupled to a substance to be examined, the type of coupling having a resistive component, the sensor element having an electrically conductive sensor electrode that is coupled to the substance to be examined, the sensor element having a measuring transistor the gate terminal of which is coupled to the electrically conductive sensor electrode, and the physical parameter being the threshold voltage of the measuring transistor, and a calibration device formed in or on the substrate, the calibration device being set up such that it is used to at least partly compensate for an alteration of the value of the physical parameter of the sensor element.

A biosensor array having a substrate, a plurality of biosensor zones arranged on the substrate, each of which has a first terminal and a second terminal, at least one drive line and at least one detection line, the at least one drive line being electrically insulated from the at least one detection line. In each case the first terminal of each biosensor zone is coupled to precisely one of the at least one drive line and the second terminal of each biosensor zone is coupled to precisely one of the at least one detection line, and at least one of the at least one drive line and at least one of the at least one detection line is coupled to at least two of the biosensor zones. The biosensor array also has a drive unit for providing an electrical drive signal, a detection unit for detecting an electrical detection signal resulting from the electrical drive signal, and a selection unit that couples the drive unit to the drive line of a biosensor zone to be selected and the detection unit to the detection line of the biosensor zone to be selected, whereby the biosensor zone is selected.

This invention relates to the detection of bioelectric signals indicative of neuromuscular function. A mapping biosensor array is first used to identify, relative to reliable anatomical landmarks, the optimal myoelectrical detection site for the majority of the population. Then a diagnostic biosensor array is fabricated using this information so that when the diagnostic biosensor array is positioned on an individual using the reliable anatomical landmarks, the detection electrode is automatically positioned over the optimal detection site.

A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

Arrays of biosensors are provided along with methods for operating the arrays of biosensors. The array of biosensors may include a first reference electrode that is connected to an input of a first control amplifier; a first working electrode and a second working electrode in proximity with the first reference electrode; and a counter electrode that is connected to at least an output of the first control amplifier, where the first control amplifier is operative with the counter electrode to maintain a first specified voltage between the first working electrode and the first reference electrode, and between the second working electrode and the first reference electrode. The array of biosensors optionally may further include a second reference electrode that is connected to an input of a second control amplifier, where the second control amplifier is operative with the counter electrode to maintain a second specified voltage between the first working electrode and the second reference electrode, and between the second working electrode and the second reference electrode.

A biosensor array, system and method for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

A biosensor cell (10) and biosensor array comprising a plurality of biosensor cells (10), each biosensor cell (10) comprising a sensing zone. The first sensing electrode (24), a second sensing electrode (25) and the gap (27) separating the sensing electrodes (24,25) are arranged within the sensing zone. The first sensing electrode (24) is electrically insulated from the second sensing electrode (25) by means of the gap (27). Capture molecules (28) are immobilised in the sensing zone; and a field effect transistor (16) having a gate electrode (19), a source electrode (17) and a drain electrode (18); the first sensing electrode (24) being electrically connected to the gate electrode (19) of the field effect transistor (16); and the second sensing electrode (25) being electrically connectable to a gate voltage. The invention also provides a method of detecting a target molecule such as a biomolecule.

A hybrid microfluidic biochip designed to perform multiplexed detection of singled- celled pathogens using a combination of SPR and epi-fluorescence imaging. The device comprises an array of gold spots, each functionalized with a capture biomolecule targeting a specific pathogen. This biosensor array is enclosed by a polydimethylsiloxane (PDMS) microfluidic flow chamber that delivers a magnetically concentrated sample to be tested. The sample is imaged by surface plasmon resonance on the bottom of the biochip, and epi- fluorescence on the top.

A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur. Additionally, the biosensor pixel includes a 1-bit comparator coupled to the trans-impedance amplifier and a 1-bit digital-to-analog converter coupled to the 1-bit comparator, where the 1-bit digital-to-analog converter injects different levels of charge into an input of the trans-impedance amplifier at each cycle based on an output of the 1-bit comparator.

The present invention provides a sensor array device with multiple sensor junctions which have been created through the assembly of two or more differently functionalized surfaces. The functionalizing of the prospective sensor junction areas with sensor compounds occurred when the different surfaces were physically separated from each other before the assembly of the sensor array. By these means, sensor junctions can be built smaller than conventional deposition techniques like printing and photolithography would allow for otherwise. As a consequence, each individual sensor junction contains two potentially different sensor compounds. The sensor array identifies and quantifies different biomolecules.

