Multichannel biosensor array preparation based on paper chips and immunological detection application

Abstract

The invention discloses multichannel biosensor array preparation based on paper chips and immunological detection application. Hydrophobic areas and hydrophilic areas are prepared on paper through utilization of a wax printing technology, and through configuration of insulated layer ink, immune working areas which are absolutely hydrophobic are prepared, so precision and repetition rates of sensors are optimized. Through configuration of corresponding conductive ink, corresponding reference electrodes, working electrodes and counter electrodes are printed on the paper, and the working electrodes are modified by nanogold, so sensitivity and corresponding intervals of the electrodes are improved. The working electrodes are functionalized, specific recognition is carried out on fixed antigensthrough utilization of disease antibodies in sample solution, and specific enzyme-linked immunosorbent assay is carried out through utilization of second antibodies and first antibodies. Through utilization of an electrochemical detection principle, oxidation-reduction reaction is carried out on electrochemical base solution through utilization of enzyme of the second antibodies. Content of to-be-detected antibodies in samples is quantized. High-sensitivity detection of the be-detected antibodies is realized.

Description

technical field [0001] The invention relates to the field of electrochemical analysis, in particular to the preparation of a multi-channel biosensor array based on a paper chip and the application of immunodetection. Background technique [0002] Disease control and treatment depend on accurate diagnosis, among which, ELISA can be used to detect a variety of disease diagnoses, such as AIDS, tuberculosis, and hepatitis. Enzyme-linked immunosorbent assay is also the most widely used immunological test in clinical diagnosis, it involves the specific interaction between antigen and antibody, and the concentration of analyte is measured by the enzymatic reaction between labeled antibody and substrate. ELISA has high sensitivity and specificity, and can be used to accurately detect a variety of disease markers, such as AIDS, tuberculosis, malaria, and hepatitis B and C. However, the traditional enzyme-linked immunosorbent assay is designed for well-equipped clinical laboratories,...

AI Technical Summary

Problems solved by technology

For example, clinical identification of the origin of an unknown fever that may be attributed to several possible diseases such as tuberculosis, HIV, and mononucleosis often requires the detection of multiple panels of biomarkers, which cannot be met by existing paper-on-a-chip sensor designs for telephone lines. kind of demand

[0005] Moreover, the existing electrochemical sensors often pay more attention to the modification of electrodes to improve detection sensitivity, which cannot meet the high throughput and high repeatability of detection.

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