We provide a sensor array device for the measurement of mixtures of organic compounds comprising an assembly of sensor half elements which have been functionalized through sensor compounds before the assembly. Each individual sensor of the array contains two sensor compounds pounds which are bound at opposite sensor half elements. The molecular recognition is bi-functional. While the amount of sensor compounds increases linearly, the individual sensors increase with the second power.

Arrays of biosensors are provided along with methods for operating the arrays of biosensors. The array of biosensors may include a first reference electrode that is connected to an input of a first control amplifier; a first working electrode and a second working electrode in proximity with the first reference electrode; and a counter electrode that is connected to at least an output of the first control amplifier, where the first control amplifier is operative with the counter electrode to maintain a first specified voltage between the first working electrode and the first reference electrode, and between the second working electrode and the first reference electrode. The array of biosensors optionally may further include a second reference electrode that is connected to an input of a second control amplifier, where the second control amplifier is operative with the counter electrode to maintain a second specified voltage between the first working electrode and the second reference electrode, and between the second working electrode and the second reference electrode.

A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

An optical biosensor pixel for detecting the amount of light that is generated by the biosensing process and a biosensor array architecture that includes such biosensor pixels. The optical biosensor pixel includes a photodiode configured to convert an incident photon flux into a current. Additionally, the optical biosensor pixel includes an optical filter configured to select specific wavelengths and / or photon flux angles to reach the photodiode from a biological sample. The biosensor pixel further includes a trans-impedance amplifier coupled to the photodiode, where the trans-impedance amplifier is configured to convert the current into a voltage signal. Additionally, the biosensor pixel includes a 1-bit comparator coupled to the trans-impedance amplifier and a 1-bit digital-to-analog converter coupled to the 1-bit comparator, where the 1-bit digital-to-analog converter injects different levels of charge into an input of the trans-impedance amplifier at each cycle based on an output of the 1-bit comparator.

The invention discloses an intelligent food safety detection system and detection method. The detection system comprises the following devices: a sampling manipulator, a biosensor array, a sensor system, a signal processing system, an intelligent analytic system and a control system, wherein the sampling manipulator is an operating platform for storing a detected sample; the biosensor array is used for detecting the smell of a food to be detected; the sensor system is used for inspecting the environmental parameters outside the food; the signal processing system is used for converting the detection analog signals of the biosensor array and the sensor system into digital signals through A / D conversion and reshaping the signals; the intelligent analytic system is used for carrying out signal identification processing and quality analysis on the reshaped signals to acquire the quality detection data of the food, and carrying out analysis and comparison with food standards to obtain the result of the food quality; and the control system is used for controlling the above devices to work. The invention can detect in real time and analyze and judge the food quality safely, efficiently and accurately by using digitized analysis and intelligent processing.

A labeled immune biological transducer array system of GMR spin valve comprises solenoid to contain detection module, magnetic field driving circuit connected to solenoid and used to generate constant magnetic field in solenoid, data processing circuit connected to detection module and used to obtain and analyze resistance value variation of GMR spin valve chip, a pair of balance bridge circuit used to detect temperature drift of GMR spin valve chip and to carry out compensation. Its detecting method is also disclosed.

According to one aspect, an integrated magnetic particle measurement device for detecting a presence or absence of magnetic particles in a sample volume includes at least one sensor cell having a differential sensor pair. An active sensor oscillator frequency is responsive to one or more magnetic particles situated within a sample volume. The sensor cell is configured to be operative in the absence of an externally applied magnetic field. A frequency measurement circuit provides as a time-multiplexed output a first count representative of the active sensor oscillator frequency and a second count representative of the reference sensor oscillator frequency. A calculated difference between the first count and the second count is indicative of a presence or an absence of one or more magnetic particles within the sample volume. An integrated magnetic particle measurement system array and a method for detecting one or more magnetic particles are also described.

The magnetic sensor device disclosed herein uses a signal storage magnetic layer for sensing and storing target magnetic field signals. The stored magnetic field signals are then measured by a magnetic sensor.

This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

This present disclosure provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The present disclosure also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

A biosensor, sensor array, sensing method and sensing apparatus are provided in which individual cells or randomly mixed populations of cells, having unique response characteristics to chemical and biological materials, are deployed in a plurality of discrete sites on a substrate. In a preferred embodiment, the discrete sites comprise microwells formed at the distal end of individual fibers within a fiber optic array. The biosensor array utilizes an optically interrogatable encoding scheme for determining the identity and location of each cell type in the array and provides for simultaneous measurements of large number of individual cell respnses to target analyses. The sensing method utilizes the unique ability of cell populations to respond to biologically significant compounds in a characteristic and detectable manner. The biosensor array and measurement method may be employed in the study of biologically active materials in situ environmental monitoring, monitoring of a variety of bioprocesses, and for high throughput screening of large combinatorial chemical libraries.

A photonic biosensor, a photonic biosensor array, and a method of detecting a bio-material using the same are provided. The photonic biosensor includes a light emitting diode configured to emit light, a photodiode (PD), an optical fiber configured to connect the light emitting diode with the PD, and a micro-fluidic channel disposed on the optical fiber. Bio-antibodies or aptamers are fixed to the surface of the optical fiber, and the micro-fluidic channel includes gold (Au) nanoparticles to which bio-antibodies or aptamers are fixed. The photonic biosensor may be configured using absorption of surface plasmons in Au nanoparticles with respect to light traveling through the surface of the optical fiber configured to connect the light emitting diode with the PD, thus simplifying the manufacture of the biosensor and reducing the manufacturing cost.

The invention discloses a current type signal detection analog front end circuit which comprises a band-gap reference source, a current mirror, a buffer, a transimpedance amplifier and a successive approximation analog-to-digital converter, wherein the band-gap reference source is used for generating 10 microamperes current required by the current mirror and providing 300 mV bias voltage, the current mirror loads the mirror image of the 300 mV bias voltage to a magnetic sensitive biosensor array and provides an output path for detection current, the buffer is used for reducing the influence of circuit vibration on the 300 mV bias voltage, the transimpedance amplifier converts the detection current into detection voltage and amplifies the detection voltage, the successive approximation analog-to-digital converter converts the detection voltage into a digital code and outputs the digital code to DSP (Digital Signal Processor), and thus the detection is finished. According to the invention, the mode of single-chip full integration and current detection is adopted, weak variation signals in magnetic sensitive biosensors are extracted effectively, the transimpedance amplifier is used for converting the detection current into the detection voltage and amplifying the detection voltage reasonably, and finally the analog-to-digital converter outputs the digital code.

This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

The invention discloses multichannel biosensor array preparation based on paper chips and immunological detection application. Hydrophobic areas and hydrophilic areas are prepared on paper through utilization of a wax printing technology, and through configuration of insulated layer ink, immune working areas which are absolutely hydrophobic are prepared, so precision and repetition rates of sensors are optimized. Through configuration of corresponding conductive ink, corresponding reference electrodes, working electrodes and counter electrodes are printed on the paper, and the working electrodes are modified by nanogold, so sensitivity and corresponding intervals of the electrodes are improved. The working electrodes are functionalized, specific recognition is carried out on fixed antigensthrough utilization of disease antibodies in sample solution, and specific enzyme-linked immunosorbent assay is carried out through utilization of second antibodies and first antibodies. Through utilization of an electrochemical detection principle, oxidation-reduction reaction is carried out on electrochemical base solution through utilization of enzyme of the second antibodies. Content of to-be-detected antibodies in samples is quantized. High-sensitivity detection of the be-detected antibodies is realized.

Embodiments of the present invention relate to fingerprint scanning. Specifically, the present invention relates to a multi-sided fingerprint scanning device on a card (e.g., credit card, smart card, etc.), an associated energy-efficient method for attaining accurate fingerprint information using a multiple charge-coupled biometric sensor array. In a typical embodiment, a scanning device will be provided that includes a scanning area comprised of a set (e.g., at least one) of imaging pixel electrodes (e.g., arranged adjacent to one another in a grid-like or other fashion). As a user presses his / her finger against the scanning area, a portion of the finger will contact a plurality of electrodes. When this occurs, a voltage source of the device will apply a first voltage to each of the plurality of electrodes. A meter of the device will take a first electrical measurement (e.g., resistance and / or charged skin voltage) of the plurality of electrodes. The voltage source of the device will apply a second voltage to the plurality of electrodes. The meter of the device will take a second electrical measurement (e.g., resistance and / or charged skin voltage) of the plurality of electrodes. The voltage level difference between the first electrical measurement and second electrical measurement is calculated. The voltage level difference provides accurate fingerprint information.

The invention relates to a biosensor array based on an ion sensitive field effective transistor. The biosensor array comprises a row biosensor, a line biosensor, a row decoder circuit, a line decoder circuit and a control logic circuit, wherein the row biosensor is connected with the row decoder circuit, the line biosensor is connected with the line decoder circuit, and the control logic circuit is coupled to the row decoder circuit and the line decoder circuit. The biosensor array based on the ion sensitive field effective transistor, provided by the invention, has the beneficial effects that the detection area is greatly covered, a plurality of targets are simultaneously detected rapidly in real time through measuring a large quantity of sensors inside the biosensor array, the statistical error of the detection is greatly reduced, and thus the accuracy and the repeatability of the detection are improved.